Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Human coronavirus NL63, Transfected cell line - Human coronavirus NL63, Recombinant fragment - Human coronavirus NL63 samples.
Images
![Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--western-blot-img428913.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)")
![Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--immunocytochemistry-immunofluorescence-img428926.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)")
![Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--indirect-elisa-img428934.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)")
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | I-ELISA | |
|---|---|---|---|
| Human coronavirus NL63 | Predicted | Predicted | Predicted |
| Recombinant fragment - Human coronavirus NL63 | Not recommended | Not recommended | Tested |
| Transfected cell line - Human coronavirus NL63 | Not recommended | Tested | Not recommended |
| Transfected cell lysate - Human coronavirus NL63 | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human coronavirus NL63 | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human coronavirus NL63 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human coronavirus NL63, Recombinant fragment - Human coronavirus NL63 | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human coronavirus NL63 | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human coronavirus NL63 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human coronavirus NL63, Recombinant fragment - Human coronavirus NL63 | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human coronavirus NL63 | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human coronavirus NL63 | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human coronavirus NL63, Transfected cell line - Human coronavirus NL63 | Dilution info - | Notes - |
Target data
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Alternative names
6, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N
Recommended products
Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Human coronavirus NL63, Transfected cell line - Human coronavirus NL63, Recombinant fragment - Human coronavirus NL63 samples.
Key facts
- Isotype
- IgG1
- Host species
- Human
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- abd259
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316213 is the carrirer-free version of Anti-NL63 Nucleoprotein antibody [abd259] ab316212.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NL63 nucleoprotein also known as N protein is a structural protein of the human coronavirus NL63 (HCoV-NL63). This protein plays a role in the viral replication cycle by binding to the viral RNA genome. It helps in packaging RNA into a ribonucleoprotein complex essential for virus assembly and infection. The NL63 nucleoprotein is approximately 60 kDa in size. It is expressed in the cytoplasm of infected cells where it interacts with other viral and host cell proteins.
- Biological function summary
The NL63 nucleoprotein is essential for the stability of the viral RNA genome. It forms a helical ribonucleoprotein complex protecting viral RNA from host cell degradation mechanisms. This protein interacts with the viral polymerase complex to facilitate viral replication and transcription. It is part of a multi-protein complex that includes other viral structural proteins such as membrane and envelope proteins. Through these interactions the nucleoprotein coordinates viral assembly and release from host cells.
- Pathways
The NL63 nucleoprotein interacts with pathways related to viral replication and translation processes in host cells. It is involved in the RNA synthesis pathway where it aids in the synthesis of viral RNA a critical step for viral propagation. The nucleoprotein relates to other viral proteins like the spike glycoprotein which plays a role in virus entry into host cells. Through these pathways the nucleoprotein regulates viral life cycle stages ensuring efficient replication and spread.
- Associated diseases and disorders
The NL63 nucleoprotein is linked to respiratory infections associated with human coronavirus NL63. This virus causes mild to severe respiratory symptoms including respiratory tract infections and croup particularly in children and the elderly. The nucleoprotein has connections to the spike protein in disease progression as the spike protein facilitates virus entry into host cells. Understanding the role of the nucleoprotein in disease mechanisms can assist in the development of antiviral strategies to combat HCoV-NL63 infections.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--western-blot-img428913.jpg "Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)")
Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)
This data was developed using Anti-NL63 Nucleoprotein antibody [abd259] ab316212, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (Anti-NL63 Nucleoprotein antibody [abd259] ab316212) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (Goat Anti-Human IgG H&L (HRP) ab6858) at 1/5000 dilution
Observed band size: 50 kDa, 36 kDa
Exposure time: 1s
![Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--immunocytochemistry-immunofluorescence-img428926.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)")
Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)
This data was developed using Anti-NL63 Nucleoprotein antibody [abd259] ab316212, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T(human embryonic kidney epithelial cell) cells labelling NL63 Nucleoprotein with Anti-NL63 Nucleoprotein antibody [abd259] ab316212 at 1/200 (5.62 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in 293T cells transfected with human coronavirus NL63 Nucleoprotein vector containing a Myc-his-tag® (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 647 Anti-Myc tag antibody [9E10] ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.
![Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213), expandable thumbnail](https://content.abcam.com/products/images/nl63-nucleoprotein-antibody-abd259-bsa-and-azide-free-ab316213--indirect-elisa-img428934.jpg "Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)")
Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (ab316213)
This data was developed using Anti-NL63 Nucleoprotein antibody [abd259] ab316212, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-NL63 Nucleoprotein antibody [abd259] ab316212 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1:1000 dilution.
Antigen: HCoV-NL63 Nucleocapsid protein,MERS-CoV Nucleocapsid protein,SARS-CoV Nucleocapsid protein,HCoV-OC43 Nucleocapsid protein,HCoV-HKU1 Nucleocapsid protein,HCoV-229E Nucleocapsid protein.
Antigen concentration: 2000 ng/ml
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