AB316213

Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free

Recombinant
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Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for WB, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Human coronavirus NL63, Transfected cell line - Human coronavirus NL63, Recombinant fragment - Human coronavirus NL63 samples.

View Alternative Names

6, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

This data was developed using ab316212, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T(human embryonic kidney epithelial cell) cells labelling NL63 Nucleoprotein with ab316212 at 1/200 (5.62 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with human coronavirus NL63 Nucleoprotein vector containing a Myc-his-tag® (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

I-ELISA

Supplier Data

Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

This data was developed using ab316212, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316212 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : HCoV-NL63 Nucleocapsid protein,MERS-CoV Nucleocapsid protein,SARS-CoV Nucleocapsid protein,HCoV-OC43 Nucleocapsid protein,HCoV-HKU1 Nucleocapsid protein,HCoV-229E Nucleocapsid protein.

Antigen concentration : 2000 ng/ml

Supplier Data

Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

This data was developed using ab316212, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (<a href='/en-us/products/primary-antibodies/nl63-nucleoprotein-antibody-abd259-ab316212'>ab316212</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 1s

Unconjugated

Anti-NL63 Nucleoprotein antibody [abd259]

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd259

Isotype

IgG1

Carrier free

Yes

Reacts with

Human coronavirus NL63

Applications

I-ELISA, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human coronavirus NL63": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human coronavirus NL63": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell line - Human coronavirus NL63": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Human coronavirus NL63": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

ab316213 is the carrirer-free version of ab316212.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NL63 nucleoprotein also known as N protein is a structural protein of the human coronavirus NL63 (HCoV-NL63). This protein plays a role in the viral replication cycle by binding to the viral RNA genome. It helps in packaging RNA into a ribonucleoprotein complex essential for virus assembly and infection. The NL63 nucleoprotein is approximately 60 kDa in size. It is expressed in the cytoplasm of infected cells where it interacts with other viral and host cell proteins.

Biological function summary

The NL63 nucleoprotein is essential for the stability of the viral RNA genome. It forms a helical ribonucleoprotein complex protecting viral RNA from host cell degradation mechanisms. This protein interacts with the viral polymerase complex to facilitate viral replication and transcription. It is part of a multi-protein complex that includes other viral structural proteins such as membrane and envelope proteins. Through these interactions the nucleoprotein coordinates viral assembly and release from host cells.

Pathways

The NL63 nucleoprotein interacts with pathways related to viral replication and translation processes in host cells. It is involved in the RNA synthesis pathway where it aids in the synthesis of viral RNA a critical step for viral propagation. The nucleoprotein relates to other viral proteins like the spike glycoprotein which plays a role in virus entry into host cells. Through these pathways the nucleoprotein regulates viral life cycle stages ensuring efficient replication and spread.

The NL63 nucleoprotein is linked to respiratory infections associated with human coronavirus NL63. This virus causes mild to severe respiratory symptoms including respiratory tract infections and croup particularly in children and the elderly. The nucleoprotein has connections to the spike protein in disease progression as the spike protein facilitates virus entry into host cells. Understanding the role of the nucleoprotein in disease mechanisms can assist in the development of antiviral strategies to combat HCoV-NL63 infections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

See full target information N

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

Indirect ELISA - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

Western blot - Anti-NL63 Nucleoprotein antibody [abd259] - BSA and Azide free (AB316213)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise