AB254731

Anti-NLE1/nle antibody

Name: Anti-NLE1/nle antibody (ab254731) | Abcam
Brand: Abcam Limited
SKU: AB254731
Price: 655 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NLE1/nle antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NLE1 aa 250 to C-terminus.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLE1/nle antibody (AB254731)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLE1/nle antibody (AB254731)

Paraffin-embedded human oral mucosa tissue stained for NLE1/nle using ab254731 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NLE1/nle antibody (AB254731)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NLE1/nle antibody (AB254731)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling NLE1/nle using ab254731 at 4μg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-NLE1/nle antibody (AB254731)

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Western blot - Anti-NLE1/nle antibody (ab254731)

Lanes 2 - 3:

Western blot - Anti-NLE1/nle antibody (ab254731) at 0.4 µg/mL

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MW Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 3:

U-251 MG (Human brain glioma cell line) cell lysate

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-NLE1/nle antibody (AB254731)

Lane 1:

Western blot - Anti-NLE1/nle antibody (ab254731)

Lanes 2 - 3:

Western blot - Anti-NLE1/nle antibody (ab254731) at 0.4 µg/mL

Lane 1:

MW Ladder

Lane 2:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 3:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NLE1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NVX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLE1 also known as Notchless homolog 1 is a core protein involved in ribosomal biogenesis and maturation. This protein gets expressed in various tissue types with higher occurrence in actively dividing cells such as in the bone marrow. The exact molecular mass of NLE1 is approximately 78 kDa. NLE1's primary mechanical function involves ensuring the proper assembly and functioning of the ribosomal subunits during protein synthesis.

Biological function summary

The Notchless homolog 1 protein plays a critical role in the regulation of ribosome assembly. NLE1 is a part of the ribosome assembly complex and facilitates the maturation of 60S ribosomal subunit which is important for efficient protein synthesis. By doing so it directly influences cell growth and proliferation. NLE1 can regulate multiple cellular processes by impacting protein synthesis rates within cells.

Pathways

Ribosome biogenesis and Notch signaling involve significant activities by NLE1 in cellular contexts. NLE1 participates in the ribosomal biogenesis pathway important for cell proliferation and growth. Additionally connections with the Notch signaling pathway place NLE1 in interaction with proteins like NOTCH1 which influences cell fate determinations and differentiation events in tissues.

Improper function of NLE1 associates with conditions related to abnormal cell proliferation such as cancer. Elevated levels of NLE1 expression have been observed in several cancer types indicating a potential role in oncogenesis. Furthermore the involvement of NLE1 in certain malignancies has been connected to altered interactions with proteins like NOTCH1 making the signaling and modulation of these pathways a critical area of research in unraveling cancer mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).

See full target information NLE1

Alternative Names

HUSSY-07, NLE1, Notchless protein homolog 1

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Cancer science 114:4535-4547 PubMed37750019

2023

Tetraspanin 1 regulates papillary thyroid tumor growth and metastasis through c-Myc-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihua Han,Changming Xie,Bo Liu,Yan Wang,Rui Pang,Wen Bi,Rinan Sheng,Guoqing He,Lingyu Kong,Jiawei Yu,Zhaoming Ding,Lili Chen,Jinliang Jia,Jiewu Zhang,Chunlei Nie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLE1/nle antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLE1/nle antibody (AB254731)

Immunocytochemistry/ Immunofluorescence - Anti-NLE1/nle antibody (AB254731)

Western blot - Anti-NLE1/nle antibody (AB254731)

Western blot - Anti-NLE1/nle antibody (AB254731)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise