Anti-NLGN4 antibody [EPR26331-43] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-NLGN4 antibody [EPR26331-43] - BSA and Azide free (AB316741)

This data was developed using ab316740, the same antibody clone in a different buffer formulation.

Low expression : HepG2.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32243781).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NLGN4 antibody [EPR26331-43] (<a href='/en-us/products/primary-antibodies/nlgn4-antibody-epr26331-43-ab316740'>ab316740</a>) at 1/1000 dilution

Lane 1:

T47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 80 µg with NFDM/TBST

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 80 µg with NFDM/TBST

Lane 3:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 80 µg with NFDM/TBST

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 80 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/200000 dilution

Observed band size: 100 kDa,80 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NLGN4 antibody [EPR26331-43] - BSA and Azide free (AB316741)

This data was developed using ab316740, the same antibody clone in a different buffer formulation.

Low expression : liver.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32243781).

The identity of the lower MW band at approximately 20 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NLGN4 antibody [EPR26331-43] (<a href='/en-us/products/primary-antibodies/nlgn4-antibody-epr26331-43-ab316740'>ab316740</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 40 µg with NFDM/TBST

Lane 2:

Human hypothalamus tissue lysate at 40 µg with NFDM/TBST

Lane 3:

Human hippocampus tissue lysate at 40 µg with NFDM/TBST

Lane 4:

Human cerebellum tissue lysate at 40 µg with NFDM/TBST

Lane 5:

Human liver tissue lysate at 40 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,100 kDa,36 kDa

true

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-NLGN4 antibody [EPR26331-43] - BSA and Azide free (AB316741)

This data was developed using ab316740, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NLGN4 antibody [EPR26331-43] (<a href='/en-us/products/primary-antibodies/nlgn4-antibody-epr26331-43-ab316740'>ab316740</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a His-tag, whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

293T cells transfected with a human NLGN4X expression vector containi a His-tag, whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

293T cells transfected with a human NLGN4Y expression vector containi a His-tag, whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-NLGN4 antibody [EPR26331-43] - BSA and Azide free (AB316741)

This data was developed using ab316740, the same antibody clone in a different buffer formulation.

This antibody does not cross-react with human Neuroligin 1, Neuroligin 2 and Neuroligin 3.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NLGN4 antibody [EPR26331-43] (<a href='/en-us/products/primary-antibodies/nlgn4-antibody-epr26331-43-ab316740'>ab316740</a>) at 1/1000 dilution

Lane 1:

His-tagged human Neuroligin 4 fragment at 10 ng with NFDM/TBST

Lane 2:

His-tagged human Neuroligin 1v2 recombinant protein at 10 ng with NFDM/TBST

Lane 3:

His-tagged human Neuroligin 2 recombinant protein at 10 ng with NFDM/TBST

Lane 4:

His-tagged human Neuroligin 3 recombinant protein at 10 ng with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 15s