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AB97642

Anti-NLK antibody

4

(4 Reviews)

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(11 Publications)

Rabbit Polyclonal NLK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NLK aa 250-500.

View Alternative Names

LAK1, NLK, Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (AB97642)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (AB97642)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97642 at 1/100 dilution. Lower image is co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (AB97642)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (AB97642)

Paraffin embedded mouse ovary tissue stained for NLK using ab97642 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-NLK antibody (AB97642)
  • WB

Unknown

Western blot - Anti-NLK antibody (AB97642)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-NLK antibody (ab97642) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 58 kDa

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Western blot - Anti-NLK antibody (AB97642)
  • WB

Supplier Data

Western blot - Anti-NLK antibody (AB97642)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NLK antibody (ab97642) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 58 kDa

false

Western blot - Anti-NLK antibody (AB97642)
  • WB

Supplier Data

Western blot - Anti-NLK antibody (AB97642)

10% SDS-PAGE

All lanes:

Western blot - Anti-NLK antibody (ab97642) at 1/500 dilution

Lane 1:

Non-transfected HEK-293T whole cell extract at 30 µg

Lane 2:

Transfected HEK-293T whole cell extract at 30 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NLK aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

Q9UBE8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLK (Nemo-Like Kinase) functions as a serine/threonine protein kinase involved in various signaling pathways. It alternatively is known as nemo-like protein or nlk. The molecular mass of NLK is approximately 54 kDa. Expression of NLK occurs in various tissues but it is highly observed in the brain and testis. This wide expression suggests that NLK may have diverse functional roles.
Biological function summary

NLK participates in regulating cellular processes such as cell cycle apoptosis and transcription. It is not part of a larger complex but works within signaling pathways to influence these processes. NLK modulates the activity of several transcription factors particularly in the Wnt/beta-catenin signaling pathway where it negatively regulates the signaling to control gene expression relevant to cell proliferation and differentiation.

Pathways

NLK is an important player in the Wnt/beta-catenin and MAPK pathways. In the Wnt/beta-catenin pathway it associates with proteins such as TCF/LEF and beta-catenin acting to modulate transcription by phosphorylating these molecules. The MAPK pathway involves NLK in response to stress signals coordinating with proteins like TAK1 to mediate cellular responses. These pathways illustrate NLK’s role in transmitting signals that govern cell fate and stress responses.

NLK has links to cancer and neurodegenerative diseases. Alterations in NLK expression or function can contribute to the development and progression of cancers by affecting the regulation of cell proliferation and apoptosis. Specifically dysregulation in the Wnt/beta-catenin pathway in which NLK acts relates to colorectal cancers. In the context of neurodegenerative diseases NLK has connections with the protein TDP-43 which is implicated in diseases such as amyotrophic lateral sclerosis highlighting its role in neuronal health and potential links to neuronal degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination (PubMed : 12482967, PubMed : 14960582, PubMed : 15004007, PubMed : 15764709, PubMed : 20061393, PubMed : 20874444, PubMed : 21454679). Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2 (PubMed : 15004007, PubMed : 15764709). Negative regulator of the canonical Wnt/beta-catenin signaling pathway (PubMed : 12482967). Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 (PubMed : 21454679). Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes (PubMed : 12482967, PubMed : 21454679). Negative regulator of the Notch signaling pathway (PubMed : 20118921). Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1 (PubMed : 20118921). Negative regulator of the MYB family of transcription factors (PubMed : 15082531). Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP (PubMed : 15082531). Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself (PubMed : 15082531). Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 (PubMed : 15004007, PubMed : 15764709). Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members (PubMed : 25512613). Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed : 25512613). Acts as an inhibitor of the mTORC1 complex in response to osmotic stress by mediating phosphorylation of RPTOR, thereby preventing recruitment of the mTORC1 complex to lysosomes (PubMed : 26588989).
See full target information NLK
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:25 PubMed39837814

2025

Targeting SRSF1 improves cancer immunotherapy by dually acting on CD8T and tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gui-Qi Zhu,Zheng Tang,Tian-Hao Chu,Biao Wang,Shi-Ping Chen,Chen-Yang Tao,Jia-Liang Cai,Rui Yang,Wei-Feng Qu,Yi Wang,Qian-Fu Zhao,Run Huang,Meng-Xin Tian,Yuan Fang,Jun Gao,Xiao-Ling Wu,Jian Zhou,Wei-Ren Liu,Zhi Dai,Ying-Hong Shi,Jia Fan

The Journal of biological chemistry 300:107542 PubMed38992436

2024

Activation of nemo-like kinase in diamond blackfan anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Wilkes,Aya Shibuya,Y Lucy Liu,Kailen Mark,Jaqueline Mercado,Mallika Saxena,Ryan S Sathianathen,Hye Na Kim,Bertil Glader,Paraic Kenny,Kathleen M Sakamoto

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22926 PubMed37052733

2023

Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Liu,Yiling Cao,Chun Zhang,Hua Su

The Journal of biological chemistry 297:100988 PubMed34298020

2021

The active component of ginseng, ginsenoside Rb1, improves erythropoiesis in models of Diamond-Blackfan anemia by targeting Nemo-like kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Wilkes,Kevin Jung,Britney E Lee,Mallika Saxena,Ryan S Sathianathen,Jacqueline D Mercado,Cristina Perez,Johan Flygare,Anupama Narla,Bertil Glader,Kathleen M Sakamoto

Experimental hematology 91:65-77 PubMed32926965

2020

Metformin-induced suppression of Nemo-like kinase improves erythropoiesis in preclinical models of Diamond-Blackfan anemia through induction of miR-26a.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Wilkes,Kavitha Siva,Gianluca Varetti,Jacqueline Mercado,Ethan P Wentworth,Cristina A Perez,Mallika Saxena,Sharon Kam,Simryn Kapur,Jun Chen,Anu Narla,Bert Glader,Shou Lin,Manuel Serrano,Johan Flygare,Kathleen M Sakamoto

Nature communications 11:3344 PubMed32620751

2020

Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase.

Applications

Unspecified application

Species

Unspecified reactive species

M C Wilkes,K Siva,J Chen,G Varetti,M Y Youn,H Chae,F Ek,R Olsson,T Lundbäck,D P Dever,T Nishimura,A Narla,B Glader,H Nakauchi,M H Porteus,C E Repellin,H T Gazda,S Lin,M Serrano,J Flygare,K M Sakamoto

OncoTargets and therapy 11:2875-2890 PubMed29844685

2018

RIP1 regulates TNF-α-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-κB-VEGF-C pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Zong Li,Xiao-Jie Jiang,Bin Lin,Hai-Jie Hong,Si-Yuan Zhu,Lei Jiang,Xiao-Qian Wang,Nan-Hong Tang,Fei-Fei She,Yan-Ling Chen

Nature communications 7:12885 PubMed27698350

2016

A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Tianzhi Huang,Angel A Alvarez,Rajendra P Pangeni,Craig M Horbinski,Songjian Lu,Sung-Hak Kim,C David James,Jeffery J Raizer,John A Kessler,Cameron W Brenann,Erik P Sulman,Gaetano Finocchiaro,Ming Tan,Ryo Nishikawa,Xinghua Lu,Ichiro Nakano,Bo Hu,Shi-Yuan Cheng

Molecular carcinogenesis 56:945-959 PubMed27583705

2016

CACNA2D3 is downregulated in gliomas and functions as a tumor suppressor.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Jin,Daming Cui,Jie Ren,Ke Wang,Tao Zeng,Liang Gao

Cellular physiology and biochemistry : internation 39:1187-95 PubMed27577116

2016

Impact of miR-208 and its Target Gene Nemo-Like Kinase on the Protective Effect of Ginsenoside Rb1 in Hypoxia/Ischemia Injuried Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Yan,Jianxun Liu,Hongjin Wu,Yuna Liu,Sidao Zheng,Chengying Zhang,Cui Yang
View all publications
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