AB119802

Anti-NLN antibody [OTI1D6]

Name: Anti-NLN antibody [OTI1D6] (ab119802) | Abcam
Brand: Abcam Limited
SKU: AB119802

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(3 Publications)

Mouse Monoclonal NLN antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Transfected cell lysate - Human, Human, African green monkey, Transfected cell line - Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NLN.

View Alternative Names

AGTBP, KIAA1226, NLN, Angiotensin-binding protein, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurotensin endopeptidase, MEP

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human tonsil tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human pancreas carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human bladder carcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for NLN using ab119802 at 1/150 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells using ab119802 at 1/100 dilution stained for NLN (Red) compared to a nonspecific negetive control antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY NLN, stained for NLN (green) using ab119802 at 1/100 dilution in ICC/IF.

Unknown

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

All lanes:

Western blot - Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 7:

MDCK cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 81 kDa

false

Unknown

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

All lanes:

Western blot - Anti-NLN antibody [OTI1D6] (ab119802) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY NLN cDNA at 5 µg

Predicted band size: 81 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1D6

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human, African green monkey

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NLN.

Q9BYT8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The clone number has been updated from 1D6 to OTI1D6, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLN also known as Neurolysin is a 77 kDa metalloendopeptidase enzyme with an ability to cleave neuropeptides at specific sites. Researchers frequently observe NLN in the central nervous system kidneys testis and muscle tissues. This enzyme notably exists as a mitochondrial matrix protein showing an evolutionary conservation in its structure across mammalian species. NLN plays a role in processing bioactive peptides displaying similarity in substrate specificity to its better-known counterpart thimet oligopeptidase.

Biological function summary

NLN degrades neuropeptides which aids in regulating their activity and maintaining homeostasis. It does not typically form part of a large complex but interacts directly with peptide substrates to exert its effects. By modulating peptide availability NLN influences neurological functions and potentially affects signaling pathways involved in mood pain and energy regulation. Researchers continue to explore NLN's physiological roles and interactions though its exact contributions outside peptide processing remain less detailed.

Pathways

Studies show NLN engages in the metabolism of bioactive peptides including enkephalins and tachykinins. In these pathways NLN acts to terminate peptide signaling by cleaving them which alters receptor interactions. Within these mechanisms NLN shares activity with proteins like neprilysin both contributing to peptide degradation but acting on different substrates or within distinct cellular locales. These pathways are critical for maintaining neural function and homeostasis underlying many processes in the nervous system.

Dysregulation of NLN activity links to conditions such as hypertension and Alzheimer's disease. In hypertension improper peptide degradation can affect blood pressure regulation. In Alzheimer’s disease NLN’s inability to regulate amyloid-beta peptides in association with neprilysin contributes to plaque accumulation a hallmark of the disease. Understanding NLN’s role offers potential therapeutic insights for correcting peptide imbalances related to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A (By similarity). Acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity).

See full target information NLN

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 10:120 PubMed38906862

2024

Enhancing mitochondrial proteolysis alleviates alpha-synuclein-mediated cellular toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhang,Linhao Ruan,Hu Wang,Jin Zhu,Taibo Li,Gordon Sun,Yi Dong,Yuhao Wang,Gil Berreby,Ashley Shay,Rong Chen,Sreekumar Ramachandran,Valina L Dawson,Ted M Dawson,Rong Li

Science translational medicine 12: PubMed32269163

2020

The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Mirali,Aaron Botham,Veronique Voisin,Changjiang Xu,Jonathan St-Germain,David Sharon,Fieke W Hoff,Yihua Qiu,Rose Hurren,Marcela Gronda,Yulia Jitkova,Boaz Nachmias,Neil MacLean,Xiaoming Wang,Andrea Arruda,Mark D Minden,Terzah M Horton,Steven M Kornblau,Steven M Chan,Gary D Bader,Brian Raught,Aaron D Schimmer

Scientific reports 6:28176 PubMed27307030

2016

Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer.

Applications

Species

Unspecified reactive species

Kuo-Wang Tsai,Chung-Man Leung,Yi-Hao Lo,Ting-Wen Chen,Wen-Ching Chan,Shou-Yu Yu,Ya-Ting Tu,Hing-Chung Lam,Sung-Chou Li,Luo-Ping Ger,Wen-Shan Liu,Hong-Tai Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLN antibody [OTI1D6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLN antibody [OTI1D6] (AB119802)

Flow Cytometry (Intracellular) - Anti-NLN antibody [OTI1D6] (AB119802)

Immunocytochemistry/ Immunofluorescence - Anti-NLN antibody [OTI1D6] (AB119802)

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

Western blot - Anti-NLN antibody [OTI1D6] (AB119802)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise