Anti-NLP1 antibody [EPR16545] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NLP1 with ab192609 at 1/100 dilution (6.3μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue). Negative control also shown.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling NLP1 with ab192609 at 1/100 dilution (6 μg/ml); followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NLP1 antibody [EPR16545] (<a href='/en-us/products/primary-antibodies/nlp1-antibody-epr16545-ab192609'>ab192609</a>) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab192609 at 1/40 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NLP1 antibody [EPR16545] (<a href='/en-us/products/primary-antibodies/nlp1-antibody-epr16545-ab192609'>ab192609</a>)

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NLP1 antibody [EPR16545] (<a href='/en-us/products/primary-antibodies/nlp1-antibody-epr16545-ab192609'>ab192609</a>) at 1/10000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-NLP1 antibody [EPR16545] - BSA and Azide free (AB251143)

This data was developed using ab192609, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NLP1 antibody [EPR16545] (<a href='/en-us/products/primary-antibodies/nlp1-antibody-epr16545-ab192609'>ab192609</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Lane 5:

Mouse brain tissue lysate at 10 µg

Lane 6:

Mouse heart tissue lysate at 10 µg

Lane 7:

Mouse kidney tissue lysate at 10 µg

Lane 8:

Mouse spleen tissue lysate at 10 µg

Lane 9:

Rat brain tissue lysate at 10 µg

Lane 10:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false