Rabbit Polyclonal NLRC4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NLRC4 conjugated to Keyhole Limpet Haemocyanin.
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- Frontiers in immunology 12:6748082021A Novel Mutation in the NBD Domain of Causes Mild Autoinflammation With Recurrent Urticaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Wang,Wen Wen,Mengyue Deng,Yue Li,Gan Sun,Xiaodong Zhao,Xuemei Tang,Huawei MaoPubMed 34248956
- Pediatric surgery international 35:15-202018Altered expression of inflammasomes in Hirschsprung's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroki Nakamura,Anne Marie O'Donnell,Naoum Fares Marayati,Christian Tomuschat,David Coyle,Prem PuriPubMed 30386901
- Nature medicine 23:174-1842017Expression of specific inflammasome gene modules stratifies older individuals into two extreme clinical and immunological states.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Furman et. al.PubMed 28092664
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NLRC4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPP4
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Target data
Function
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
Alternative names
CARD12, CLAN, CLAN1, IPAF, UNQ6189/PRO20215, NLRC4, NLR family CARD domain-containing protein 4, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, CED-4-like protein Clan, Ipaf
Recommended products
Rabbit Polyclonal NLRC4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NLRC4 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NLRC4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPP4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NLRC4 also known as CARD12 or IPAF is an intracellular protein that plays an important role in inflammation and immune responses. It belongs to the NOD-like receptor (NLR) family specifically the NLRC subfamily and contains a caspase activation and recruitment domain (CARD). NLRC4 weighs approximately 120 kDa and is expressed abundantly in macrophages and dendritic cells. It participates mechanically by recognizing cytosolic pathogen-associated molecular patterns (PAMPs) and activating the immune response.
- Biological function summary
NLRC4 forms part of the inflammasome complex which is a multi-protein unit essential for innate immunity. This protein helps detect and respond to bacterial components enabling the activation of caspase-1. Caspase-1 then processes pro-inflammatory cytokines like interleukin-1β and interleukin-18 into their active forms. This mechanism helps defend against bacterial infections and maintain tissue homeostasis.
- Pathways
NLRC4 functions within the inflammasome activation pathway and intersects with autophagy processes. It facilitates the detection of intracellular flagellin through its interaction with the NAIP proteins leading to the activation of caspase-1. NLRC4's activity results in inflammasome assembly which links it to both the innate immune response and cell death pathways. This association positions it near proteins such as ASC which acts as an adapter molecule in inflammasomes.
- Associated diseases and disorders
NLRC4 is linked to autoinflammatory conditions and gastrointestinal diseases. Mutations or dysregulation in NLRC4 may result in the development of autoinflammatory syndromes characterized by recurrent fever and systemic inflammation. These conditions associate with other proteins in the inflammasome complex like NLRP3 which also mediates inflammatory responses. Furthermore disturbances in NLRC4 function can contribute to digestive system diseases emphasizing its relevance in maintaining balanced immune activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC4 antibody (ab115537)
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115537 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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