AB201792

Anti-NLRC4 antibody [EPR19733]

RabMAb
Recombinant
KO Validated
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(26 Publications)

Rabbit Recombinant Monoclonal NLRC4 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 26 publications.

View Alternative Names

CARD12, CLAN, CLAN1, IPAF, UNQ6189/PRO20215, NLRC4, NLR family CARD domain-containing protein 4, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, CED-4-like protein Clan, Ipaf

4 Images

Lab

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/200 dilution

All lanes:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Blocking/Dilution buffer : 5% NFDM/TBST.

NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID : 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University

All lanes:

Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

Lane 1:

RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC at 10 µg

Lane 2:

RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

All lanes:

Mouse spleen tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 0.5 second.

The cell lysates were kindly provided by our collaborator Dr. Jiahuai Han, Xiamen University

All lanes:

Western blot - Anti-NLRC4 antibody [EPR19733] (ab201792) at 1/1000 dilution

Lane 1:

293T whole cell lysate (Human epithelial cell line from embryonic kidney) at 10 µg

Lane 2:

293T whole cell lysate transfected with mouse NLRC4 at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

false

Carrier free

Anti-NLRC4 antibody [EPR19733] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19733

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NLRC4 also known as CARD12 or IPAF is an intracellular protein that plays an important role in inflammation and immune responses. It belongs to the NOD-like receptor (NLR) family specifically the NLRC subfamily and contains a caspase activation and recruitment domain (CARD). NLRC4 weighs approximately 120 kDa and is expressed abundantly in macrophages and dendritic cells. It participates mechanically by recognizing cytosolic pathogen-associated molecular patterns (PAMPs) and activating the immune response.

Biological function summary

NLRC4 forms part of the inflammasome complex which is a multi-protein unit essential for innate immunity. This protein helps detect and respond to bacterial components enabling the activation of caspase-1. Caspase-1 then processes pro-inflammatory cytokines like interleukin-1β and interleukin-18 into their active forms. This mechanism helps defend against bacterial infections and maintain tissue homeostasis.

Pathways

NLRC4 functions within the inflammasome activation pathway and intersects with autophagy processes. It facilitates the detection of intracellular flagellin through its interaction with the NAIP proteins leading to the activation of caspase-1. NLRC4's activity results in inflammasome assembly which links it to both the innate immune response and cell death pathways. This association positions it near proteins such as ASC which acts as an adapter molecule in inflammasomes.

NLRC4 is linked to autoinflammatory conditions and gastrointestinal diseases. Mutations or dysregulation in NLRC4 may result in the development of autoinflammatory syndromes characterized by recurrent fever and systemic inflammation. These conditions associate with other proteins in the inflammasome complex like NLRP3 which also mediates inflammatory responses. Furthermore disturbances in NLRC4 function can contribute to digestive system diseases emphasizing its relevance in maintaining balanced immune activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed : 15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

See full target information NLRC4

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:3585885 PubMed40726812

2025

G Protein-Coupled Receptor 30 Attenuates Neuronal Pyroptosis Induced by Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Peng,Xiqi Hu,Jun He,Ying Xia

Scientific reports 15:19037 PubMed40447675

2025

Shikonin inhibits NLRP3 inflammasome activation and controls inflammatory disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Mengmeng Dai,Xinyu Huang,Wenqing Qi,Jing Jing,Jiayi He,Zhizhuo Lu,Chaofang Zhang,Guiling Wang,Run Cao,Yuanfang Ma,Hailong Zhang

Cell reports 44:115479 PubMed40158217

2025

Pyroptosis of pulmonary fibroblasts and macrophages through NLRC4 inflammasome leads to acute respiratory failure.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Guoying Zhang,Brittany Dong,Ankit Pandeya,Jian Cui,Samuel Dos Santos Valenca,Ling Yang,Jiaqian Qi,Zhuodong Chai,Congqing Wu,Daniel Kirchhofer,Toshihiko Shiroishi,Fadi Khasawneh,Min Tao,Feng Shao,Christopher M Waters,Yinan Wei,Zhenyu Li

Journal of translational medicine 23:237 PubMed40016828

2025

FlaA N/C attenuates radiation-induced lung injury by promoting NAIP/NLRC4/ASC inflammasome autophagy and inhibiting pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shihua Deng,Yueyan Yang,Shuang He,Zixin Chen,Xun Xia,Ting Zhang,Qing Yin,Teng Liu,Dongming Wu,Kejian Pan,Ying Xu

Inflammation 48:902-918 PubMed39052181

2024

EZH2-H3K27me3-Mediated Epigenetic Silencing of DKK1 Induces Nucleus Pulposus Cell Pyroptosis in Intervertebral Disc Degeneration by Activating NLRP3 and NAIP/NLRC4.

Applications

Unspecified application

Species

Unspecified reactive species

Qijun Yao,Yue Lei,Yongxu Zhang,Haoran Chen,Xiaowei Dong,Zhiqiang Ye,Haidong Liang

The journal of gene medicine 26:e3683 PubMed38571451

2024

NLRC4-mediated pyroptosis was involved in coagulation disorders of acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Sunkuan Hu,Tiesu Lin,Yufeng Chen,Yimo Guo,Xuecheng Sun,Lingyan Shi,Jingye Pan

Infection and immunity 92:e0050523 PubMed38477589

2024

Enteritidis antitoxin DinJ inhibits NLRP3-dependent canonical inflammasome activation in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Gu,Ang Li,Xirui Zang,Tingting Huang,Yaxin Guo,Xinan Jiao,Zhiming Pan

Journal of neuroinflammation 20:304 PubMed38110963

2023

Targeting CB2R in astrocytes for Parkinson's disease therapy: unraveling the Foxg1-mediated neuroprotective mechanism through autophagy-mediated NLRP3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhu,Feng Xiao,Yao Xiao,Yun Guo,Xuesong Shan,Zhe Zhang,Lieliang Zhang,Hua Guo

mBio 14:e0206623 PubMed37787552

2023

Phosphoprotein phosphatase activity positively regulates oligomeric pyrin to trigger inflammasome assembly in phagocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Haleema S Malik,Flora Magnotti,Nicole A Loeven,Jose M Delgado,Arminja N Kettenbach,Thomas Henry,James B Bliska

Journal of advanced research 52:219-232 PubMed37586642

2023

Targeting Desulfovibrio vulgaris flagellin-induced NAIP/NLRC4 inflammasome activation in macrophages attenuates ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping An,Zihan Zhai,Xin Wang,Yiyun Ding,Linlin He,Lingfeng Li,Qi Mo,Chenlu Mu,Runxiang Xie,Tianyu Liu,Weilong Zhong,Bangmao Wang,Hailong Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLRC4 antibody [EPR19733]

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Western blot - Anti-NLRC4 antibody [EPR19733] (AB201792)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

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