AB223143

Anti-NLRC4 antibody [EPR19733] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal NLRC4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Transfected cell lysate - Mouse samples. Cited in 1 publication.

View Alternative Names

CARD12, CLAN, CLAN1, IPAF, UNQ6189/PRO20215, NLRC4, NLR family CARD domain-containing protein 4, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, CED-4-like protein Clan, Ipaf

1 Images

Supplier Data

Western blot - Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (AB223143)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201792).

Blocking/Dilution buffer : 5% NFDM/TBST.

NLRC4 regulation involves mechanisms that require ASC (apoptosis-associated speck-like protein containing a CARD), a common adaptor protein for several inflammasomes - PMID : 25775556.
The cell lysates were kindly provided by our Collaborator Dr. Jiahuai Han, Xiamen University

All lanes:

Western blot - Anti-NLRC4 antibody [EPR19733] (<a href='/en-us/products/primary-antibodies/nlrc4-antibody-epr19733-ab201792'>ab201792</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 whole cell lysate (Mouse macrophage cell line transformed with Abelson murine leukemia virus) overexpressed with ASC at 10 µg

Lane 2:

RAW 264.7 whole cell lysate NLRC4 KO overexpressed with ASC at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

false

Exposure time: 3min

Unconjugated

Anti-NLRC4 antibody [EPR19733]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19733

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab223143 is the carrier-free version of ab201792.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NLRC4 also known as CARD12 or IPAF is an intracellular protein that plays an important role in inflammation and immune responses. It belongs to the NOD-like receptor (NLR) family specifically the NLRC subfamily and contains a caspase activation and recruitment domain (CARD). NLRC4 weighs approximately 120 kDa and is expressed abundantly in macrophages and dendritic cells. It participates mechanically by recognizing cytosolic pathogen-associated molecular patterns (PAMPs) and activating the immune response.

Biological function summary

NLRC4 forms part of the inflammasome complex which is a multi-protein unit essential for innate immunity. This protein helps detect and respond to bacterial components enabling the activation of caspase-1. Caspase-1 then processes pro-inflammatory cytokines like interleukin-1β and interleukin-18 into their active forms. This mechanism helps defend against bacterial infections and maintain tissue homeostasis.

Pathways

NLRC4 functions within the inflammasome activation pathway and intersects with autophagy processes. It facilitates the detection of intracellular flagellin through its interaction with the NAIP proteins leading to the activation of caspase-1. NLRC4's activity results in inflammasome assembly which links it to both the innate immune response and cell death pathways. This association positions it near proteins such as ASC which acts as an adapter molecule in inflammasomes.

NLRC4 is linked to autoinflammatory conditions and gastrointestinal diseases. Mutations or dysregulation in NLRC4 may result in the development of autoinflammatory syndromes characterized by recurrent fever and systemic inflammation. These conditions associate with other proteins in the inflammasome complex like NLRP3 which also mediates inflammatory responses. Furthermore disturbances in NLRC4 function can contribute to digestive system diseases emphasizing its relevance in maintaining balanced immune activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed : 15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

See full target information NLRC4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:789-798 PubMed29845235

2018

Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR‑γ and UCP2.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Rong Lin,Langtao Guo,Meiman Hong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLRC4 antibody [EPR19733] - BSA and Azide free

Western blot - Anti-NLRC4 antibody [EPR19733] - BSA and Azide free (AB223143)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise