AB105411

Anti-NLRC5 antibody

Name: Anti-NLRC5 antibody (ab105411) | Abcam
Brand: Abcam Limited
SKU: AB105411
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(22 Publications)

Rabbit Polyclonal NLRC5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human NLRC5.

View Alternative Names

NOD27, NOD4, NLRC5, Protein NLRC5, Caterpiller protein 16.1, Nucleotide-binding oligomerization domain protein 27, Nucleotide-binding oligomerization domain protein 4, CLR16.1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NLRC5 With ab105411.

Positive cytoplasmic staining on human kidney

The section was incubated with ab105411 at 1 μg/ml overnight at 4°C.

A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Heat mediated antigen retrieval was performed with Sodium-Citrate buffer (pH 6.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NLRC5 With ab105411.

Positive cytoplasmic staining on mouse brain.

The section was incubated with ab105411 at 1 μg/ml overnight at 4°C.

A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Heat mediated antigen retrieval was performed with Sodium-Citrate buffer (pH 6.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NLRC5 With ab105411.

Positive cytoplasmic staining on rat kidney.

The section was incubated with ab105411 at 1 μg/ml overnight at 4°C.

A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Heat mediated antigen retrieval was performed with Sodium-Citrate buffer (pH 6.0).

Supplier Data

Western blot - Anti-NLRC5 antibody (AB105411)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NLRC5 antibody (ab105411) at 1 µg/mL

All lanes:

EL4 Cells (mouse T-cell lymphoma cell line at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Observed band size: 200 kDa,150 kDa,80 kDa

false

Unknown

Western blot - Anti-NLRC5 antibody (AB105411)

All lanes:

Western blot - Anti-NLRC5 antibody (ab105411) at 1 µg/mL

All lanes:

EL4 cell lysate

Predicted band size: 204 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NLRC5. The exact immunogen used to generate this antibody is proprietary information.

Q86WI3

Specificity

ab105411 is predicted to not cross react with other NLRP family members.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLRC5 also known as NOD-like receptor family CARD domain-containing 5 is an important protein in the immune response. It has a molecular mass of approximately 186 kDa. This protein locates primarily in the cytoplasm of cells with high expression levels in immune tissues such as the spleen and lymph nodes. Mechanically NLRC5 acts as a transcriptional regulator controlling the expression of major histocompatibility complex (MHC) class I genes. This function plays a critical role in the immune system by facilitating the presentation of endogenous antigens.

Biological function summary

NLRC5 engages in enhancing immune surveillance and antiviral responses by regulating MHC class I transcription. It acts as part of the larger NLR family of proteins which are known to participate in the immune system's response to pathogens. In addition to its role in the immune response NLRC5 can interact with components of the inflammasome complex although its presence in the inflammasome remains controversial. These mechanisms are essential in initializing and sustaining an immune response against infections.

Pathways

NLRC5 involves itself in the interferon signaling pathway and antigen processing and presentation pathways. In the context of interferon signaling NLRC5 influences the activation of genes that drive antiviral states by interacting with proteins such as IRF1 and STAT1. This regulatory role positions NLRC5 as a bridge between innate and adaptive immune responses ensuring proper signaling and presentation of antigens to T cells for effective immune resolution.

NLRC5 exhibits alterations in conditions such as some auto-inflammatory disorders and cancer. Deregulation of NLRC5 can contribute to diseases like systemic lupus erythematosus where its altered expression affects immune function and tolerance. In certain cancers such as hepatocellular carcinoma NLRC5 interacts with oncogenic pathways influencing tumor growth and immune evasion. Researchers explore connections between NLRC5 and proteins like MHC class I molecules to understand their roles in disease mechanisms and therapeutic potentials.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms.

See full target information NLRC5

style

left-column

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115561 PubMed40250424

2025

Epigenomic regulation of stemness contributes to the low immunogenicity of the most mutated human cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Oka,Sabrina S Smith,Valeria S Oliver-Garcia,Truelian Lee,Heehwa G Son,Mahsa Mortaja,Marjan Azin,Anna C Garza-Mayers,Jennifer T Huang,Rosalynn M Nazarian,Thomas D Horn,Shadmehr Demehri

Communications biology 8:292 PubMed39988583

2025

Targeting NLRC5 in cardiomyocytes protects postinfarction cardiac injury by enhancing autophagy flux through the CAVIN1/CAV1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng Gu,Sibo Wang,Liuhua Zhou,Wenjing Wang,Yulin Bao,Ye He,Tongtong Yang,Jiateng Sun,Qiqi Jiang,Tiankai Shan,Chong Du,Zemu Wang,Hao Wang,Liping Xie,Aihua Gu,Yang Zhao,Yong Ji,Qiming Wang,Liansheng Wang

Immunity, inflammation and disease 12:e1361 PubMed39092772

2024

17β-estradiol promotes the progression of temporomandibular joint osteoarthritis by regulating the FTO/IGF2BP1/m6A-NLRC5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xintong Xue,Changyi Li,Shuang Chen,Yan Zheng,Fan Zhang,Yan Xu

Scientific reports 14:15353 PubMed38961101

2024

NLRC5 promotes tumorigenesis by regulating the PI3K/AKT signaling pathway in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ling,Jiahua Chen,Lei Zhan,Juanjuan Fu,Runhua He,Wenyan Wang,Bing Wei,Xiaofeng Ma,Yunxia Cao

International journal of molecular sciences 24: PubMed37686068

2023

NLRC5 Deficiency Reduces LPS-Induced Microglial Activation via Inhibition of NF-κB Signaling and Ameliorates Mice's Depressive-like Behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Sun,Yuqing Shen,Piaopiao Liu,Yi Shen,Yue Hu,Ping Li,Ying Zhang,Fengqin Miao,Jianqiong Zhang

Autophagy 19:1332-1347 PubMed36126167

2022

NLRC5 restricts dengue virus infection by promoting the autophagic degradation of viral NS3 through E3 ligase CUL2 (cullin 2).

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Hao,Jinqian Li,Zhenzhen Zhang,Yang Yang,Qing Zhou,Tiantian Wu,Tongling Chen,Zhongdao Wu,Ping Zhang,Jun Cui,Yi-Ping Li

Technology in cancer research & treatment 21:15330338221112742 PubMed35880269

2022

NLRC5 Might Promote Endometrial Cancer Progression by Inducing PD-L1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Ding Zhu,Jing Zhang,Xiao-Jing Liu,Jun-Hui Zhang,Bing Wei,Wen-Yan Wang,Yi-Jun Fan,Dan Li,Yun-Xia Cao,Lei Zhan

Frontiers in cell and developmental biology 9:669696 PubMed34095138

2021

The Regulatory NOD-Like Receptor NLRC5 Promotes Ganglion Cell Death in Ischemic Retinopathy by Inducing Microglial Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Deng,Yunzhao Fu,Longxiang Sheng,Yixin Hu,Lishi Su,Jiawen Luo,Chun Yan,Wei Chi

Frontiers in pharmacology 11:1281 PubMed33013364

2020

NLRC5 Inhibits Inflammation of Secretory Phase Ectopic Endometrial Stromal Cells by Up-Regulating Autophagy in Ovarian Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Runhua He,Xiaojing Liu,Jing Zhang,Zhongzheng Wang,Wenyan Wang,Liutao Fu,Yijun Fan,Shiying Sun,Yunxia Cao,Lei Zhan,Lijun Shui

The Journal of international medical research 48:300060520940455 PubMed32790491

2020

NLRC5 alleviated OGD/R-induced PC12-cell injury by inhibiting activation of the TLR4/MyD88/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Yuhan Sun,Xin Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLRC5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLRC5 antibody (AB105411)

Western blot - Anti-NLRC5 antibody (AB105411)

Western blot - Anti-NLRC5 antibody (AB105411)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise