Goat Polyclonal NLRP3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 133 publications.
Images
Publications
Filter by
- Aging 15:12738-127482023Contribution of membrane raft redox signalling to visfatin-induced inflammasome activation and podocyte injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaisudha Koka,Sreenidhi Surineni,Gurinder Bir Singh,Krishna M BoiniPubMed 38032896
- Nature communications 14:72462023Cryo-electron tomography of NLRP3-activated ASC complexes reveals organelle co-localization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangci Liu,Haoming Zhai,Helen Alemayehu,Jérôme Boulanger,Lee J Hopkins,Alicia C Borgeaud,Christina Heroven,Jonathan D Howe,Kendra E Leigh,Clare E Bryant,Yorgo ModisPubMed 37945612
- The Journal of clinical investigation 133:2023PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Baldrighi,Christian Doreth,Yang Li,Xiaohui Zhao,Emily Warner,Hannah Chenoweth,Kamal Kishore,Yagnesh Umrania,David-Paul Minde,Sarah Thome,Xian Yu,Yuning Lu,Alice Knapton,James Harrison,Murray Clarke,Eicke Latz,Guillermo de Cárcer,Marcos Malumbres,Bernhard Ryffel,Clare Bryant,Jinping Liu,Kathryn S Lilley,Ziad Mallat,Xuan LiPubMed 37698938
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition 54:965-9712023[Photobiomodulation Promotes Hippocampal Neurogenesis and Improves Cognitive Function and Anti-Inflammatory Injury in Rats With Chronic Cerebral Hypoperfusion].Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyi Tu,Yuanyuan Huang,Ying Huang,Meng Wu,Ruimin WangPubMed 37866954
- Clinical and translational medicine 13:e14052023METTL3-mediated m A modification of circPRKAR1B promotes Crohn's colitis by inducing pyroptosis via autophagy inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhao,Zhibin Zhao,Pu Ying,Yan Zhou,Ziwei Xu,Honggang Wang,Liming TangPubMed 37679886
- Frontiers in endocrinology 14:12026862023Weight bearing training alleviates muscle atrophy and pyroptosis of middle-aged rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengyu Fu,Lijing Gong,Luyao Yang,Shuning Tang,Fangyuan MaPubMed 37720530
- Cellular & molecular biology letters 28:662023MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Martino,Nunzia D'Onofrio,Anna Balestrieri,Luigi Mele,Celestino Sardu,Raffaele Marfella,Giuseppe Campanile,Maria Luisa BalestrieriPubMed 37587410
- Phytotherapy research : PTR 37:4771-47902023NPLC0393 from Gynostemma pentaphyllum ameliorates Alzheimer's disease-like pathology in mice by targeting protein phosphatase magnesium-dependent 1A phosphatase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianlu Lv,Xingyi Shen,Xinya Shen,Shimei Zhao,Rui Xu,Qiuying Yan,Jian Lu,Danyang Zhu,Yonghua Zhao,Jiajia Dong,Jiaying Wang,Xu ShenPubMed 37434441
- Immunity, inflammation and disease 11:e8292023Myo-inositol oxygenase (MIOX) accelerated inflammation in the model of infection-induced cardiac dysfunction by NLRP3 inflammasome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Zhou,Congyi Yu,Yiwen LongPubMed 37249295
- Iranian journal of basic medical sciences 26:579-5862023Effects of three types of fresh improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Liu,Mengnan Zeng,Pengli Guo,Yuhan Zhang,Xiaofeng Yang,Jufang Jia,Qinqin Zhang,Beibei Zhang,Bing Cao,Ru Wang,Xiaoke Zheng,Weisheng FengPubMed 37051100
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q96P20
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Rat | Predicted | Not recommended | Predicted |
| Cow | Predicted | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Cow | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed:16407889, PubMed:18403674, PubMed:18604214, PubMed:23582325, PubMed:25686105, PubMed:27929086, PubMed:28656979, PubMed:28847925, PubMed:30487600, PubMed:30612879, PubMed:31086327, PubMed:31086329, PubMed:31189953, PubMed:33231615, PubMed:34133077, PubMed:34341353, PubMed:34512673, PubMed:36442502). In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed:16407889, PubMed:18403674, PubMed:27432880, PubMed:28847925, PubMed:31189953, PubMed:33231615, PubMed:34133077, PubMed:34341353, PubMed:36142182, PubMed:36442502). Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed:23582325, PubMed:28847925, PubMed:31189953, PubMed:33231615, PubMed:34133077, PubMed:34341353). Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed:22801494). Under resting conditions, ADP-bound NLRP3 is autoinhibited (PubMed:35114687). NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed:16407889, PubMed:18403674, PubMed:18604214, PubMed:19414800, PubMed:23871209). Almost all stimuli trigger intracellular K(+) efflux (By similarity). These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity). Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed:36442502). Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed:30487600, PubMed:34554188). Shows ATPase activity (PubMed:17483456). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription (By similarity). Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3' (By similarity). May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).
Alternative names
C1orf7, CIAS1, NALP3, PYPAF1, NLRP3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, CLR1.1
Recommended products
Goat Polyclonal NLRP3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 133 publications.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q96P20
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
No signal has been obtained in Western blot but low background has observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1μg/ml. We would appreciate any feedback from people in the field.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NLRP3 also known as NALP3 or cryopyrin is a protein important for the inflammatory response. It is a component of the inflammasome which senses pathogenic microorganisms and stress. NLRP3 weighs around 118 kDa and is widely expressed in innate immune cells particularly macrophages and dendritic cells. Researchers often use techniques such as 'NLRP3 WB' (Western blot) and 'NLRP3 immunofluorescence' to study NLRP3 expression and localization.
- Biological function summary
NLRP3 is central to the body’s defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1β and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.
- Pathways
The NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.
- Associated diseases and disorders
NLRP3 has a significant role in inflammatory diseases and is linked to conditions like cryopyrin-associated periodic syndromes (CAPS) and gout. Mutations or dysregulation of NLRP3 lead to hyperactivation resulting in autoimmune and autoinflammatory conditions. NLRP3's interaction with IL-1 contributes to the excessive inflammatory response seen in these disorders. Understanding NLRP3's function in these pathways can inform therapeutic approaches targeting chronic inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-NLRP3 antibody (ab4207)
False colour image of Western blot: Anti-NLRP3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab4207 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line Human NLRP3 knockout THP-1 cell line ab280063 (knockout cell lysate Human NLRP3 knockout THP-1 cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Donkey anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216778) at 1/20000 dilution.
All lanes: Western blot - Anti-NLRP3 antibody (ab4207) at 1/1000 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: NLRP3 knockout THP-1 cell lysate at 20 µg
Lane 2: Western blot - Human NLRP3 knockout THP-1 cell line (Human NLRP3 knockout THP-1 cell line ab280063)
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab4207 was 1hr (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
This image is courtesy of a customer review submitted by Mahesh Shivananjappa.Flow Cytometry (Intracellular) - Anti-NLRP3 antibody (ab4207)
ab4207 staining NLRP3 in the Human White Blood Cells (Mixed Population) by Flow Cytometry. WBC were isolated spinning Blood on Ficoll Gradient after removal of RBC's and permeabilized with 0.1% Triton-X100 in 2% BSA for 15 minutes. The sample was incubated with the primary antibody (1/100 in PBS + 2% BSA in PBS) for 16 hours at 4°C. An Alexa Flour® 488 Donkey Anti Goat IgG (H+L) (1/250) was used as the secondary antibody.
Gating Strategy: Monocytes
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com