AB270449

Anti-NLRP3 antibody [EPR23073-96]

Name: Anti-NLRP3 antibody [EPR23073-96] (ab270449) | Abcam
Brand: Abcam Limited
SKU: AB270449
Price: 245 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(75 Publications)

Anti-NLRP3 antibody [EPR23073-96] (ab270449) is a rabbit monoclonal antibody detecting NLRP3 in Western Blot, IP, ICC/IF. Suitable for Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

Cias1, Mmig1, Nalp3, Pypaf1, Nlrp3, Cold autoinflammatory syndrome 1 protein homolog, Cryopyrin, Mast cell maturation-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (untreated)(-) and RAW 264.7 (+LPS 10ug/ml 8h)(+) cells labelling NLRP3 with ab270449 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 after treatment with LPS. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.

Unknown

Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

NLRP3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) (treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours) whole cell lysate with ab270449 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270449 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : RAW 264.7 treated with 10 ug/ml Lipopolysaccharides (LPS) for 8 hours, whole cell lysate 10 ug

Lane 2 : ab270449 IP in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab270449 in RAW 264.7 treated with 10 ug/ml Lipopolysaccharides(LPS) for 8 hours, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds.

The band around 75kDa corresponds to the short form of NLRP3.

All lanes:

Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] (ab270449)

Predicted band size: 118 kDa

Observed band size: 118 kDa

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Collaborator

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution

Lane 1:

Wild-type LPS treated (3 hour, 50 ng/mL) Mouse BMDCs at 25000 Cells

Lane 2:

NLRP3 knockout LPS treated (3 hour, 50 ng/mL) Mouse BMDCs at 25000 Cells

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) (HRP) at 1/5000 dilution

Predicted band size: 118 kDa

true

Exposure time: 2min

This image is courtesy of Benedikt Saller, from Prof. Dr. Olaf Groß’ lab at the University of Freiburg

Supplier Data

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Lane1-8 : 180 seconds

NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution

Lane 1:

Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

Mouse thymus tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse placenta tissue lysate at 20 µg

Lane 8:

Mouse ovary tissue lysate at 20 µg

Lane 9:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Unknown

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight/expression profile observed is consistent with what has been described in the literature (PMID : 26939933, 30315268 ).

The band around 75kDa corresponds to the short form of NLRP3.

Exposure time : 54 seconds.

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23073-96] (ab270449) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 treated with 10 μg/ml Lipopolysaccharides (LPS) for 8 hours whole cell lysate at 20 µg

Lane 3:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

CiteAb

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

NLRP3 western blot using anti-NLRP3 antibody [EPR23073-96] ab270449. Publication image and figure legend from Wang, Y., Jing, L., et al., 2023, Stem Cell Res Ther, PubMed 37726853.

ab270449 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab270449 please see the product overview.

UCMSC-derived ApoEVs inhibited macrophage pyroptosis present during skin defect healing in db/db mice. a Immunofluorescence staining shows UCMSC-derived ApoEVs phagocytosed by F4/80-positive macrophages in vivo. Scale bar, 100 μm in low-magnification images, 20 μm in high-magnification images. b Tissue inflammatory factors IL-1β and IL-18 of different groups released in and around mouse skin defect area detected by ELISA, N = 3 per group, 3 duplication per sample. c Tissue protein levels of NLRP3, caspase-1, cleaved caspase-1, gasdermin D, cleaved gasdermin D of different groups released in and around mouse skin defect area detected by Western Blots, N = 3 per group. Corresponding uncropped full-length gels and blots are presented in Additional file 5B. d Semi-quantification of protein expression of NLRP3, cleaved caspase-1, and cleaved gasdermin D. e Immunofluorescence staining shows NLRP3, cleaved caspase-1, and gasdermin D in the skin defect area of mice in all three groups had significant co-localization with F4/80-positive macrophages. Scale bar, 40 μm in low-magnification images, 20 μm in high-magnification images. N = 3 per group, 3 ROIs per sample. f Semi-quantification of NLRP3, cleaved caspase-1, and gasdermin D relative fluorescence intensity in F4/80-positive cells of skin defect area. Data shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; NS not significant. CASP1 caspase-1; GSDMD, gasdermin D

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Carrier free

Anti-NLRP3 antibody [EPR23073-96] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23073-96

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

NLRP3 is related with inflammation and is cell-type dependent. This antibody detects no signal or very weak signal in most normal natural samples. Stimulation may be required to allow detection of the NLRP3 protein in most samples, as described in the literature (PMID: 29070054, 29434227, and 22569257).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Stimulation may be required to allow detection of the NLRP3 protein in most samples.There may be multiple bands of NLRP3 in WB assay due to different isoforms and PTMs.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-NLRP3 antibody [EPR23073-96] (ab270449) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of NLRP3?
Anti-NLRP3 [EPR23073-96] (ab270449) specifically detects a band for NLRP3 (UniProt: Q8R4B8) at a molecular weight of 118kDa.

Trusted by the scientific community
Anti-NLRP3 [EPR23073-96] (ab270449) was first used in a scientific publication in 2020 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-NLRP3 antibody [EPR23073-96] (ab270449) has been confirmed by Western blot testing in Nlrp3 Knockout Mouse BMDCs.

Other related products
We have a range of other formats of antibody clone [EPR23073-96] also available for your convenience: ab270449, Carrier free - ab272702

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLRP3 also known as NALP3 or cryopyrin is a protein important for the inflammatory response. It is a component of the inflammasome which senses pathogenic microorganisms and stress. NLRP3 weighs around 118 kDa and is widely expressed in innate immune cells particularly macrophages and dendritic cells. Researchers often use techniques such as 'NLRP3 WB' (Western blot) and 'NLRP3 immunofluorescence' to study NLRP3 expression and localization.

Biological function summary

NLRP3 is central to the body's defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1Β and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.

Pathways

The NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.

NLRP3 has a significant role in inflammatory diseases and is linked to conditions like cryopyrin-associated periodic syndromes (CAPS) and gout. Mutations or dysregulation of NLRP3 lead to hyperactivation resulting in autoimmune and autoinflammatory conditions. NLRP3's interaction with IL-1 contributes to the excessive inflammatory response seen in these disorders. Understanding NLRP3's function in these pathways can inform therapeutic approaches targeting chronic inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed : 19362020, PubMed : 23582325, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 27929086, PubMed : 28656979, PubMed : 28847925, PubMed : 30518920, PubMed : 36178239). In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed : 16407889, PubMed : 18403674, PubMed : 19362020, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed : 16546100, PubMed : 17008311, PubMed : 26642356, PubMed : 26814970, PubMed : 27374331, PubMed : 28847925). Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed : 22801494). Involved in the homeostatic wound healing response to tissue injury, a multistep cascade that guides neutrophil migration to necrotic sites while avoiding collateral damage of healthy tissues. ATP released from necrotic cells triggers activation of NLRP3 inflammasome through P2RX7 signaling leading to neutrophil adhesion to the vascular endothelium close to the injury site (PubMed : 20947763). Under resting conditions, ADP-bound NLRP3 is autoinhibited (By similarity). NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed : 16407888, PubMed : 16407889, PubMed : 16407890, PubMed : 18403674, PubMed : 19362020, PubMed : 37001519). Almost all stimuli trigger intracellular K(+) efflux (PubMed : 23809161). These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity). Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed : 26814970, PubMed : 34615873, PubMed : 34861190). Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed : 30487600). Shows ATPase activity (PubMed : 34861190).. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (PubMed : 26098997). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription (PubMed : 26098997). Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3' (PubMed : 26098997). May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed : 26098997).

See full target information Nlrp3

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Publications (75)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1420 PubMed41044169

2025

T3SS effector EseJ in Edwardsiella piscicida inhibits PANoptosis in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian He,Pu Yu Tang,Xiu Long Jiang,Shan Shan Sun,Pin Nie,Hai Xia Xie

Journal of biomedical research :1-15 PubMed40878776

2025

hUMSC-derived exosomes alleviate hypoxic cerebrovascular injury AMPK/NLRP3-mediated pyroptosis suppression and mitochondrial protection.

Applications

Unspecified application

Species

Unspecified reactive species

Jinteng Liu,Yunlong Pan,Haolin Wu,Qingyun Guo,Xingyue Fang,Yingmei Lu,Qibing Liu

Journal of inflammation research 18:10425-10443 PubMed40787259

2025

Qingwen Hufei Granules Attenuate Lipopolysaccharide-Induced Acute Lung Injury by Suppressing NLRP3 Inflammasome-Dependent Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaihua Long,Yun Yang,Yuan Wang,Bo Wang,Huiying Zhou,Yuxi Liu,Ye Li,Shuanzhu Yang,Liping Cao,Tingting Huang,Yang Liu,Hong Zhang

International journal of molecular medicine 56: PubMed40613239

2025

Benzo[a]pyrene aggravated ovalbumin‑induced epithelial tight junction disruption via ROS driven‑NLRP3/Caspase‑1 signaling pathway in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqiu Xu,Yanming Feng,Ling Wang,Xin Xu,Li Xu,Bohan Wang

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:2189-2202 PubMed40569616

2025

Assessing the effects of aging on the Koizumi's and Zea Longa's methods and their suitability as mouse models for evaluating neurodegeneration post-ischemic stroke: A comparative study.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyeon Lim,Hongrae Kim,Sehyun Lim,Hyo-Eun Kim,So-Jung Moon,Hyung-Hwan Kim,Suin Cho

Probiotics and antimicrobial proteins : PubMed40526309

2025

Limosilactobacillus fermentum 21 N1 Mitigates Candida albicans-Induced Vulvovaginal Candidiasis and Suppresses NLRP3 Inflammasome Activation in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Yeong Lim,Gun-Dong Kim,Geun-Hye Hong,So-Young Lee,So-Young Lee,Hee Soon Shin

Psychopharmacology : PubMed40493075

2025

Ginsenoside Rg1 alleviates PCPA-induced insomnia by inhibiting NLRP3 inflammasome activation and pyroptosis through the Nrf2/HO-1 pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Xiufeng Wang,Yu Zhang,Min Wei,Jianqiang Qi,Dan Liu,Runhua Wu,Qin Chen,Junshan Huang

Frontiers in pharmacology 16:1590096 PubMed40406492

2025

Rutin ameliorates LPS-induced acute lung injury in mice by inhibiting the cGAS-STING-NLRP3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Zhibin Wang,Yuting Wang,Guofeng Xu,Min Luo,Mengwei Zhang,Yuying Li

Psychopharmacology 242:1797-1809 PubMed40237808

2025

MiR-223-3p inhibits hippocampal neurons injury and exerts anti- anxiety/depression-like behaviors by directly targeting NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyuan Wang,Tingting Yang,Na Liu,Lin Yang,Cong Liu,Xiaoxiao Qi,Ning Wang,Mingwei Wang,Yanyong Wang

Cell research 35:437-452 PubMed40164768

2025

Signal-induced NLRP3 phase separation initiates inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gonglu Zou,Yuluan Tang,Jie Yang,Shuo Fu,Yuheng Li,Xuanyao Ren,Nanhai Zhou,Wenlong Zhao,Juyi Gao,Ziran Ruan,Zhengfan Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NLRP3 antibody [EPR23073-96]

Immunocytochemistry/ Immunofluorescence - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Immunoprecipitation - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Western blot - Anti-NLRP3 antibody [EPR23073-96] (AB270449)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (75)

Product promise