AB264468

Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free

KO Validated
RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal NLRP3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IP, I-ELISA and reacts with Human, Mouse, Rat, Recombinant full length protein samples. Cited in 9 publications.

View Alternative Names

C1orf7, CIAS1, NALP3, PYPAF1, NLRP3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1, CLR1.1

12 Images

Flow Cytometry (Intracellular) - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labeling NLRP3 with ab263899 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

Unknown

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

NLRP3 was immunoprecipitated from 0.35 mg THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10μg with ab263899 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263899 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : THP-1 whole cell lysate 10μg

Lane 2 : ab263899 IP in THP-1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab263899 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

All lanes:

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>)

Predicted band size: 118 kDa

false

Unknown

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

NLRP3 was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate 10μg with ab263899 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263899 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : RAW 264.7 whole cell lysate 10μg

Lane 2 : ab263899 IP in RAW 264.7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab263899 in RAW 264.7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

All lanes:

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>)

Predicted band size: 118 kDa

false

Lab

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

The molecular weight/expression profile observed is consistent with what has been described in the literature (PMID : 26939933, 30315268).

Note : The band around 75kDa is the short form of NLRP3 (NLRP3s).

Low expression cell line : Jurkat (PMID : 19767079).

Exposure times : Lane 1 : 48 secs; Lane 2 : 3 min; Lanes 3-4 : 1 sec; Lane 5 : 6 secs.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

Lane 3:

Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 4:

RAW 264.7 treated with 10 μg/ml LPS for 8 hours, whole cell lysate at 10 µg

Lane 5:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 118 kDa

false

Lab

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

This data was developed using ab263899, the same antibody clone in a different buffer formulation.

ab263899 was shown to react with NLRP3 in wild-type THP-1 cells in Western blot with loss of signal observed in a NLRP3 knockout cell line. Wild-type THP-1 and NLRP3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab263899 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

Wild-type THP-1 lysate at 20 µg

Lane 2:

Wild-type THP-1 lysate treated with 200 ng/ml PMA, 48 hr at 20 µg

Lane 3:

NLRP3 knock-out THP-1 lysate at 20 µg

Lane 4:

NLRP3 knock-out THP-1 lysate treated with 200 ng/ml PMA, 48 hr at 20 µg

false

Lab

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

False colour image of Western blot : Anti-NLRP3 antibody [EPR23094-1] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263899 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) at 1/20000 dilution.

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/500 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

NLRP3 knockout THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human NLRP3 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-nlrp3-knockout-thp-1-cell-line-ab280063'>ab280063</a>)

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Collaborator

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Blocking step : 2% SMP in PBS/T for 60 minutes at RT.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse BMDCs treated with 50 ng/ml LPS at 25000 Cells

Lane 2:

NLRP3 knockout mouse BMDCs treated with 50 ng/ml LPS at 25000 Cells

Lane 3:

Wild-type human THP1 cells treated with 200 ng/ml LPS at 25000 Cells

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/5000 dilution

Predicted band size: 118 kDa

true

Exposure time: 10s

This image is courtesy of Benedikt Saller, from Prof. Dr. Olaf Groß’ lab at the University of Freiburg.

Supplier Data

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899). Blocking and Diluting buffer : 5% NFDM/TBST Lane 9 Exposure Time : 20 seconds NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

Mouse thymus tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

Mouse placenta tissue lysate at 20 µg

Lane 7:

Mouse ovary tissue lysate at 20 µg

Lane 8:

Mouse liver tissue lysate at 20 µg

Lane 9:

Raw 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

The molecular weight/expression profile observed is consistent with what has been described in the literature (PMID : 26939933, 30315268).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263899).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

All lanes:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 118 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Blocking and dilution buffer : 5% NFDM /TBST. NLRP3 is expressed at a much low level in normal tissues (PMID : 33538177, PMID : 25524927, PMID : 20688930, PMID : 35676979, PMID : 31923221, PMID : 24166187).

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

Rat spleen tissue lysate at 20 µg

Lane 2:

Rat thymus tissue lysate at 20 µg

Lane 3:

Rat lung tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat hippocampus tissue lysate at 20 µg

Lane 8:

Rat spinal cord tissue lysate at 20 µg

Lane 9:

Rat placenta tissue lysate at 20 µg

Lane 10:

Rat ovary tissue lysate at 20 µg

Lane 11:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 180s

Indirect ELISA - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

I-ELISA

Unknown

Indirect ELISA - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

This data was developed using ab263899, the same antibody clone in a different buffer formulation.ELISA analysis of NLRP3 recombinant protein at 1000 ng/mL with ab263899. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Unknown

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

All lanes:

Western blot - Anti-NLRP3 antibody [EPR23094-1] (<a href='/en-us/products/primary-antibodies/nlrp3-antibody-epr23094-1-ab263899'>ab263899</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Exposure time: 15s

Unconjugated

Anti-NLRP3 antibody [EPR23094-1]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23094-1

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, I-ELISA, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

NLRP3 is related with inflammation and is cell-type dependent. This antibody detects no signal or very weak signal in most non-diseased tissue samples and untreated cell lysates. Stimulation may be required to allow detection of the NLRP3 protein in most samples, as described in the literature (PMID: 29070054, 29434227, and 22569257).

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.

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Reactivity data

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Product details

ab264468 is the carrier-free version of ab263899.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLRP3 also known as NALP3 or cryopyrin is a protein important for the inflammatory response. It is a component of the inflammasome which senses pathogenic microorganisms and stress. NLRP3 weighs around 118 kDa and is widely expressed in innate immune cells particularly macrophages and dendritic cells. Researchers often use techniques such as 'NLRP3 WB' (Western blot) and 'NLRP3 immunofluorescence' to study NLRP3 expression and localization.

Biological function summary

NLRP3 is central to the body’s defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1? and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.

Pathways

The NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.

NLRP3 has a significant role in inflammatory diseases and is linked to conditions like cryopyrin-associated periodic syndromes (CAPS) and gout. Mutations or dysregulation of NLRP3 lead to hyperactivation resulting in autoimmune and autoinflammatory conditions. NLRP3's interaction with IL-1 contributes to the excessive inflammatory response seen in these disorders. Understanding NLRP3's function in these pathways can inform therapeutic approaches targeting chronic inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis (PubMed : 16407889, PubMed : 18403674, PubMed : 18604214, PubMed : 23582325, PubMed : 25686105, PubMed : 27929086, PubMed : 28656979, PubMed : 28847925, PubMed : 30487600, PubMed : 30612879, PubMed : 31086327, PubMed : 31086329, PubMed : 31189953, PubMed : 33231615, PubMed : 34133077, PubMed : 34341353, PubMed : 34512673, PubMed : 36442502). In response to pathogens and other damage-associated signals that affect the integrity of membranes, initiates the formation of the inflammasome polymeric complex composed of NLRP3, CASP1 and PYCARD/ASC (PubMed : 16407889, PubMed : 18403674, PubMed : 27432880, PubMed : 28847925, PubMed : 31189953, PubMed : 33231615, PubMed : 34133077, PubMed : 34341353, PubMed : 36142182, PubMed : 36442502). Recruitment of pro-caspase-1 (proCASP1) to the NLRP3 inflammasome promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), promoting cytokine secretion and pyroptosis (PubMed : 23582325, PubMed : 28847925, PubMed : 31189953, PubMed : 33231615, PubMed : 34133077, PubMed : 34341353). Activation of NLRP3 inflammasome is also required for HMGB1 secretion; stimulating inflammatory responses (PubMed : 22801494). Under resting conditions, ADP-bound NLRP3 is autoinhibited (PubMed : 35114687). NLRP3 activation stimuli include extracellular ATP, nigericin, reactive oxygen species, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, such as asbestos, silica, aluminum salts, cytosolic dsRNA, etc (PubMed : 16407889, PubMed : 18403674, PubMed : 18604214, PubMed : 19414800, PubMed : 23871209). Almost all stimuli trigger intracellular K(+) efflux (By similarity). These stimuli lead to membrane perturbation and activation of NLRP3 (By similarity). Upon activation, NLRP3 is transported to microtubule organizing center (MTOC), where it is unlocked by NEK7, leading to its relocalization to dispersed trans-Golgi network (dTGN) vesicle membranes and formation of an active inflammasome complex (PubMed : 36442502). Associates with dTGN vesicle membranes by binding to phosphatidylinositol 4-phosphate (PtdIns4P) (PubMed : 30487600, PubMed : 34554188). Shows ATPase activity (PubMed : 17483456).. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription (By similarity). Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3' (By similarity). May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).

See full target information NLRP3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Ecotoxicology and environmental safety 278:116433 PubMed38714087

2024

The role of PANoptosis in renal vascular endothelial cells: Implications for trichloroethylene-induced kidney injury.

Applications

Species

Mouse

Haibo Xie,Bo Liang,Qixing Zhu,Lin Wang,Hui Li,Zhuohui Qin,Jiaxiang Zhang,Zhibing Liu,Yonggui Wu

Cardiology research 15:108-116 PubMed38645829

2024

Increased Cyclic Guanosine Monophosphate and Interleukin-1Beta Is Activated by Mitochondrial Dysfunction and Associated With Heart Failure in Atrial Fibrillation Patients.

Applications

Species

Rat

Juledezi Hailati,Zhi Qiang Liu,Yun Fei Zhang,Lei Zhang,Hasidaer Midilibieke,Xiang Li Ma,Muhuyati Wulasihan

Annals of translational medicine 10:1376 PubMed36660616

2023

M2 macrophage-derived exosomal miR-145-5p protects against the hypoxia/reoxygenation-induced pyroptosis of cardiomyocytes by inhibiting TLR4 expression.

Applications

Species

Human

Li Wei,Dongsheng Zhao

Cell biology international 47:394-405 PubMed36525374

2022

USP9X promotes lipopolysaccharide-stimulated acute lung injury by deubiquitination of NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Yijin Xiang,Xiangting Li,Min Cai,Dingfang Cai

Translational research : the journal of laboratory and clinical medicine 254:115-127 PubMed36336332

2022

Disulfiram accelerates diabetic foot ulcer healing by blocking NET formation via suppressing the NLRP3/Caspase-1/GSDMD pathway.

Applications

WB, IHC-P, IHC-P, WB

Species

Mouse, Mouse, Human, Human

Shuofei Yang,Yu Feng,Liang Chen,Zheyu Wang,Jiaquan Chen,Qihong Ni,Xiangjiang Guo,Lan Zhang,Guanhua Xue

Toxicology research 11:437-450 PubMed35782648

2022

Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huayu Li,Mengyan Li,Chao Dong,Bing Liu

Mediators of inflammation 2022:5515305 PubMed35399795

2022

Role of NOD-Like Receptors in a Miniature Pig Model of Diabetic Renal Injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ren,Shaoyuan Cui,Quan Hong,Wanjun Shen,Qinggang Li,Lingling Wu,Bo Fu,Xu Wang,Qian Ma,Jiaona Liu,Xueyuan Bai,Xiangmei Chen

Experimental cell research 410:112967 PubMed34883112

2021

MF-094, a potent and selective USP30 inhibitor, accelerates diabetic wound healing by inhibiting the NLRP3 inflammasome.

Applications

Species

Human

Xu Li,Tao Wang,Yue Tao,Xiaojun Wang,Limeng Li,Jianjun Liu

PloS one 15:e0234039 PubMed32555710

2020

NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture.

Applications

Unspecified application

Species

Unspecified reactive species

Denise C Cornelius,Olivia K Travis,Robert W Tramel,Marivee Borges-Rodriguez,Cedar H Baik,Mallory Greer,Chelsea A Giachelli,Geilda A Tardo,Jan M Williams

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Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Immunoprecipitation - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Indirect ELISA - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Western blot - Anti-NLRP3 antibody [EPR23094-1] - BSA and Azide free (AB264468)

Related conjugates and formulations (1)

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Carrier free

Reacts with

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Publications (9)

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