Anti-NLRP6 antibody [EPR28233-48] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-NLRP6 antibody [EPR28233-48] - BSA and Azide free (AB314499)

This data was developed usingab314498, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen(PMID : 21543645) Lane1 was freshly made and used for Western blotting immediately to minimize protein degradation. The bands beneath the target band (98 kDa) are expected to be degradation products (PMID : 33376129), with the exception of the band around 50kd. The identity of the bands around 50 kDa are unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-NLRP6 antibody [EPR28233-48] (<a href='/en-us/products/primary-antibodies/nlrp6-antibody-epr28233-48-ab314498'>ab314498</a>) at 1/1000 dilution

Lane 1:

Rat colon tissue lysate at 20 µg

Lane 2:

Rat small intestine tissue lysate at 20 µg

Lane 3:

Rat colon tissue lysate 20 µg at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-NLRP6 antibody [EPR28233-48] - BSA and Azide free (AB314499)

This data was developed using ab314498, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : stomach, spleen(PMID : 21543645) Lane1 was freshly made and used for Western blotting immediately to minimize protein degradation. The bands beneath the target band (98 kDa) are expected to be degradation products (PMID : 33376129), with the exception of the band around 50kd. The identity of the bands around 50 kDa are unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. Exposure time : Lane 1 : 70 seconds, lane 2-6 : 180 seconds

All lanes:

Western blot - Anti-NLRP6 antibody [EPR28233-48] (<a href='/en-us/products/primary-antibodies/nlrp6-antibody-epr28233-48-ab314498'>ab314498</a>) at 1/1000 dilution

Lanes 1 - 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse colon cancer tissue lysate at 20 µg

Lane 4:

Mouse small intestine tissue lysate at 20 µg

Lane 5:

Mouse stomach tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 98 kDa,37 kDa

false

• WB

Supplier Data

Western blot - Anti-NLRP6 antibody [EPR28233-48] - BSA and Azide free (AB314499)

This data was developed usingab314498, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution

All lanes:

Western blot - Anti-NLRP6 antibody [EPR28233-48] (<a href='/en-us/products/primary-antibodies/nlrp6-antibody-epr28233-48-ab314498'>ab314498</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human NLRP6 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 98 kDa,37 kDa,80 kDa

false

Exposure time: 3s