AB105413

Anti-NLRP9 antibody

Name: Anti-NLRP9 antibody (ab105413) | Abcam
Brand: Abcam Limited
SKU: AB105413

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(2 Publications)

Rabbit Polyclonal NLRP9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NLRP9.

View Alternative Names

NALP9, NOD6, PAN12, NLRP9, Nucleotide-binding oligomerization domain protein 6, PYRIN and NACHT-containing protein 12

1 Images

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Western blot - Anti-NLRP9 antibody (AB105413)

All lanes:

Western blot - Anti-NLRP9 antibody (ab105413) at 1 µg/mL

Lane 1:

EL4 cell lysate

Lane 2:

EL4 cell lysate with blocking Peptide

Predicted band size: 113 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human NLRP9. The exact immunogen used to generate this antibody is proprietary information.

Q7RTR0

Specificity

At least two isoforms of NLRP9 are known to exist; ab105413 will recognize both. This antibody is predicted to not crossreact with other NLRP family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NLRP9 also known as Nacht LRR and PYD domains-containing protein 9 is a member of the NLR family which plays a significant role in the immune system. The protein has a molecular mass of approximately 113 kDa and is expressed in several tissues including the ovary and testes. Structurally NLRP9 contains three main domains: the NOD (nucleotide-binding and oligomerization domain) LRR (leucine-rich repeat) and PYD (pyrin domain) which contribute to its function in inflammasome formation.

Biological function summary

NLRP9 participates in the regulation of inflammation and immune responses. It acts as part of the inflammasome complex a multiprotein oligomer responsible for activating inflammatory responses. Though less studied than other inflammasomes like NLRP3 studies suggest NLRP9 interacts with caspase-1 and ASC (apoptosis-associated speck-like protein containing a CARD) facilitating the processing of pro-inflammatory cytokines like IL-1β and IL-18. Such interactions emphasize its role in maintaining the balance of immune responses.

Pathways

NLRP9 influences immune pathways by contributing to the regulation of inflammasomes. NLRP9 is notably involved in the inflammatory signaling pathway. This involvement includes interaction with other NLR family proteins such as NLRP3 under certain physiological conditions. Connections to these pathways highlight its role in innate immunity and highlight its importance in preparing the body's defense mechanisms.

NLRP9's function becomes critical in situations such as viral infections and certain types of infertility. Altered expression or malfunction of NLRP9 has associations with disorders where immune regulation is disrupted. For example excessive or inadequate activity relates to improper inflammatory responses during viral infections. NLRP9's interaction with proteins like caspase-1 in these contexts can either mitigate or exacerbate disease symptoms depending on the underlying biological circumstances.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

As the sensor component of the NLRP9 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens, including rotavirus, initiates the formation of the inflammasome polymeric complex, made of NLRP9, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and release in the extracellular milieu. The active cytokines stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. NLRP9 inflammasome activation may be initiated by DHX9 interaction with viral double-stranded RNA (dsRNA), preferentially to short dsRNA segments.

See full target information NLRP9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:891 PubMed36270983

2022

NLRP6 is required for cancer-derived exosome-modified macrophage M2 polarization and promotes metastasis in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinrui Rao,Xiaoshu Zhou,Geng Wang,Xiaohua Jie,Biyuan Xing,Yingzhuo Xu,Yunshang Chen,Jun Li,Kuikui Zhu,Zilong Wu,Gang Wu,Chuangyan Wu,Rui Zhou

Aging 13:8127-8145 PubMed33686956

2021

Long noncoding RNA NKILA transferred by astrocyte-derived extracellular vesicles protects against neuronal injury by upregulating NLRX1 through binding to mir-195 in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bin He,Wei Chen,Jingsong Zeng,Wusong Tong,Ping Zheng

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NLRP9 antibody

Western blot - Anti-NLRP9 antibody (AB105413)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise