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AB154547

Anti-NM23A antibody

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(4 Publications)

Rabbit Polyclonal NM23A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NME1 aa 1-200.

View Alternative Names

NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, NDP kinase A, NDPK A, Farnesyl diphosphate kinase NME1, Histidine protein kinase NME1, Metastasis inhibition factor nm23, NM23-H1, Non-specific serine/threonine protein kinase NME1, Nucleoside diphoshate kinase 1, Putative 3'-5'-DNA exonuclease NDK1, Putative granzyme A-activated DNA endonuclease, Tumor metastatic process-associated protein, NDK1, GAAD

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NM23A antibody (AB154547)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NM23A antibody (AB154547)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling NM23A with ab154547 at 1/100 dilution. Lower panel merged with DNA probe.

Western blot - Anti-NM23A antibody (AB154547)
  • WB

Unknown

Western blot - Anti-NM23A antibody (AB154547)

12% SDS PAGE

All lanes:

Western blot - Anti-NM23A antibody (ab154547) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 17 kDa

false

Western blot - Anti-NM23A antibody (AB154547)
  • WB

Unknown

Western blot - Anti-NM23A antibody (AB154547)

12% SDS PAGE

All lanes:

Western blot - Anti-NM23A antibody (ab154547) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 17 kDa,18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NME1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P15531

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NM23A also known as NME1 (non-metastatic cells 1 protein) is a nucleoside diphosphate kinase A. NM23A weighs approximately 17 kDa. This protein is expressed in a variety of tissues with high levels in liver brain and endocrine tissues. By exchanging phosphate groups NM23A is involved in energy transfer in cells. It stabilizes proteins by forming hexameric complexes which are pivotal for its function.
Biological function summary

The NME1 protein plays a significant role in cell proliferation and differentiation. It acts as a marker for the metastatic potential of some tumors where lower expression links to increased metastatic abilities in tumor cells. This protein sometimes works as part of a larger multiprotein complex which influences its functions in cellular processes. It is known for modulating microtubule assembly which affects cell structure and mechanics.

Pathways

NM23A is an important player in signal transduction and DNA repair pathways. It is essential in the regulation of the Ras-MAPK pathway which controls cell growth and differentiation. NM23A interacts with key regulatory proteins such as c-Myc and APC to uphold cellular activities. The protein's influence in these pathways indicates its role in sustaining normal cellular functions and responses.

NM23A relates closely to cancer particularly in metastatic processes and tumor progression. Lower levels of NM23A are associated with the progression of neuroblastoma and breast cancer. In breast cancer NM23A links to the tumor suppressor gene p53 affecting its regulatory functions. Research continues into how NM23A can serve as a useful biomarker for prognosis and therapy in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 10952986, PubMed : 14960567, PubMed : 16313181, PubMed : 1851158, PubMed : 23519676, PubMed : 33903070, PubMed : 8810265, PubMed : 9038158). Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism (PubMed : 10952986). Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins (PubMed : 8529641, PubMed : 9038158). May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes (PubMed : 12628186, PubMed : 16818237). Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair (PubMed : 16818237). Has been shown to cleave double strands DNA within the 3'-portions of both 5'-SHS silencer and NHE basal promoter element of the PDGFA gene, potentially repressing its transcription (PubMed : 11694515). May also function as a Mg(2+)-dependent 3'-5' DNA exonuclease, excising nucleotides from 3' single-stranded DNA or DNA with 3' single strand overhangs, suggesting a role in DNA nucleolytic processing (PubMed : 14960567, PubMed : 16313181). Involved in the regulation of tumor metastasis and cellular differentiation (By similarity). Also required for cell motility (PubMed : 8270257, PubMed : 25582197). May control, with NME2, AcCoA usage between histone acetylation and fatty acid synthesis, possibly by binding and releasing AcCoA at transcriptionally active chromatin regions in proximity to histone acetyltransferase (HAT) (By similarity).
See full target information NME1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neuron 106:76-89.e8 PubMed32004439

2020

Genome-wide In Vivo CNS Screening Identifies Genes that Modify CNS Neuronal Survival and mHTT Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Mary H Wertz,Mollie R Mitchem,S Sebastian Pineda,Lea J Hachigian,Hyeseung Lee,Vanessa Lau,Alex Powers,Ruth Kulicke,Gurrein K Madan,Medina Colic,Martine Therrien,Amanda Vernon,Victoria F Beja-Glasser,Mudra Hegde,Fan Gao,Manolis Kellis,Traver Hart,John G Doench,Myriam Heiman

Frontiers in oncology 9:576 PubMed31312613

2019

Electro-Acupuncture Promotes Accumulation of Paclitaxel by Altering Tumor Microvasculature and Microenvironment in Breast Cancer of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Yang,Yuxiang Wan,Xin Jiang,Xuewei Qi,Lina Wang,Zeyu Liu,Xiaojing Song,Lin Pan,Weiliang Sun,Wei Zhao,Jinchang Huang,Zenglin Lian

Oncology letters 17:1267-1273 PubMed30655894

2019

Inhibition effects of Yuxiao San combined with cisplatin on transplanted tumor growths via upregulation of nm-23 and downregulation of K-ras in Lewis lung cancer mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Tang,Shumei Wang,Yulin Wei,Jianti Fu

Molecular medicine reports 18:3932-3940 PubMed30132534

2018

Downregulation of Krüppel‑like factor 1 inhibits the metastasis and invasion of cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bisheng Zhu,Qisheng Liu,Qi Han,Bohang Zeng,Jingqi Chen,Qiuju Xiao
View all publications
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