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AB171935

Anti-NM23A antibody [EPR10146]

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(2 Publications)

Rabbit Recombinant Monoclonal NM23A antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, GAAD

2 Images
Western blot - Anti-NM23A antibody [EPR10146] (AB171935)
  • WB

Lab

Western blot - Anti-NM23A antibody [EPR10146] (AB171935)

Lanes 1-4 : Merged signal (red and green). Green - ab171935 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.

ab171935 Anti-NM23A antibody [EPR10146] was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab171935 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NM23A antibody [EPR10146] (ab171935) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NME1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NME1 (NM23A) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nme1-nm23a-knockout-hek-293t-cell-line-ab266215'>ab266215</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

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Western blot - Anti-NM23A antibody [EPR10146] (AB171935)
  • WB

Supplier Data

Western blot - Anti-NM23A antibody [EPR10146] (AB171935)

All lanes:

Western blot - Anti-NM23A antibody [EPR10146] (ab171935) at 1/1000 dilution

Lane 1:

HeLa cell line lysate at 10 µg

Lane 2:

MCF7 cell line lysate at 10 µg

Lane 3:

Jurkat cell line lysate at 10 µg

Secondary

All lanes:

Goat ant-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10146

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NM23A also known as NME1 (non-metastatic cells 1 protein) is a nucleoside diphosphate kinase A. NM23A weighs approximately 17 kDa. This protein is expressed in a variety of tissues with high levels in liver brain and endocrine tissues. By exchanging phosphate groups NM23A is involved in energy transfer in cells. It stabilizes proteins by forming hexameric complexes which are pivotal for its function.
Biological function summary

The NME1 protein plays a significant role in cell proliferation and differentiation. It acts as a marker for the metastatic potential of some tumors where lower expression links to increased metastatic abilities in tumor cells. This protein sometimes works as part of a larger multiprotein complex which influences its functions in cellular processes. It is known for modulating microtubule assembly which affects cell structure and mechanics.

Pathways

NM23A is an important player in signal transduction and DNA repair pathways. It is essential in the regulation of the Ras-MAPK pathway which controls cell growth and differentiation. NM23A interacts with key regulatory proteins such as c-Myc and APC to uphold cellular activities. The protein's influence in these pathways indicates its role in sustaining normal cellular functions and responses.

NM23A relates closely to cancer particularly in metastatic processes and tumor progression. Lower levels of NM23A are associated with the progression of neuroblastoma and breast cancer. In breast cancer NM23A links to the tumor suppressor gene p53 affecting its regulatory functions. Research continues into how NM23A can serve as a useful biomarker for prognosis and therapy in cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
See full target information NME1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 18:e1010743 PubMed36067236

2022

Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Yang,Serina Huang,Yasaman Jami-Alahmadi,Gerald M McInerney,James A Wohlschlegel,Melody M H Li

Oncology letters 12:615-620 PubMed27347189

2016

Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance .

Applications

IHC-P

Species

Human

Song Qu,Xiao-Yu Li,Zhong-Guo Liang,Ling Li,Shi-Ting Huang,Jia-Quan Li,Dan-Rong Li,Xiao-Dong Zhu
View all publications
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