AB134141

Anti-NMBR antibody [EPR6880]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal NMBR antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Neuromedin-B receptor, NMB-R, Epididymis tissue protein Li 185a, Neuromedin-B-preferring bombesin receptor, NMBR

2 Images

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Western blot - Anti-NMBR antibody [EPR6880] (AB134141)

All lanes:

Western blot - Anti-NMBR antibody [EPR6880] (ab134141) at 1/10000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

Caco2 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

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OI-RD Scanning - Anti-NMBR antibody [EPR6880] (AB134141)

OI-RD Scanning

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OI-RD Scanning - Anti-NMBR antibody [EPR6880] (AB134141)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-NMBR antibody [EPR6880] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6880

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMBR or Neuromedin B Receptor is a G protein-coupled receptor involved in binding and signaling of the neuropeptide neuromedin B. Frequently called the bombesin receptor subtype-1 (BB1) this receptor has an approximate molecular mass of around 44 kDa. NMBR is widely expressed in peripheral tissues and the central nervous system particularly in the hypothalamus and pituitary gland. Its structure enables it to play a role in signal transduction by activating phospholipase C pathways when bound by its ligand.

Biological function summary

NMBR contributes to various physiological processes including regulation of metabolism and energy balance by controlling appetite and satiety signals. It functions independently without forming part of a larger protein complex. Expression of NMBR in the gastrointestinal tract indicates its potential role in gastrointestinal functioning and smooth muscle contraction. This receptor's interaction with neuromedin B plays an important biological role by modulating these physiological pathways making it an essential component of body regulations such as thermoregulation and stress response.

Pathways

NMBR is significant in the bombesin-related peptide signaling pathways and also in the phosphoinositide pathway associated with energy balance. It interacts closely with bombesin and similar peptide receptors impacting intracellular calcium levels and influencing cellular activities through the PKC pathway (protein kinase C). NMBR's interaction with these pathways shows its role in coordinating complex cellular responses and indicates collaboration with related proteins like neuromedin U receptor for similar regulatory functions.

NMBR has links with conditions such as obesity and lung cancer. In obesity it modulates appetite possibly through interaction with proteins like leptin receptor contributing to energy homeostasis. In lung cancer research indicates that NMBR might influence proliferation of cancer cells in some cases through pathways involving other receptors like GRPR (gastrin-releasing peptide receptor). The receptor's involvement in these diseases highlights its potential as a therapeutic target and points to the broader implications of its regulatory actions in pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for neuromedin-B (PubMed : 1655761). Contributes to the maintenance of basal sigh rate through signaling in the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity (By similarity). Contributes to the induction of sneezing following exposure to chemical irritants or allergens which causes release of NMB by nasal sensory neurons and activation of NMBR-expressing neurons in the sneeze-evoking region of the brainstem (By similarity). These in turn activate neurons of the caudal ventral respiratory group, giving rise to the sneezing response (By similarity). Contributes to induction of acute itch, possibly through its activation on dorsal root ganglion neurons by the NMB peptide (By similarity). Plays a role in the innate immune response to influenza A virus infection by enhancing interferon alpha expression and reducing expression of IL6 (PubMed : 31601264). Plays a role in CSF1-induced proliferation of osteoclast precursors by contributing to the positive regulation of the expression of the CSF1 receptor CSF1R (By similarity).

See full target information Neuromedin-B receptor

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 11:e1071 PubMed38018587

2023

Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Yue,Wei Hao,Min Wang,Yang Fu

Veterinary research 50:80 PubMed31601264

2019

Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Yang,Huipeng Huang,Mengyao Tang,Zifeng Cai,Cuiqin Huang,Baomin Qi,Ji-Long Chen

OncoTargets and therapy 12:7437-7450 PubMed31686849

2019

Ultrasound-microbubbles-mediated microRNA-449a inhibits lung cancer cell growth via the regulation of Notch1.

Applications

Unspecified application

Species

Unspecified reactive species

Lingwu Meng,Shaofei Yuan,Linjia Zhu,Zongxiao ShangGuan,Renguo Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NMBR antibody [EPR6880]

Western blot - Anti-NMBR antibody [EPR6880] (AB134141)

OI-RD Scanning - Anti-NMBR antibody [EPR6880] (AB134141)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise