AB201924

Anti-NMD3 antibody

Name: Anti-NMD3 antibody (ab201924) | Abcam
Brand: Abcam Limited
SKU: AB201924

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal NMD3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NMD3 aa 450 to C-terminus.

View Alternative Names

CGI-07, NMD3, 60S ribosomal export protein NMD3, hNMD3

2 Images

Supplier Data

Immunoprecipitation - Anti-NMD3 antibody (AB201924)

Detection of NMD3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab201924 at 6 μg/mg lysate for IP and at 0.1 μg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NMD3 antibody (ab201924)

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-NMD3 antibody (AB201924)

All lanes:

Western blot - Anti-NMD3 antibody (ab201924) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 58 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NMD3 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96D46

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab201924 was affinity purified using an epitope specific to NMD3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMD3 also known as Ribosome Export Adaptor NMD3 serves as an important shuttling protein involved in the nuclear export of the 60S ribosomal subunit. It has an estimated mass of approximately 60 kDa. The expression of NMD3 is widespread but prominently seen in cells with high proliferative activity. This protein resides in the nucleoplasm and nucleolus but translocates to the cytoplasm to facilitate its function.

Biological function summary

NMD3 plays a role in ribosomal subunit synthesis by acting as an adaptor required for the export of the mature 60S subunit from the nucleus to the cytoplasm. It does not act alone; NMD3 collaborates with several other proteins forming a transient complex with certain nucleoporins and exportins. This dynamic engagement ensures the correct timing and location of ribosome assembly a vital process for protein synthesis.

Pathways

NMD3 integrates into the ribosome biogenesis pathway an essential process for cellular growth and division. It interacts with larger complexes like the nuclear pore complex and partners with various proteins such as the exportin Crm1. NMD3’s role ensures the appropriate transport and assembly of the ribosomal subunits impacting overall protein translation efficiency.

NMD3 has connections to ribosomopathies and certain cancers. Dysregulation of NMD3 function can lead to impaired ribosomal assembly contributing to defective protein synthesis. The disruption in its function often links to overexpression or mutations in associated proteins like the nucleoporins which can contribute to the pathogenesis observed in specific cancers. Understanding the modifications in NMD3 and its associated protein interactions can provide insights into these disease processes paving the way for therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.

See full target information NMD3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 13:e0209856 PubMed30589899

2018

BioID identifies proteins involved in the cell biology of caveolae.

Applications

Unspecified application

Species

Unspecified reactive species

C Mendoza-Topaz,I Yeow,K Riento,B J Nichols

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NMD3 antibody

Immunoprecipitation - Anti-NMD3 antibody (AB201924)

Western blot - Anti-NMD3 antibody (AB201924)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise