Anti-NMDAR1 antibody [EPR2480Y] - Neuronal Marker

• WB

Lab

Western blot - Anti-NMDAR1 antibody [EPR2480Y] - Neuronal Marker (AB68144)

Blocking/diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NMDAR1 antibody [EPR2480Y] - Neuronal Marker (ab68144) at 1/1000 dilution

Lane 1:

Human fetal brain cell lysate at 15 µg

Lane 2:

Human forebrain cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 120 kDa

false

Exposure time: 30s

• WB

Lab

Western blot - Anti-NMDAR1 antibody [EPR2480Y] - Neuronal Marker (AB68144)

Anti-GRIN1 antibody [EPR2480Y] (ab68144) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab68144 was shown to bind specifically to GRIN1. First, unboiled samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-NMDAR1 antibody [EPR2480Y] - Neuronal Marker (ab68144) at 1/1000 dilution

Lane 1:

Mouse Brain (unboiled) cell lysate at 20 µg

Lane 2:

Mouse Liver (unboiled) cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 120 kDa

false