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Rabbit Recombinant Monoclonal NMDAR1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 68 publications.


Images

Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (AB109182), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (AB109182), expandable thumbnail
  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (AB109182), expandable thumbnail
  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (AB109182), expandable thumbnail
  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (AB109182), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Expected
Mouse
Tested
Not recommended
Tested
Rat
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (PubMed:26875626). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:21376300, PubMed:26875626, PubMed:26919761, PubMed:27164704, PubMed:28095420, PubMed:28105280, PubMed:28126851, PubMed:28228639, PubMed:36959261, PubMed:38538865, PubMed:7679115, PubMed:7681588, PubMed:7685113). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:26919761, PubMed:36309015, PubMed:38598639).

Alternative names

NMDAR1, GRIN1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, hNR1

Recommended products

Rabbit Recombinant Monoclonal NMDAR1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 68 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2481(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.

Biological function summary

NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.

Pathways

NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.

Associated diseases and disorders

NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 105 kDa

    Observed band size: 120 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling NMDAR1 with purified ab109182 at 1/50 (9.5μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution

    All lanes: Human cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 105 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/5000 dilution

    All lanes: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 105 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    All lanes: Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution

    All lanes: Human fetal brain cell lysate at 10 µg

    Predicted band size: 105 kDa

    Observed band size: 120 kDa

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    Western blot: Anti-GRIN1 antibody [EPR2481(2)] (ab109182) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab109182 was shown to bind specifically to GRIN1. A band was observed at 120 kDa in wild-type Neuro-2a cell lysates with no signal observed at this size in GRIN1 knockout cell line ab281960 (knockout cell lysate ab282987). To generate this image, wild-type and GRIN1 knockout Neuro-2a cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182) at 1/1000 dilution

    Lane 1: Wild-type Neuro-2a cell lysate at 20 µg

    Lane 2: GRIN1 knockout Neuro-2a cell lysate at 20 µg

    Lane 3: SH-SY5Y UNBOILED cell lysate at 20 µg

    Lane 4: THP-1 UNBOILED cell lysate at 20 µg

    Lane 5: Human Brain UNBOILED cell lysate at 20 µg

    Lane 6: Human Liver UNBOILED cell lysate at 20 µg

    Lane 7: Mouse Brain UNBOILED cell lysate at 20 µg

    Lane 8: Mouse Liver UNBOILED cell lysate at 20 µg

    Observed band size: 120 kDa

  • Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-NMDAR1 antibody [EPR2481(2)] (ab109182)

    NMDAR1 western blot using anti-NMDAR1 antibody [EPR2481(2)] ab109182. Publication image and figure legend from Li, J., Liu, Y., et al., 2019, Neural Plast, PubMed 30911292.


    ab109182 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109182 please see the product overview.

    Hippocampal synaptic plasticity-associated proteins in diabetic rats and the effects of aerobic exercise. Levels of NMDAR1 (a), SYN (b), and Homer 1 (c) were significantly lower in diabetic rats. After aerobic exercise intervention, these protein levels were significantly increased. ∗∗p < 0.01, DM group vs. C group; ##p < 0.01, TDM group vs. DM group.

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