Rabbit Polyclonal NMDAR1 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Human, Rat samples. Cited in 44 publications.
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- Cellular and molecular life sciences : CMLS 80:3682023STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarolina Serwach,Ewa Nurowska,Marta Klukowska,Barbara Zablocka,Joanna Gruszczynska-BiegalaPubMed 37989792
- Life science alliance 6:2023Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in knockout rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuo Pang,Zhuohui Luo,Wei Dong,Shan Gao,Wei Chen,Ning Liu,Xu Zhang,Xiang Gao,Jing Li,Kai Gao,Xudong Shi,Feifei Guan,Li Zhang,Lianfeng ZhangPubMed 36625203
- Frontiers in cellular neuroscience 16:10104782022The effects of preventative cannabidiol in a male mouse model of schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Visini,Samara Brown,Katrina Weston-Green,Cynthia Shannon Weickert,Rose Chesworth,Tim KarlPubMed 36406747
- Frontiers in cellular neuroscience 16:10206442022Electroacupuncture of the trigeminal nerve causes -methyl-D-aspartate receptors to mediate blood-brain barrier opening and induces neuronal excitatory changes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Gong,Shanshan Zhang,Li Ren,Jiangsong Zhang,Yibin Zhao,Xuqing Mao,Lin Gan,Hao Wang,Congcong Ma,Yubo Lin,Qinyu Ye,Kecheng Qian,Xianming LinPubMed 36313622
- Purinergic signalling 19:43-532022Spinal cord astrocyte P2X7Rs mediate the inhibitory effect of electroacupuncture on visceral hypersensitivity of rat with irritable bowel syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Jun Weng,Shi-Xiu Hu,Fang Zhang,Zhi-Ying Zhang,Yun Zhou,Min Zhao,Yan Huang,Yu-Hu Xin,Huan-Gan Wu,Hui-Rong LiuPubMed 35389158
- Frontiers in nutrition 9:8742542022A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality Regulating Brain Electrochemical Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuhammed Furkan Dasdelen,Sezgin Er,Berkan Kaplan,Suleyman Celik,Mustafa Caglar Beker,Cemal Orhan,Mehmet Tuzcu,Nurhan Sahin,Havakhanum Mamedova,Sarah Sylla,James Komorowski,Sara Perez Ojalvo,Kazim Sahin,Ertugrul KilicPubMed 35449538
- Science advances 7:eabg59702021Disrupting D1-NMDA or D2-NMDA receptor heteromerization prevents cocaine's rewarding effects but preserves natural reward processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndry Andrianarivelo,Estefani Saint-Jour,Paula Pousinha,Sebastian P Fernandez,Anna Petitbon,Veronique De Smedt-Peyrusse,Nicolas Heck,Vanesa Ortiz,Marie-Charlotte Allichon,Vincent Kappès,Sandrine Betuing,Roman Walle,Ying Zhu,Charlène Joséphine,Alexis-Pierre Bemelmans,Gustavo Turecki,Naguib Mechawar,Jonathan A Javitch,Jocelyne Caboche,Pierre Trifilieff,Jacques Barik,Peter VanhouttePubMed 34669474
- Annals of translational medicine 9:13202021Effects of N-Methyl-D-aspartate receptor (NMDAR) and Ca/calmodulin-dependent protein kinase IIα (CaMKIIα) on learning and memory impairment in depressed rats with different charge by modified electroconvulsive shock.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxi Zhang,Li Ren,Su Min,Feng Lv,Jian YuPubMed 34532457
- PloS one 16:e02569722021Chronic presence of blood circulating anti-NMDAR1 autoantibodies impairs cognitive function in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Yue,Sorana Caldwell,Victoria Risbrough,Susan Powell,Xianjin ZhouPubMed 34473764
- Pharmacology, biochemistry, and behavior 206:1732092021Donepezil prevents morphine tolerance by regulating N-methyl-d-aspartate receptor, protein kinase C and CaM-dependent kinase II expression in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Mei Zhu,Lin-Xin Wu,Bo Zhang,Yan-Peng Dong,Li SunPubMed 34058253
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC (PFA fixed) | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC (PFA fixed)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:1350383, PubMed:1388270, PubMed:1834949, PubMed:8428958). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:11823786, PubMed:1350383, PubMed:1388270, PubMed:15996549, PubMed:18177891, PubMed:1834949, PubMed:24876489, PubMed:27135925, PubMed:27618671, PubMed:28384476, PubMed:28468946, PubMed:8428958). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:10436042, PubMed:11160393, PubMed:11929923, PubMed:28384476, PubMed:9463421). Forms excitatory glycinergic receptor complexes with GluN3 alone which are activated by glycine binding to the GluN1 and GluN3 subunits (PubMed:11823786, PubMed:11929923, PubMed:12391275).
Alternative names
Nmdar1, Grin1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1
Recommended products
Rabbit Polyclonal NMDAR1 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Human, Rat samples. Cited in 44 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab274377.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.
- Biological function summary
NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.
- Pathways
NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.
- Associated diseases and disorders
NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)
NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown).
All lanes: Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/mL
Lane 1: HEK293 Whole Cell Lysate Transfected with SHAM at 10 µg
Lane 2: HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 130 kDa, 54 kDa
Exposure time: 30s
This image is courtesy of Sophie Pezet, Univ London Kings Coll, United KingdomImmunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)
ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5μg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.
Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)
HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa). ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2μg/ml.
All lanes: Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/mL
All lanes: HEK293 cell lysate co-transfected with NR1 and NR2A recombinant protein at 50 µg
Predicted band size: 105 kDa
Observed band size: ~110 kDa
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