AB17345

Anti-NMDAR1 antibody - Neuronal Marker

(6 Reviews)

(56 Publications)

Rabbit Polyclonal NMDAR1 antibody. Suitable for WB, IHC (PFA fixed) and reacts with Human, Rat samples. Cited in 56 publications.

View Alternative Names

Nmdar1, Grin1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1

3 Images

Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

IHC (PFA fixed)

Collaborator

Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5μg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details : rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.

This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom

Project

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown).

All lanes:

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/mL

Lane 1:

HEK293 Whole Cell Lysate Transfected with SHAM at 10 µg

Lane 2:

HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 105 kDa

Observed band size: 130 kDa,54 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa). ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2μg/ml.

All lanes:

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/mL

All lanes:

HEK293 cell lysate co-transfected with NR1 and NR2A recombinant protein at 50 µg

Predicted band size: 105 kDa

Observed band size: ~110 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC (PFA fixed)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab274377.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.

Biological function summary

NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.

Pathways

NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.

NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 1350383, PubMed : 1388270, PubMed : 1834949, PubMed : 8428958). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 11823786, PubMed : 1350383, PubMed : 1388270, PubMed : 15996549, PubMed : 18177891, PubMed : 1834949, PubMed : 24876489, PubMed : 27135925, PubMed : 27618671, PubMed : 28384476, PubMed : 28468946, PubMed : 8428958). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 10436042, PubMed : 11160393, PubMed : 11929923, PubMed : 28384476, PubMed : 9463421). Forms excitatory glycinergic receptor complexes with GluN3 alone which are activated by glycine binding to the GluN1 and GluN3 subunits (PubMed : 11823786, PubMed : 11929923, PubMed : 12391275).

See full target information Grin1

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

European journal of pain (London, England) 29:e70069 PubMed40552835

2025

Loss of Glutaminase 1 in Small Sensory Neurons Prevents Nerve Injury Induced Mechanical Allodynia: Insights From Conditional Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anabel Martínez-Padilla,Javier Márquez,Miguel Á Huerta,Carolina Roza,Elsa Cisneros

Research (Washington, D.C.) 7:0482 PubMed39301264

2024

Gut Microbiota-Metabolite-Brain Axis Reconstitution Reverses Sevoflurane-Induced Social and Synaptic Deficits in Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Zhao,Sanxing Ma,Lirong Liang,Shuhui Cao,Ze Fan,Danyi He,Xiaotong Shi,Yao Zhang,Bing Liu,Meiting Zhai,Shengxi Wu,Fang Kuang,Hui Zhang

Frontiers in neuroscience 18:1425447 PubMed39176383

2024

Molecular mechanisms underlying sex and treatment-dependent differences in an animal model of cue-exposure therapy for cocaine relapse prevention.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Peterson,Jonathan Nguyen,Naveed Ghani,Pedro Rodriguez-Echemendia,Hui Qiao,Sun Young Guwn,Heng-Ye Man,Kathleen M Kantak

Frontiers in neuroscience 18:1386801 PubMed38831757

2024

Rapidly repeated visual stimulation induces long-term potentiation of VEPs and increased content of membrane AMPA and NMDA receptors in the V1 cortex of cats.

Applications

Unspecified application

Species

Unspecified reactive species

Shunshun Chen,Hongyan Lu,Changning Cheng,Zheng Ye,Tianmiao Hua

Molecular neurobiology 61:9125-9135 PubMed38592586

2024

Limb-Clasping Response in NMDA Receptor Palmitoylation-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nami Suzuki,Akiko Oota-Ishigaki,Toshie Kaizuka,Masayuki Itoh,Maya Yamazaki,Rie Natsume,Manabu Abe,Kenji Sakimura,Masayoshi Mishina,Takashi Hayashi

Cell reports. Medicine 5:101413 PubMed38325382

2024

TwinF interface inhibitor FP802 stops loss of motor neurons and mitigates disease progression in a mouse model of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Yu Meng Wang,Andrea Hellwig,Hilmar Bading

Developmental dynamics : an official publication of the American Association of Anatomists 253:659-676 PubMed38193537

2024

Trim46 knockout impaired neuronal architecture and caused hypoactive behavior in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Guan,Shan Gao,Hanxuan Sheng,Yuanwu Ma,Wei Chen,Xiaolong Qi,Xu Zhang,Xiang Gao,Shuo Pang,Lianfeng Zhang,Li Zhang

Cellular and molecular life sciences : CMLS 80:368 PubMed37989792

2023

STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Serwach,Ewa Nurowska,Marta Klukowska,Barbara Zablocka,Joanna Gruszczynska-Biegala

International journal of molecular sciences 24: PubMed37511537

2023

Long-Term Treatment with Cannabidiol-Enriched Extract Induces Synaptic Changes in the Adolescent Rat Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey F L Aguiar,Raquel M P Campos,Alinny R Isaac,Yolanda Paes-Colli,Virgínia M Carvalho,Luzia S Sampaio,Ricardo A de Melo Reis

Journal of neuroscience research 101:1170-1187 PubMed36807930

2023

Metabotropic glutamate receptor 5-mediated inhibition of inward-rectifying K channel 4.1 contributes to orofacial ectopic mechanical allodynia following inferior alveolar nerve transection in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ke Li,Yan-Yan Zhang,Jiu Lin,Ya-Jing Liu,Yue-Ling Li,Yu-Heng Feng,Jia-Shuo Zhao,Cheng Zhou,Fei Liu,Jie-Fei Shen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NMDAR1 antibody - Neuronal Marker

Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB17345)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

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