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AB52177

Anti-NMDAR1 antibody - Neuronal Marker

4

(2 Reviews)

|

(24 Publications)

Rabbit Polyclonal NMDAR1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human GRIN1 aa 800 to C-terminus.

View Alternative Names

NMDAR1, GRIN1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, hNR1

2 Images
Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB52177)
  • WB

Unknown

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (AB52177)

All lanes:

Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab52177) at 1/500 dilution

Lane 1:

A549 (Human lung adenocarcinoma epithelial cell line) whole cell lysate at 10 µg

Lane 2:

Y79 (Human retinoblastoma cell line) whole cell lysate at 10 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 105 kDa

Observed band size: 30 kDa,75 kDa,95 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody - Neuronal Marker (AB52177)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR1 antibody - Neuronal Marker (AB52177)

ab52177 at 1/50 dilution staining NMDAR1 in human brain tissue by immunohistochemistry (paraffin embedded tissue) in the presence (right image) or absence (left image) of the immunizing peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GRIN1 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q05586

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR1 also known as GluN1 is a subunit of the NMDA receptor a type of ion channel protein found in the brain. This receptor weighs around 120 kDa and plays an important role in synaptic transmission and plasticity. NMDAR1 operates as part of a receptor complex that allows for calcium influx when activated. It shows high expression throughout the central nervous system primarily in neurons across the cortex and hippocampus. The receptor's function is important to certain calcium-dependent processes in the brain.
Biological function summary

NMDA receptors including the NMDAR1 subunit serve essential functions in synaptic plasticity and memory processes. As part of the receptor complex NMDAR1 contributes to the control of synaptic strength. It requires co-activation by glutamate and glycine or D-serine linking its function to excitatory neurotransmission. The receptor's capacity to modulate synaptic connections forms the basis of learning and long-term memory.

Pathways

NMDAR1 plays a critical role in the glutamatergic pathway influencing synaptic plasticity and memory function. It interacts with other proteins like PSD-95 within the postsynaptic density. NMDAR1 is also involved in the calcium signaling pathway due to its ability to facilitate calcium entry into neurons upon synaptic activity. This positions the receptor as an important modulator in pathways that control neuronal communication and plasticity.

NMDA receptor dysfunction including the NMDAR1 subunit links to neurodegenerative diseases such as Alzheimer's. The alterations in NMDAR1 function can lead to excitotoxicity contributing to neuronal damage. NMDAR1 also associates with schizophrenia where altered glutamatergic transmission has been observed. Here abnormalities in proteins like the synaptic scaffolding protein PSD-95 have also been noted to relate to NMDAR1 dysfunction in the pathophysiology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 21376300, PubMed : 26875626, PubMed : 26919761, PubMed : 28126851, PubMed : 28228639, PubMed : 36959261, PubMed : 7679115, PubMed : 7681588, PubMed : 7685113). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the long-term potentiation (LTP) (PubMed : 26875626). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 21376300, PubMed : 26875626, PubMed : 26919761, PubMed : 27164704, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28228639, PubMed : 36959261, PubMed : 38538865, PubMed : 7679115, PubMed : 7681588, PubMed : 7685113). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 or GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761, PubMed : 36309015, PubMed : 38598639).
See full target information GRIN1
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 147:2507-2521 PubMed38577773

2024

Tiam1-mediated maladaptive plasticity underlying morphine tolerance and hyperalgesia.

Applications

Unspecified application

Species

Unspecified reactive species

Changqun Yao,Xing Fang,Qin Ru,Wei Li,Jun Li,Zeinab Mehsein,Kimberley F Tolias,Lingyong Li

International journal of molecular sciences 25: PubMed38474266

2024

The Expression and Functionality of CBR-NMDAR Complexes Are Decreased in A Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Reyes-Resina,Jaume Lillo,Iu Raïch,Joan Biel Rebassa,Gemma Navarro

Neuron 111:2038-2050.e6 PubMed37146610

2023

Tiam1 coordinates synaptic structural and functional plasticity underpinning the pathophysiology of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyong Li,Qin Ru,Yungang Lu,Xing Fang,Guanxing Chen,Ali Bin Saifullah,Changqun Yao,Kimberley F Tolias

Cells 12: PubMed36672218

2023

The Expression of Cellular Prion Protein, PrPC, Favors pTau Propagation and Blocks NMDAR Signaling in Primary Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Rivas-Santisteban,Iu Raïch,David Aguinaga,Carlos A Saura,Rafael Franco,Gemma Navarro

Neurochemical research 48:791-803 PubMed36335177

2022

Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Fajuan Tang,Lin Chen,Hu Gao,Yupeng Lei,Linli Pan,Dongqiong Xiao,Xihong Li

Brain sciences 11: PubMed34942863

2021

Modulation of Hippocampal Astroglial Activity by Synaptamide in Rats with Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Manzhulo,Olga Manzhulo,Anna Tyrtyshnaia,Arina Ponomarenko,Sophia Konovalova,Ekaterina Ermolenko,Elena Milkina,Anna Starinets

International journal of molecular sciences 22: PubMed34884587

2021

Synaptamide Improves Cognitive Functions and Neuronal Plasticity in Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tyrtyshnaia,Anatoly Bondar,Sophia Konovalova,Igor Manzhulo

Alzheimer's research & therapy 13:184 PubMed34749800

2021

N-Methyl-D-aspartate (NMDA) and cannabinoid CB receptors form functional complexes in cells of the central nervous system: insights into the therapeutic potential of neuronal and microglial NMDA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Rivas-Santisteban,Alejandro Lillo,Jaume Lillo,Joan-Biel Rebassa,Joan S Contestí,Carlos A Saura,Rafael Franco,Gemma Navarro

Disease markers 2021:9990382 PubMed34093900

2021

Anti-N-Methyl-D-Aspartate Receptor Encephalitis Associated with Ovarian Teratoma in South China-Clinical Features, Treatment, Immunopathology, and Surgical Outcomes of 21 Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyun Jiang,Huixia Ye,Yifeng Wang,Yunhui Li,Ying Wang,Xiaomao Li

Experimental brain research 239:2015-2024 PubMed33909110

2021

Increased immunoreactivity of glutamate receptors, neuronal nuclear protein and glial fibrillary acidic protein in the hippocampus of epileptic rats with fast ripple activity.

Applications

Unspecified application

Species

Unspecified reactive species

Gustavo A Chiprés-Tinajero,Miguel A Núñez-Ochoa,Laura Medina-Ceja
View all publications
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