Rabbit Polyclonal NMDAR2A antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human, Rabbit samples. Cited in 10 publications. Immunogen corresponding to Recombinant Protein within Rat Grin2a.
Images
Publications
Filter by
- JCI insight 8:2023MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei ZhuPubMed 37192007
- International journal of molecular sciences 23:2022Delayed Impairment of Hippocampal Synaptic Plasticity after Pentylenetetrazole-Induced Seizures in Young Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatyana Y Postnikova,Alina M Trofimova,Maria V Zakharova,Olga I Nosova,Alexey R Brazhe,Dmitry E Korzhevskii,Alexey V Semyanov,Aleksey V ZaitsevPubMed 36362260
- International journal of molecular sciences 23:2022MTEP, a Selective mGluR5 Antagonist, Had a Neuroprotective Effect but Did Not Prevent the Development of Spontaneous Recurrent Seizures and Behavioral Comorbidities in the Rat Lithium-Pilocarpine Model of Epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra V Dyomina,Anna A Kovalenko,Maria V Zakharova,Tatiana Yu Postnikova,Alexandra V Griflyuk,Ilya V Smolensky,Irina V Antonova,Aleksey V ZaitsevPubMed 35008924
- Biochemical pharmacology 168:204-2132019Profile of cortical N-methyl-D-aspartate receptor subunit expression associates with inherent motor impulsivity in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrionna D Davis-Reyes et. al.PubMed 31295463
- Physiological reports 7:e139582019Chronic treatment with N-acetylcysteine decreases extinction responding and reduces cue-induced nicotine-seeking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGregory L Powell,Jonna M Leyrer-Jackson,Julianna Goenaga,Mark D Namba,Jose Piña,Sade Spencer,Neringa Stankeviciute,Danielle Schwartz,Nicholas P Allen,Armani P Del Franco,Erin A McClure,Michael Foster Olive,Cassandra D GipsonPubMed 30632301
- Cellular physiology and biochemistry : internation 51:1982-19952018Pituitary Adenylate Cyclase Activating Polypeptide Elicits Neuroprotection Against Acute Ischemic Neuronal Cell Death Associated with NMDA Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuji Kaneko et. al.PubMed 30513524
- PloS one 12:e01882732017Sepsis-induced selective loss of NMDA receptors modulates hippocampal neuropathology in surviving septic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuibing Zhang et. al.PubMed 29176858
- PloS one 12:e01824712017Pre-existing weakness is critical for the occurrence of postoperative cognitive dysfunction in mice of the same age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujie Tang,Xueqin Wang,Shuibing Zhang,Shangchun Duan,Wenxiang Qing,Gong Chen,Feng Ye,Yuan Le,Wen OuyangPubMed 28787017
- GeroScience 39:385-4062017Hypertension-induced synapse loss and impairment in synaptic plasticity in the mouse hippocampus mimics the aging phenotype: implications for the pathogenesis of vascular cognitive impairment.
- Nature chemical biology 12:1075-10832016Identification and characterization of PPARα ligands in the hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvik Roy et. al.PubMed 27748752
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Protein within Rat Grin2a. The exact immunogen used to generate this antibody is proprietary information. Database link Q00959
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Predicted | Predicted |
| Mouse | Expected | Predicted | Predicted |
| Rat | Expected | Tested | Expected |
| Rabbit | Predicted | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Recommended dilution: 6 ul per 200 ug lysate. |
Species Rat | Dilution info - | Notes Recommended dilution: 6 ul per 200 ug lysate. |
Species Human | Dilution info - | Notes Recommended dilution: 6 ul per 200 ug lysate. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (Probable) (PubMed:11160393, PubMed:11929923, PubMed:1350383, PubMed:14602821, PubMed:30500536). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:16281028, PubMed:23625947, PubMed:24462099, PubMed:27618671, PubMed:27916457, PubMed:28468946, PubMed:30500536, Ref.2). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:11160393, PubMed:11929923, PubMed:14602821, PubMed:23625947, PubMed:24607230, PubMed:28384476, PubMed:28760974, PubMed:9463421, Ref.2). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).
Alternative names
GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, Grin2a
Recommended products
Rabbit Polyclonal NMDAR2A antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human, Rabbit samples. Cited in 10 publications. Immunogen corresponding to Recombinant Protein within Rat Grin2a.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Protein within Rat Grin2a. The exact immunogen used to generate this antibody is proprietary information. Database link Q00959
- Purification technique
- Affinity purification Immunogen
- Specificity
ab169873 is specific for the ~180k NR2A subunit of NMDAR2A. It has no reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used in its generation.
- Concentration
- Purification notes
ab169873 is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.
- Biological function summary
NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.
- Pathways
NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.
- Associated diseases and disorders
NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-NMDAR2A antibody (ab169873)
All lanes: Western blot - Anti-NMDAR2A antibody (ab169873) at 1/1000 dilution
All lanes: Rat hippocampal lysate at 10 µg
Predicted band size: 165 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (ab169873)
Immunohistochemical analysis of rabbit retina tissue labeling NMDAR2A with ab169873 at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com