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AB169873

Anti-NMDAR2A antibody

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(17 Publications)

Rabbit Polyclonal NMDAR2A antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human, Rabbit samples. Cited in 17 publications. Immunogen corresponding to Recombinant Protein within Rat Grin2a.

View Alternative Names

GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NMDAR2A, NR2A, Grin2a

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB169873)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB169873)

Immunohistochemical analysis of rabbit retina tissue labeling NMDAR2A with ab169873 at 1/1000 dilution.

Western blot - Anti-NMDAR2A antibody (AB169873)
  • WB

Supplier Data

Western blot - Anti-NMDAR2A antibody (AB169873)

All lanes:

Western blot - Anti-NMDAR2A antibody (ab169873) at 1/1000 dilution

All lanes:

Rat hippocampal lysate at 10 µg

Predicted band size: 165 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rabbit, Rat, Human, Mouse

Applications

WB, IP, IHC-P

applications

Immunogen

Recombinant Protein within Rat Grin2a. The exact immunogen used to generate this antibody is proprietary information.

Q00959

Specificity

ab169873 is specific for the ~180k NR2A subunit of NMDAR2A. It has no reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used in its generation.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Recommended dilution: 6 ul per 200 ug lysate.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Recommended dilution: 6 ul per 200 ug lysate.</p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Recommended dilution: 6 ul per 200 ug lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab169873 is prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.
Biological function summary

NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.

Pathways

NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.

NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (Probable) (PubMed : 11160393, PubMed : 11929923, PubMed : 1350383, PubMed : 14602821, PubMed : 30500536). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 16281028, PubMed : 23625947, PubMed : 24462099, PubMed : 27618671, PubMed : 27916457, PubMed : 28468946, PubMed : 30500536, Ref.2). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 11160393, PubMed : 11929923, PubMed : 14602821, PubMed : 23625947, PubMed : 24607230, PubMed : 28384476, PubMed : 28760974, PubMed : 9463421, Ref.2). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).
See full target information Grin2a
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009625

2025

Fenofibrate as a PPARα Agonist Modulates Neuroinflammation and Glutamate Receptors in a Rat Model of Temporal Lobe Epilepsy: Region-Specific Effects and Behavioral Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Anna A Kovalenko,Maria V Zakharova,Olga E Zubareva,Alexander P Schwarz,Yury A Skorik,Aleksey V Zaitsev

Brain communications 7:fcaf124 PubMed40226380

2025

Absence of GluN2A in hippocampal CA1 neurons leads to altered dendritic structure and reduced frequency of miniature excitatory synaptic events.

Applications

Unspecified application

Species

Unspecified reactive species

Farhana Yasmin,Katie F M Marwick,Daniel W Hunter,Sarfaraz Nawaz,Grant F Marshall,Sam A Booker,Giles E Hardingham,Peter C Kind,David J A Wyllie

Molecular autism 15:28 PubMed38877552

2024

Enhanced hippocampal LTP but normal NMDA receptor and AMPA receptor function in a rat model of CDKL5 deficiency disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Simões de Oliveira,Heather E O'Leary,Sarfaraz Nawaz,Rita Loureiro,Elizabeth C Davenport,Paul Baxter,Susana R Louros,Owen Dando,Emma Perkins,Julien Peltier,Matthias Trost,Emily K Osterweil,Giles E Hardingham,Michael A Cousin,Sumantra Chattarji,Sam A Booker,Tim A Benke,David J A Wyllie,Peter C Kind

JCI insight 8: PubMed37192007

2023

MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei Zhu

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:4513-4524 PubMed37160364

2023

Hypothalamic Corticotropin-Releasing Hormone Contributes to Hypertension in Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Zhang,Jing-Jing Zhou,Jian-Ying Shao,Zhao-Fu Sheng,Jingxiong Wang,Peiru Zheng,Xunlei Kang,Zhenguo Liu,Zixi Jack Cheng,David D Kline,De-Pei Li

International journal of molecular sciences 23: PubMed36362260

2022

Delayed Impairment of Hippocampal Synaptic Plasticity after Pentylenetetrazole-Induced Seizures in Young Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana Y Postnikova,Alina M Trofimova,Maria V Zakharova,Olga I Nosova,Alexey R Brazhe,Dmitry E Korzhevskii,Alexey V Semyanov,Aleksey V Zaitsev

International journal of molecular sciences 23: PubMed35008924

2022

MTEP, a Selective mGluR5 Antagonist, Had a Neuroprotective Effect but Did Not Prevent the Development of Spontaneous Recurrent Seizures and Behavioral Comorbidities in the Rat Lithium-Pilocarpine Model of Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra V Dyomina,Anna A Kovalenko,Maria V Zakharova,Tatiana Yu Postnikova,Alexandra V Griflyuk,Ilya V Smolensky,Irina V Antonova,Aleksey V Zaitsev

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 17:470-486 PubMed34741242

2021

Different Roles of Beclin1 in the Interaction Between Glia and Neurons after Exposure to Morphine and the HIV- Trans-Activator of Transcription (Tat) Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Lapierre,Mohan K M Karuppan,Marissa Perry,Myosotys Rodriguez,Nazira El-Hage

Frontiers in aging neuroscience 13:671206 PubMed34113246

2021

Cornel Iridoid Glycoside Protects Against STAT1-Dependent Synapse and Memory Deficits by Increasing -Methyl-D-aspartate Receptor Expression in a Tau Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Denglei Ma,Rui Huang,Kaiwen Guo,Zirun Zhao,Weipeng Wei,Lihong Gu,Lin Li,Lan Zhang

Cytotherapy 22:106-113 PubMed31983606

2020

Rhynchophylline promotes stem cell autonomous metabolic homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuji Kaneko,Alexandreya B Coats,Julian P Tuazon,Michiko Jo,Cesar V Borlongan
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