AB203197

Anti-NMDAR2A antibody

Name: Anti-NMDAR2A antibody (ab203197) | Abcam
Brand: Abcam Limited
SKU: AB203197
Price: 535 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(7 Publications)

Rabbit Polyclonal NMDAR2A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human GRIN2A aa 850-900 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NMDAR2A, GRIN2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NR2A, hNR2A

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody (AB203197)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody (AB203197)

Immunofluorescence analysis of U251 cells labeling NMDAR2A with ab203197. Cells were fixed with Paraformaldehyde, permeabilized with 0.3% Triton-100 for 20minutes and blocked with normal goat serum at 37^oC for 30 minutes. ab203197 at 1 : 200 dilution was incubated overnight at 4^oC. Unconjugated Goat Anti - rabbit IgG was used as a secondary antibody for 90 minutes. DAPI used as counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB203197)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB203197)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling NMDAR2A with ab203197 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB203197)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat skin tissue labeling NMDAR2A with ab203197 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB203197)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue labeling NMDAR2A with ab203197 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody (AB203197)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat skin tissue labeling NMDAR2A with ab203197 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GRIN2A aa 850-900 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q12879

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Use at 1/50 - 1/200 with fluorescent detection methods. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.

Biological function summary

NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.

Pathways

NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.

NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 20890276, PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 28242877, PubMed : 36117210, PubMed : 38538865, PubMed : 8768735). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 27288002, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28182669, PubMed : 29644724, PubMed : 38307912, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).

See full target information GRIN2A

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:698-707 PubMed39633045

2024

Macrophages excite muscle spindles with glutamate to bolster locomotion.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyang Yan,Nuria Antolin,Luming Zhou,Luyang Xu,Irene Lisa Vargas,Carlos Daniel Gomez,Guiping Kong,Ilaria Palmisano,Yi Yang,Jessica Chadwick,Franziska Müller,Anthony M J Bull,Cristina Lo Celso,Guido Primiano,Serenella Servidei,Jean François Perrier,Carmelo Bellardita,Simone Di Giovanni

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22334 PubMed35486004

2022

IL-20 promotes cutaneous inflammation and peripheral itch sensation in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiping Lu,Song Xiao,Weiwei Chen,Renkai Zhu,Hua Yang,Martin Steinhoff,Yanqing Li,Wenke Cheng,Xinrong Yan,Lianlian Li,Shanghai Xue,Ciara Larkin,Wenhao Zhang,Qianqian Fan,Ruizhen Wang,Jiafu Wang,Jianghui Meng

Frontiers in nutrition 9:874254 PubMed35449538

2022

A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality Regulating Brain Electrochemical Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammed Furkan Dasdelen,Sezgin Er,Berkan Kaplan,Suleyman Celik,Mustafa Caglar Beker,Cemal Orhan,Mehmet Tuzcu,Nurhan Sahin,Havakhanum Mamedova,Sarah Sylla,James Komorowski,Sara Perez Ojalvo,Kazim Sahin,Ertugrul Kilic

Brain sciences 11: PubMed34942863

2021

Modulation of Hippocampal Astroglial Activity by Synaptamide in Rats with Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Manzhulo,Olga Manzhulo,Anna Tyrtyshnaia,Arina Ponomarenko,Sophia Konovalova,Ekaterina Ermolenko,Elena Milkina,Anna Starinets

Frontiers in neuroscience 15:703044 PubMed34658758

2021

GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jing Han,Jing Zhu,Mizhen Zhang,Minda Ju,Yueshan Du,Zhanzhuang Tian

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine 40:163-173 PubMed32681671

2020

A Safety Study of the Effects of 2-Dimensional Shear Wave Elastography on Synaptic Morphologic Characteristics and Function in the Hippocampus of Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhang,Nan Li,Changtian Li,Junlai Li

Journal of biological rhythms 34:39-50 PubMed30654688

2019

Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat.

Applications

Unspecified application

Species

Unspecified reactive species

J Manuel Herrera-Zamora,Luis A Castro-Sánchez,Miriam Reyes-Mendez,Irving Aguilar-Martinez,Fernando Osuna-López,Eloy G Moreno-Galindo,Ricardo A Navarro-Polanco,Raul A Aguilar-Roblero,Enrique Sánchez-Pastor,Javier Alamilla

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