Anti-NMDAR2A antibody [EPR2465(2)]

• WB

Lab

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

Exposure time

Lanes 1 to 2 : 40 seconds
Lanes 3 to 6 : 20 seconds

We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

We are unsure how to define the extra bands.

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/2000 dilution

Lane 1:

Human brain lysates prepared in RIPA lysis method at 20 µg

Lane 2:

Human brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Lane 3:

Mouse brain lysates prepared in RIPA lysis method at 20 µg

Lane 4:

Mouse brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Lane 5:

Rat brain lysates prepared in RIPA lysis method at 20 µg

Lane 6:

Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 165 kDa

Observed band size: 165 kDa

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• WB

Unknown

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/20000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 165 kDa

Observed band size: 165 kDa

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• WB

Unknown

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution

All lanes:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 165 kDa

Observed band size: 165 kDa

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• WB

Unknown

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (ab124913) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Human brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 165 kDa

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• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-NMDAR2A antibody [EPR2465(2)] (AB124913)

NMDAR2A western blot using anti-NMDAR2A antibody [EPR2465(2)] ab124913. Publication image and figure legend from Liang, L., Xie, R., et al., 2020, J Neurochem, PubMed 31705656.

ab124913 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124913 please see the product overview.

Effects of antagonizing homodomain interacting protein kinase 2 on the synaptic toxicity of Sev. (a, b) Golgi staining and quantification of spines in control rats, Sev-treated, A64-treated, and Sev plus A64-treated rats. N = 6 rats per group. (c) Synaptic ultrastructure in hippocampus of control rats, A64-treated, Sev-treated, and Sev plus A64-treated rats. Notice that the decrease in spine number and PSD length were partially rescued by A64. N = 6 rats per group. (d, e) Western blot and quantification of Synaptophysin, post-synaptic density protein 95 (PSD95), calcium/calmodulin-dependent kinase II (CaMKII), phosphorylation of N-methyl-d-aspartate receptor subunit 2 A (pNR2A), NR2A and vesicular inhibitory amino acid transporter (vGAT). Notice that the decrease in Synaptophysin, PSD95, CaMKII, and pNR2A were partially rescued by A64. N = 6 rats per group. (f–h) Open field assay. N = 8 rats per group. (i–k) Elevated plus maze assay. Notice that A64 treatment alleviated the Sev-induced anxiety. N = 8 rats per group. (l–n) Morris water maze test. Notice that A64 treatment partially improved the spatial memory of Sev-treated rats. N = 8 rats per group. Con, control. Sev, Sevoflurane. *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. #p < 0.05. One-way anova (a–n). Bar = 10 μm (a) and 200 nm (c).

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