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AB133265

Anti-NMDAR2A antibody [EPR7063]

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(23 Publications)

Rabbit Recombinant Monoclonal NMDAR2A antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 23 publications.

View Alternative Names

NMDAR2A, GRIN2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NR2A, hNR2A

2 Images
Western blot - Anti-NMDAR2A antibody [EPR7063] (AB133265)
  • WB

Unknown

Western blot - Anti-NMDAR2A antibody [EPR7063] (AB133265)

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution

All lanes:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 165 kDa

false

Western blot - Anti-NMDAR2A antibody [EPR7063] (AB133265)
  • WB

Unknown

Western blot - Anti-NMDAR2A antibody [EPR7063] (AB133265)

All lanes:

Western blot - Anti-NMDAR2A antibody [EPR7063] (ab133265) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg/mL

Lane 2:

Rat brain lysate at 10 µg/mL

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 165 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7063

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.
Biological function summary

NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.

Pathways

NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.

NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 20890276, PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 28242877, PubMed : 36117210, PubMed : 38538865, PubMed : 8768735). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 27288002, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28182669, PubMed : 29644724, PubMed : 38307912, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).
See full target information GRIN2A
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurodevelopmental disorders 17:36 PubMed40604385

2025

Novel ANKRD17 variants implicate synaptic and mitochondrial disruptions in intellectual disability and autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xia,Yuanyuan Xu,Zhanwen He,Rui Chen,Xiaoqin Xiao,Xiaojuan Li,Kewen Deng,Shuyun Deng,Lina Zhang,Jieming Zhang,Xiaofang Peng,Zhe Meng,Ruohao Wu,Dilong Wang,Zulin Liu,Hui Chen,Lu Li,Liyang Liang

Science advances 10:eadk3198 PubMed38394205

2024

Long-lasting forms of plasticity through patterned ultrasound-induced brainwave entrainment.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Jeong Kim,Tien Thuy Phan,Keunhyung Lee,Jeong Sook Kim,Sang-Yeong Lee,Jung Moo Lee,Jongrok Do,Doyun Lee,Sung-Phil Kim,Kyu Pil Lee,Jinhyoung Park,C Justin Lee,Joo Min Park

Physiological reports 11:e15673 PubMed37078449

2023

Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Yokokawa,Kohei Kido,Koji Sato,Tatsuya Hayashi,Satoshi Fujita

iScience 26:106701 PubMed37207277

2023

Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper Lage

Cell reports 41:111771 PubMed36476879

2022

NMDA receptor hypofunction underlies deficits in parvalbumin interneurons and social behavior in neuroligin 3 R451C knockin mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Cao,Jia-Hui Li,Shen Lin,Qiang-Qiang Xia,Yong-Lan Du,Qian Yang,Ying-Zhi Ye,Ling-Hui Zeng,Xiang-Yao Li,Junyu Xu,Jian-Hong Luo

Frontiers in aging neuroscience 14:934230 PubMed35847668

2022

Sevoflurane Exposure in the Developing Brain Induces Hyperactivity, Anxiety-Free, and Enhancement of Memory Consolidation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Bei Wang,Xiaohong Cao,Chao Li,Yuhan Hu,Dandan Yan,Yanchang Yang,Liqing Wang,Lingzhong Meng,Zhiyong Hu

Frontiers in aging neuroscience 13:772980 PubMed34916926

2021

Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xu,Yiying Zhou,Linbo Hu,Hongde Jiang,Yibei Dong,Haowei Shen,Zhongze Lou,Siyu Yang,Yunxin Ji,Liemin Ruan,Xiaoqin Zhang

Molecular pain 17:1744806921990934 PubMed33590786

2021

Pra-C exerts analgesic effect through inhibiting microglial activation in anterior cingulate cortex in complete Freund's adjuvant-induced mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Jie Su,Long-Fei Li,Sai-Ying Wang,Qi Yang,Yu-Jing Wu,Ming-Gao Zhao,Le Yang

Neuron 109:1013-1028.e9 PubMed33548174

2021

Gating of hippocampal rhythms and memory by synaptic plasticity in inhibitory interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Xingzhi He,Jiarui Li,Guangjun Zhou,Jing Yang,Sam McKenzie,Yanjun Li,Wenwen Li,Jun Yu,Yang Wang,Jing Qu,Zhiying Wu,Hailan Hu,Shumin Duan,Huan Ma

Communications biology 3:665 PubMed33184446

2020

Phosphorylation of NMDA receptors by cyclin B/CDK1 modulates calcium dynamics and mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Jacaranda Rosendo-Pineda,Juan Jesus Vicente,Oscar Vivas,Jonathan Pacheco,Arlet Loza-Huerta,Alicia Sampieri,Linda Wordeman,Claudia Moreno,Luis Vaca
View all publications
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