AB227233

Anti-NMDAR2A antibody - N-terminal

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(4 Publications)

Rabbit Polyclonal NMDAR2A antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRIN2A.

View Alternative Names

NMDAR2A, GRIN2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NR2A, hNR2A

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody - N-terminal (AB227233)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody - N-terminal (AB227233)

Paraffin-embedded U87 (human) xenograft tissue stained for NMDAR2A using ab227233 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody - N-terminal (AB227233)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody - N-terminal (AB227233)

4% paraformaldehyde-fixed DIV14 rat E18 primary cortical neurons stained for NMDAR2A (green) using ab227233 at 1/500 dilution in ICC/IF.

Red : beta Tubulin 3 stained by a beta Tubulin 3 antibody at 1/500 dilution.
Blue : DAPI.

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

WB

Supplier Data

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

5% SDS PAGE gel.

All lanes:

Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 165 kDa

false

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

WB

Supplier Data

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution

All lanes:

Rat brain extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 165 kDa

true

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

WB

Supplier Data

Western blot - Anti-NMDAR2A antibody - N-terminal (AB227233)

5% SDS PAGE gel.

All lanes:

Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 165 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

Immunogen

Recombinant Fragment Protein within Human GRIN2A. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.

Biological function summary

NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.

Pathways

NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.

NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 20890276, PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 28242877, PubMed : 36117210, PubMed : 38538865, PubMed : 8768735). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 27288002, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28182669, PubMed : 29644724, PubMed : 38307912, PubMed : 8768735). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).

See full target information GRIN2A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed41007649

2025

Inhibiting Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tao,Lihua Yuan,Yuntian Ding,Rongli Xie,Fangjie Liu,Zhongming Zhang,Xiaojun Xu,Xiaobo Wang

Brain : a journal of neurology 147:2128-2143 PubMed38226680

2024

Maf1 loss regulates spinogenesis and attenuates cognitive impairment in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Han,Kui Chen,Hongxiang Yu,Can Cui,Hongxia Li,Yongbo Hu,Bei Zhang,Gang Li

Brain and behavior 13:e2867 PubMed36585899

2023

The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia-reperfusion injury via regulating NR2B-ERK/CREB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xialing Tang,Shanshan Xie,Huajun Wang,Yingbin Li,Zhiyu Lai,Shuangxi Sun,Ruanhuan Pan,Yan Huang,Jun Cai

Frontiers in neuroscience 15:525615 PubMed33692666

2021

Icaritin Alleviates Glutamate-Induced Neuronal Damage by Inactivating GluN2B-Containing NMDARs Through the ERK/DAPK1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Song Liu,Chaoming Liu,Lijiao Xiong,Jiali Xie,Cheng Huang,Rongbiao Pi,Zhihua Huang,Liangdong Li

View all publications

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