Rabbit Polyclonal NMDAR2A antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRIN2A.
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- Brain and behavior 13:e28672023The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia-reperfusion injury via regulating NR2B-ERK/CREB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXialing Tang,Shanshan Xie,Huajun Wang,Yingbin Li,Zhiyu Lai,Shuangxi Sun,Ruanhuan Pan,Yan Huang,Jun CaiPubMed 36585899
- Frontiers in neuroscience 15:5256152021Icaritin Alleviates Glutamate-Induced Neuronal Damage by Inactivating GluN2B-Containing NMDARs Through the ERK/DAPK1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Liu,Chaoming Liu,Lijiao Xiong,Jiali Xie,Cheng Huang,Rongbiao Pi,Zhihua Huang,Liangdong LiPubMed 33692666
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GRIN2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q12879
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:20890276, PubMed:23933818, PubMed:23933819, PubMed:23933820, PubMed:24504326, PubMed:26875626, PubMed:26919761, PubMed:28242877, PubMed:36117210, PubMed:38538865, PubMed:8768735). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:23933818, PubMed:23933819, PubMed:23933820, PubMed:24504326, PubMed:26875626, PubMed:26919761, PubMed:27288002, PubMed:28095420, PubMed:28105280, PubMed:28126851, PubMed:28182669, PubMed:29644724, PubMed:38307912, PubMed:8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:26919761). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).
Alternative names
NMDAR2A, GRIN2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NR2A, hNR2A
Recommended products
Rabbit Polyclonal NMDAR2A antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRIN2A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GRIN2A. The exact immunogen used to generate this antibody is proprietary information. Database link Q12879
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.
- Biological function summary
NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.
- Pathways
NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.
- Associated diseases and disorders
NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution
All lanes: Rat brain extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 165 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody - N-terminal (ab227233)
4% paraformaldehyde-fixed DIV14 rat E18 primary cortical neurons stained for NMDAR2A (green) using ab227233 at 1/500 dilution in ICC/IF.
Red: beta Tubulin 3 stained by a beta Tubulin 3 antibody at 1/500 dilution.
Blue: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody - N-terminal (ab227233)
Paraffin-embedded U87 (human) xenograft tissue stained for NMDAR2A using ab227233 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)
5% SDS PAGE gel.
All lanes: Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution
All lanes: Mouse brain lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 165 kDa
Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)
5% SDS PAGE gel.
All lanes: Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution
All lanes: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 165 kDa
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