AB174636

Anti-NMDAR2A antibody [N327A/38] - N-terminal

Name: Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) | Abcam
Brand: Abcam Limited
SKU: AB174636
Rating: 1 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal NMDAR2A antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Transfected cell lysate, Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Grin2a aa 50-350.

View Alternative Names

NMDAR2A, GRIN2A, GluN2A, Glutamate [NMDA] receptor subunit epsilon-1, N-methyl D-aspartate receptor subtype 2A, NR2A, hNR2A

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (AB174636)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (AB174636)

4% formaldehyde-fixed SK-N-BE cells labeling NMDAR2A using ab174636 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/200 dilution incubated for 1 hour at room temperature. Counter stained with Phalloidin Texas Red F-actin stain and DAPI (blue) nuclear stain.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [N327A/38] - N-terminal (AB174636)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling NMDAR2A with ab174636 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.

Supplier Data

Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (AB174636)

Color Development : ECL solution for 6 min in RT.

All lanes:

Western blot - Anti-NMDAR2A antibody [N327A/38] - N-terminal (ab174636) at 1/200 dilution

Lane 1:

Monkey COS cells transfected with GFP-tagged NR2A

Lane 1:

Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP for 1 hour RT. at 1/1000 dilution

Predicted band size: 165 kDa

Observed band size: ~170 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N327A/38

Isotype

IgG2b

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-P, ICC/IF, IHC-Fr, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Grin2a aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q00959

Epitope

extracellular domain

Specificity

Does not react with NMDA2B

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Reactivity data

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Product details

The clone number has been updated from S327A-38 to N327A/38, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2A also known as GRIN2A is a subunit of the N-methyl-D-aspartate (NMDA) receptor which belongs to the family of ionotropic glutamate receptors. This receptor functions as a ligand-gated ion channel and requires both glutamate and glycine for activation. NMDAR2A has a molecular mass of about 164 kDa. It plays a significant role in synaptic transmission and plasticity and is mainly expressed in the brain particularly in the cerebral cortex and hippocampus. Its expression is important for normal brain function affecting learning and memory.

Biological function summary

NMDAR2A participates in synaptic signaling by forming part of the NMDA receptor complex. As part of this complex NMDAR2A interacts with other receptor subunits such as NR1 and NR2B which together modulate calcium influx into the neuron. This influx is essential for activating downstream signaling pathways that influence synaptic strength. The receptor's activation is tightly regulated which helps to maintain the balance between synaptic excitation and inhibition in the central nervous system.

Pathways

NMDAR2A serves essential roles in the long-term potentiation (LTP) and synaptic plasticity pathways. LTP is a process that strengthens synaptic connections and is important for memory formation. NMDAR2A interacts with proteins like postsynaptic density protein 95 (PSD-95) within these pathways to mediate downstream signaling events. It is also involved in the MAPK/ERK pathway affecting cellular processes such as gene expression differentiation and survival.

NMDAR2A has been associated with epilepsy and schizophrenia. Mutations or altered expression of NMDAR2A can disrupt normal receptor function and contribute to these conditions. It shows links with other proteins such as NR2B where imbalances between different NMDA receptor subunits may exacerbate disease pathology. Understanding how NMDAR2A functions and misfunctions helps in investigating therapeutic targets for these neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 20890276, PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 28242877, PubMed : 36117210, PubMed : 38538865, PubMed : 8768735). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 23933818, PubMed : 23933819, PubMed : 23933820, PubMed : 24504326, PubMed : 26875626, PubMed : 26919761, PubMed : 27288002, PubMed : 28095420, PubMed : 28105280, PubMed : 28126851, PubMed : 28182669, PubMed : 29644724, PubMed : 38307912, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 26919761). Participates in the synaptic plasticity regulation through activation by the L-glutamate releaseed by BEST1, into the synaptic cleft, upon F2R/PAR-1 activation in astrocyte (By similarity).

See full target information GRIN2A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113489 PubMed41019369

2025

Therapeutic potential of 5HT2CR in alleviating spasticity and pain via modulation of motor neurons and interneurons in spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Han Gong,Zuliyaer Talifu,Yu-Zhe Sun,Zhao-Ming Yue,Xiao-Xia Du,Liang-Jie Du

Annals of clinical and translational neurology 11:2891-2903 PubMed39291779

2024

α-Synuclein species in plasma neuron-derived extracellular vesicles as biomarkers for iRBD.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Wang,Yuanchu Zheng,Huihui Cai,Wenyi Kou,Chen Yang,Siming Li,Bingxu Zhu,Jiayi Wu,Ning Zhang,Tao Feng,Xiaohong Li,Fulong Xiao,Zhenwei Yu

Progress in neurobiology 216:102288 PubMed35654209

2022

Brain Dp140 alters glutamatergic transmission and social behaviour in the mdx52 mouse model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yasumasa Hashimoto,Hiroshi Kuniishi,Kazuhisa Sakai,Yuta Fukushima,Xuan Du,Kunihiko Yamashiro,Kei Hori,Michihiro Imamura,Mikio Hoshino,Mitsuhiko Yamada,Toshiyuki Araki,Hiroyuki Sakagami,Shin'ichi Takeda,Keiji Itaka,Noritaka Ichinohe,Francesco Muntoni,Masayuki Sekiguchi,Yoshitsugu Aoki

International journal of molecular sciences 21: PubMed33203061

2020

Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Woojin Na,Eun-Jung Lee,Min-Kyung Kang,Yun-Ho Kim,Dong Yeon Kim,Hyeongjoo Oh,Soo-Il Kim,Su Yeon Oh,Young-Hee Kang

Journal of integrative neuroscience 18:387-392 PubMed31912696

2020

The glutamatergic system and astrocytic impairment in rat hippocampus: a comparative study of underlying etiology and pathophysiology of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Jiang,Quan-Ai Zhang,Qin Guo,Zhong Di

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