AB183942

Anti-NMDAR2B antibody [EPR19295]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(16 Publications )

Rabbit Recombinant Monoclonal NMDAR2B antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 16 publications.

2 Images

Supplier Data

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,3,4,5,6 and 7 : 30 seconds; Lane 8 and 9 : 8 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 9252405; PMID : 15470155).

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/1000 dilution

Lane 1:

Human hippocampus lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

Mouse liver lysate at 20 µg

Lane 5:

Mouse kidney lysate at 20 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 7:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 8:

Mouse brain lysate at 20 µg

Lane 9:

Mouse hippocampus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Supplier Data

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 9252405; PMID : 15470155).

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR19295] (ab183942) at 1/5000 dilution

Lane 1:

Rat brain lysate at 20 µg

Lane 2:

Rat hippocampus lysate at 20 µg

Lane 3:

Rat liver lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Exposure time: 8s

Carrier free

Anti-NMDAR2B antibody [EPR19295] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19295

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.

Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

See full target information GRIN2B

Alternative Names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

style

left-column

Publications (16)

Recent publications for all applications. Explore the full listand refine your search

Addiction neuroscience 15: PubMed40575352

2025

EcoHIV infection effects on cocaine seeking and neuroimmune responses in male mice depend on cocaine exposure pattern.

Applications

Unspecified application

Species

Unspecified reactive species

Mark D Namba,Qiaowei Xie,Kyewon Park,Joshua G Jackson,Jacqueline M Barker

Research (Washington, D.C.) 7:0482 PubMed39301264

2024

Gut Microbiota-Metabolite-Brain Axis Reconstitution Reverses Sevoflurane-Induced Social and Synaptic Deficits in Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Youyi Zhao,Sanxing Ma,Lirong Liang,Shuhui Cao,Ze Fan,Danyi He,Xiaotong Shi,Yao Zhang,Bing Liu,Meiting Zhai,Shengxi Wu,Fang Kuang,Hui Zhang

CNS neuroscience & therapeutics 30:e14632 PubMed38366763

2024

Pathological features and molecular signatures of early olfactory dysfunction in 3xTg-AD model mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Yu,Fangzhou Wang,Dongdong Jia,Shuguang Bi,Juan Gong,Jia-Jun Wu,Yumin Mao,Jia Chen,Gao-Shang Chai

Physiological reports 11:e15673 PubMed37078449

2023

Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Yokokawa,Kohei Kido,Koji Sato,Tatsuya Hayashi,Satoshi Fujita

iScience 25:104754 PubMed35938049

2022

The endoplasmic reticulum membrane complex promotes proteostasis of GABA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Angela L Whittsette,Ya-Juan Wang,Ting-Wei Mu

The European journal of neuroscience 55:18-31 PubMed34902209

2021

Hippocampal AMPA- and NMDA-induced cGMP signals are mainly generated by NO-GC2 and are under tight control by PDEs 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Giesen,Evanthia Mergia,Doris Koesling,Michael Russwurm

Frontiers in aging neuroscience 13:772980 PubMed34916926

2021

Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xu,Yiying Zhou,Linbo Hu,Hongde Jiang,Yibei Dong,Haowei Shen,Zhongze Lou,Siyu Yang,Yunxin Ji,Liemin Ruan,Xiaoqin Zhang

International journal of molecular sciences 22: PubMed34830164

2021

Nitric Oxide-Dependent Mechanisms Underlying MK-801- or Scopolamine-Induced Memory Dysfunction in Animals: Mechanistic Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Cieślik,Anna Siekierzycka,Adrianna Radulska,Agata Płoska,Grzegorz Burnat,Piotr Brański,Leszek Kalinowski,Joanna M Wierońska

Frontiers in neuroscience 15:641284 PubMed33994921

2021

Valproic Acid Induces Autism-Like Synaptic and Behavioral Deficits by Disrupting Histone Acetylation of Prefrontal Cortex ALDH1A1 in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Mei Tan,Boli Cheng,Si Wang,Lu Xiao,Jiang Zhu,Qionghui Wu,Xi Lai,Qian Zhang,Jie Chen,Tingyu Li

Oxidative medicine and cellular longevity 2021:6634181 PubMed33833851

2021

Low-Dose Copper Exposure Exacerbates Depression-Like Behavior in ApoE4 Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Xu,Kaiwu He,Kaiqin Zhang,Chao Yang,Lulin Nie,Ding Dan,Jianjun Liu,Chang-E Zhang,Xifei Yang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NMDAR2B antibody [EPR19295]

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Western blot - Anti-NMDAR2B antibody [EPR19295] (AB183942)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (16)

Product promise