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AB254356

Anti-NMDAR2B antibody [EPR23460-119]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(14 Publications)

Rabbit Recombinant Monoclonal NMDAR2B antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 14 publications.

View Alternative Names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human cerebrum (PMID : 7931566). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the rat hippocampus (PMID : 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse hippocampus (PMID : 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • IP

Unknown

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

NMDAR2B was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 μg with ab254356 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 μg.

Lane 2 : ab254356 IP in mouse brain tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254356 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

All lanes:

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (ab254356)

Predicted band size: 166 kDa

false

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • IP

Unknown

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

NMDAR2B was immunoprecipitated from 0.35 mg rat brain tissue lysate 10 μg with ab254356 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 μg.

Lane 2 : ab254356 IP in rat brain tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254356 in rat brain tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

All lanes:

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (ab254356)

Predicted band size: 166 kDa

false

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • WB

Lab

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Bands of 180KDa, 150KDa and 115KDa respectively represent the full length and endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (ab254356) at 1/1000 dilution

All lanes:

Human hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 166 kDa

false

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)
  • WB

Lab

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (AB254356)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Bands of 180KDa and 115KDa respectively represent the full length and endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID : 22523092)

Negative control : Liver (PMID : 10049997, 9712644).

Exposure time : 10 seconds.

Lanes 1, 2, 4 and 5:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (ab254356) at 1/2000 dilution

Lane 3:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (ab254356) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 10 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

Rat hippocampus tissue lysate at 10 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

false

  • Carrier free

    Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23460-119

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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  • 5
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.
Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
See full target information GRIN2B
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 21:807-826 PubMed39635882

2024

Upregulation of ISG15 induced by MAPT/tau accumulation represses autophagic flux by inhibiting HDAC6 activity: a vicious cycle in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Xin Wang,Zhi-Ting Fang,Jun-Ning Zhao,Xue-Xiang Rui,Bing-Ge Zhang,Ye He,Rui-Juan Liu,Jian Chen,Gao-Shang Chai,Gong-Ping Liu

Brain research 1845:149284 PubMed39423961

2024

2-BFI protects against ischemic stroke by selectively acting on NR2B-containing NMDA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Xu,Jiaou Chen,Chunfei Xu,Ye Xu,Lu Xu,Meiqi Zhao,Tong Xu,Yungang Cao,Peijun Li,Zhao Han

CNS neuroscience & therapeutics 30:e14750 PubMed38898731

2024

Cognitive decline in Sprague-Dawley rats induced by neuroplasticity changes after occlusal support loss.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wang,Qian Pang,Jiangqi Hu,Bin Luo,Yunping Lu,Xu Sun,Shixiang Meng,Qingsong Jiang

ACS omega 9:21838-21850 PubMed38799363

2024

Activation of GPR55 Ameliorates Maternal Separation-Induced Learning and Memory Deficits by Augmenting 5-HT Synthesis in the Dorsal Raphe Nucleus of Juvenile Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Sun,Ya-Ya Du,Yong-Qiang Zhang,Qin-Qin Tian,Xi Li,Jiao-Yan Yu,Yan-Yan Guo,Qing-Qing Liu,Le Yang,Yu-Mei Wu,Qi Yang,Ming-Gao Zhao

Neural regeneration research 19:915-922 PubMed37843229

2023

STAT3 ameliorates truncated tau-induced cognitive deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Bingge Zhang,Huali Wan,Maimaitijian Maierwufu,Qian Liu,Ting Li,Ye He,Xin Wang,Gongping Liu,Xiaoyue Hong,Qiong Feng

Aging cell 22:e13932 PubMed37594184

2023

ApoE4 exacerbates the senescence of hippocampal neurons and spatial cognitive impairment by downregulating acetyl-CoA level.

Applications

Unspecified application

Species

Unspecified reactive species

Shuixin Lv,Yusi Zhang,Yingbin Lin,Wenting Fang,Yu Wang,Zihang Li,Anlan Lin,Xiaoman Dai,Qinyong Ye,Jing Zhang,Xiaochun Chen

BMC medicine 21:223 PubMed37365538

2023

Aberrant palmitoylation caused by a ZDHHC21 mutation contributes to pathophysiology of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Li,Yana Pang,Yan Wang,Fan Mei,Mengmeng Guo,Yiping Wei,Xinyue Li,Wei Qin,Wei Wang,Longfei Jia,Jianping Jia

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:4513-4524 PubMed37160364

2023

Hypothalamic Corticotropin-Releasing Hormone Contributes to Hypertension in Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Zhang,Jing-Jing Zhou,Jian-Ying Shao,Zhao-Fu Sheng,Jingxiong Wang,Peiru Zheng,Xunlei Kang,Zhenguo Liu,Zixi Jack Cheng,David D Kline,De-Pei Li

Brain and behavior 13:e2867 PubMed36585899

2023

The combination of Astragalus membranaceus and ligustrazine mitigates cerebral ischemia-reperfusion injury via regulating NR2B-ERK/CREB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xialing Tang,Shanshan Xie,Huajun Wang,Yingbin Li,Zhiyu Lai,Shuangxi Sun,Ruanhuan Pan,Yan Huang,Jun Cai

Computational and mathematical methods in medicine 2022:9897669 PubMed36164617

2022

-Elemene Improves Morphine Tolerance in Bone Cancer Pain via N-Methyl-D-Aspartate Receptor 2B Subunit-Mediated -Opioid Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Qinfei Zhou,Liyan Gong,Guanai Bao,Qunfang Ding,Jingjing Ji
View all publications
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Product promise

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