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AB275096

Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free

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Rabbit Recombinant Monoclonal NMDAR2B antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.

View Alternative Names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

7 Images
Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • WB

Lab

Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Bands of 180KDa and 115KDa respectively represent the full length and endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID : 22523092)

Negative control : Liver (PMID : 10049997, 9712644).

Exposure time : 10 seconds.

Lanes 1, 2, 4 and 5:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (<a href='/en-us/products/primary-antibodies/nmdar2b-antibody-epr23460-119-ab254356'>ab254356</a>) at 1/2000 dilution

Lane 3:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (<a href='/en-us/products/primary-antibodies/nmdar2b-antibody-epr23460-119-ab254356'>ab254356</a>) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 10 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Lane 4:

Rat hippocampus tissue lysate at 10 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the mouse hippocampus (PMID : 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human cerebrum (PMID : 7931566). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NMDAR2B with ab254356 at 1/1000 (0.514 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the rat hippocampus (PMID : 7931566). The section was incubated with ab254356 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • IP

Unknown

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

NMDAR2B was immunoprecipitated from 0.35 mg rat brain tissue lysate 10 μg with ab254356 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 μg.

Lane 2 : ab254356 IP in rat brain tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254356 in rat brain tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

All lanes:

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (<a href='/en-us/products/primary-antibodies/nmdar2b-antibody-epr23460-119-ab254356'>ab254356</a>)

Predicted band size: 166 kDa

false

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • IP

Unknown

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

NMDAR2B was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 μg with ab254356 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 μg.

Lane 2 : ab254356 IP in mouse brain tissue lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254356 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Sample loaded onto lane 1 was non-boiled as boiling may cause protein aggregates. The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

All lanes:

Immunoprecipitation - Anti-NMDAR2B antibody [EPR23460-119] (<a href='/en-us/products/primary-antibodies/nmdar2b-antibody-epr23460-119-ab254356'>ab254356</a>)

Predicted band size: 166 kDa

false

Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)
  • WB

Lab

Western blot - Anti-NMDAR2B antibody [EPR23460-119] - BSA and Azide free (AB275096)

This data was developed using ab254356, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Bands of 180KDa, 150KDa and 115KDa respectively represent the full length and endogenously cleaved form.

The expression profile observed is consistent with what has been described in the literature (PMID : 22523092).

Exposure time : 48 seconds.

All lanes:

Western blot - Anti-NMDAR2B antibody [EPR23460-119] (<a href='/en-us/products/primary-antibodies/nmdar2b-antibody-epr23460-119-ab254356'>ab254356</a>) at 1/1000 dilution

All lanes:

Human hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/100000 dilution

Predicted band size: 166 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23460-119

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab275096 is the carrier-free version of ab254356.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.
Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
See full target information GRIN2B
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Product promise

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For full details, please see our Terms & Conditions

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