Anti-NMDAR2B antibody [NR2B]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [NR2B] (AB28373)

ab28373 staining NMDAR2B in Human breast tissue sections (right) compared with negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a sodium citrate buffer. Samples were incubated with primary antibody (1/20) overnight at 4°C. A HRP-conjugated secondary antibody was used for detection.

• Flow Cyt

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Flow Cytometry - Anti-NMDAR2B antibody [NR2B] (AB28373)

Overlay histogram showing SH-SY5Y cells stained with ab28373 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28373, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

AbReview27255****

Western blot - Anti-NMDAR2B antibody [NR2B] (AB28373)

Samples were incubated with primary antibody for 12 hours at 4^°C.

Blocked with 5% milk for 1 hour at 22^°C.

All lanes:

Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution

All lanes:

Rat P14 Cortex Lysate, vesicular fraction at 5 µg

Secondary

All lanes:

HRP conjugated Donkey anti-mouse IgG at 1/15000 dilution

Predicted band size: 166 kDa

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This image is courtesy of an anonymous Abreview

• WB

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Western blot - Anti-NMDAR2B antibody [NR2B] (AB28373)

Lanes 2 - 4:

Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution

Lanes 5 - 7:

Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution

Lanes 3 and 6:

Mouse brain (50ug)

Lanes 4 and 7:

Rat brain (50ug)

Predicted band size: 166 kDa

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• WB

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Western blot - Anti-NMDAR2B antibody [NR2B] (AB28373)

All lanes:

Western blot - Anti-NMDAR2B antibody [NR2B] (ab28373) at 2 µg/mL

All lanes:

mouse brain tissue

Predicted band size: 166 kDa

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