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Mouse Recombinant Monoclonal NMDAR2B antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Grin2b aa 1-300.


Images

Flow Cytometry - Anti-NMDAR2B antibody [S59] (AB93610), expandable thumbnail
  • Western blot - Anti-NMDAR2B antibody [S59] (AB93610), expandable thumbnail
  • Western blot - Anti-NMDAR2B antibody [S59] (AB93610), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [S59] (AB93610), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (AB93610), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Rat Grin2b aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q00960

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Expected
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected
Expected
Dog
Predicted
Predicted
Predicted
Predicted
Predicted

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1-10 µg/mL
Notes

-

Species
Human
Dilution info
1-10 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Dog
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:24272827, PubMed:24863970, PubMed:26875626, PubMed:26919761, PubMed:27839871, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:24272827, PubMed:24863970, PubMed:26875626, PubMed:26919761, PubMed:27839871, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

Alternative names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

Recommended products

Mouse Recombinant Monoclonal NMDAR2B antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Grin2b aa 1-300.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Rat Grin2b aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q00960
Clone number
S59
Purification technique
Affinity purification Protein G
Specificity

No cross-reactivity with NMDAR2A.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from S59-36 to N59/36, both clone numbers name the same antibody clone.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.

Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Associated diseases and disorders

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-NMDAR2B antibody [S59] (ab93610), expandable thumbnail

    Flow Cytometry - Anti-NMDAR2B antibody [S59] (ab93610)

    Overlay histogram showing SH-SY5Y cells stained with ab93610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93610, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback

  • Western blot - Anti-NMDAR2B antibody [S59] (ab93610), expandable thumbnail

    Western blot - Anti-NMDAR2B antibody [S59] (ab93610)

    All lanes: Western blot - Anti-NMDAR2B antibody [S59] (ab93610) at 1/250 dilution

    Lane 1: Induced HEK-T lysates

    Lane 2: Non-Induced HEK-T lysates

    Lane 3: P23X HEK-T negative control lysates

    Predicted band size: 166 kDa

  • Western blot - Anti-NMDAR2B antibody [S59] (ab93610), expandable thumbnail

    Western blot - Anti-NMDAR2B antibody [S59] (ab93610)

    Blocking buffer: 1.5% BSA for 30 minutes at RT.

    All lanes: Western blot - Anti-NMDAR2B antibody [S59] (ab93610) at 1/1000 dilution

    All lanes: Rat brain membrane lysate at 15 µg with 1.5% BSA for 30 minutes at RT

    Secondary

    All lanes: Sheep Anti-Mouse IgG: HRP for 1 hour at RT

    Predicted band size: 166 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [S59] (ab93610), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2B antibody [S59] (ab93610)

    Immunohistochemistry analysis of Bouin’s Fixed and paraffin-embedded Mouse backskin tissue using ab93610 at 1/100 dilutioon. The section was incubated with ab93610 for 1 hour at RT followed by FITC Goat Anti-Mouse secondary antibody at 1/50 dilution for 1 hour at RT (green). Positive Filaggrin-like staining and dermal staining observed with mouse backskin tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (ab93610), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody [S59] (ab93610)

    Immunocytochemistry/Immunofluorescence analysisi of 4% PFA fixed Human Neuroblastoma cells (SH-SY5Y) using ab93610 at 1/50 dilution. The section was incubated with ab93610 overnight at 4°C with slow rocking followed by AlexaFluor 488 secindary antibody at 1/100 dilution for 1 hour at RT (Green). Hoechst was used as nuclear stain (A) and Phalloidin-iFluor 647 (red) was used as actin counterstaining (B).

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Product protocols

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