Rabbit Polyclonal NMDAR2B phospho S1166 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Rat Grin2b phospho S1166.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Rat Grin2b phospho S1166. The exact immunogen used to generate this antibody is proprietary information. Database link Q00960
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Dog | Predicted |
| Primates | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Dog, Human, Xenopus laevis, Primates, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:11929923, PubMed:19910922, PubMed:21677647, PubMed:24607230, PubMed:24876489, PubMed:27135925, PubMed:7524561). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:11929923, PubMed:19910922, PubMed:21677647, PubMed:24607230, PubMed:24876489, PubMed:27135925, PubMed:7524561). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:10436042, PubMed:11929923, PubMed:24607230, PubMed:9463421). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage (By similarity). Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
Alternative names
GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b
Recommended products
Rabbit Polyclonal NMDAR2B phospho S1166 antibody. Suitable for WB and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Rat Grin2b phospho S1166.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.5
Constituents: HEPES, 50% Glycerol (glycerin, glycerine), 10% BSA, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Rat Grin2b phospho S1166. The exact immunogen used to generate this antibody is proprietary information. Database link Q00960
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.
- Biological function summary
The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.
- Pathways
NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.
- Associated diseases and disorders
Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-NMDAR2B (phospho S1166) antibody (ab254092)
All lanes: Western blot - Anti-NMDAR2B (phospho S1166) antibody (ab254092) at 1/250 dilution
Lane 1: Rat hippocampal lysate
Lane 2: Rat hippocampal lysate with immunizing phosphopeptide
Predicted band size: 166 kDa
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