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AB81271

Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]

5

(1 Review)

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(34 Publications)

Rabbit Recombinant Monoclonal NMDAR2B phospho S1303 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Rat, Human samples. Cited in 34 publications.

View Alternative Names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

4 Images
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)
  • WB

Unknown

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

Lane 1:

Human brain tissue lysate at 10 µg

Lane 2:

Human brain tissue lysate. The membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 180 kDa

false

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)
  • WB

Unknown

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

Lane 1:

Rat brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 166 kDa

Observed band size: 180 kDa

false

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)
  • WB

Unknown

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)

All lanes:

Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

Lane 1:

Mouse brain lysates (untreated) at 10 µg

Lane 2:

Mouse brain lysates (treated with AP) at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

Predicted band size: 166 kDa

Observed band size: 180 kDa

false

Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)
  • Dot

Unknown

Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (AB81271)

Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST.

  • Carrier free

    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1858Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.
Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
See full target information GRIN2B phospho S1303
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 25:81 PubMed38760739

2024

Alleviation of migraine related pain and anxiety by inhibiting calcium-stimulating AC1-dependent CGRP in the insula of adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Chenhao Li,Qi-Yu Chen,Shun Hao,Jingrui Mao,Wenwen Zhang,Xun Han,Zhao Dong,Ruozhuo Liu,Wenjing Tang,Min Zhuo,Shengyuan Yu,Yinglu Liu

The Journal of international medical research 52:3000605241235589 PubMed38546233

2024

Hydrogen treatment reduces electroencephalographic activity and neuronal death in rats with refractory status epilepticus by inhibiting membrane NR2B phosphorylation and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ruihua Jia,Gemin Zhu,Rui Zhao,Tian Li,Wen Jiang,Xiaoli Cui

PloS one 18:e0284626 PubMed37216352

2023

Round-window delivery of lithium chloride regenerates cochlear synapses damaged by noise-induced excitotoxic trauma via inhibition of the NMDA receptor in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Eun Choi,Nathaniel T Carpena,Jae-Hun Lee,So-Young Chang,Min Young Lee,Jae Yun Jung,Won-Ho Chung

Acta pharmaceutica Sinica. B 13:3708-3727 PubMed37719378

2023

Gestational dexamethasone exposure impacts hippocampal excitatory synaptic transmission and learning and memory function with transgenerational effects.

Applications

Unspecified application

Species

Unspecified reactive species

Mingcui Luo,Yiwen Yi,Songqiang Huang,Shiyun Dai,Lulu Xie,Kexin Liu,Shuai Zhang,Tao Jiang,Tingting Wang,Baozhen Yao,Hui Wang,Dan Xu

Oxidative medicine and cellular longevity 2022:4661519 PubMed36439691

2022

BHF177 Suppresses Diabetic Neuropathic Pain by Blocking PKC/CaMKII/ERK1/2/CREB Signaling Pathway through Activating GABA Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Boyu Liu,Fengxi Guan,Jiapeng Zhao,Yao Niu,Hongbo Jiang

Current research in toxicology 3:100093 PubMed36393872

2022

Developmental deltamethrin: Sex-specific hippocampal effects in Sprague Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Emily M Pitzer,Chiho Sugimoto,Samantha L Regan,Gary A Gudelsky,Michael T Williams,Charles V Vorhees

Frontiers in cellular neuroscience 16:911973 PubMed35928572

2022

Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Sun,Qiyao Li,Xiaopeng Li,Yunpeng Shi,Chengrui Nan,Qianxu Jin,Xiaoyan Wang,Yayu Zhuo,Zongmao Zhao

Frontiers in endocrinology 13:887238 PubMed35712239

2022

Activation of GIPR Exerts Analgesic and Anxiolytic-Like Effects in the Anterior Cingulate Cortex of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Shang Wang,Yong-Li Jiang,Liang Lu,Ban Feng,Xue Ma,Kun Zhang,Shao-Yu Guan,Le Yang,Qing-Yu Fan,Xiao-Chen Zhu,Fan Yang,Jing-Yu Qi,Liu-Kun Yang,Xu-Bo Li,Ming-Gao Zhao,Wen Jiang,Zhen Tian,Shui-Bing Liu

Neurobiology of disease 158:105456 PubMed34352385

2021

A novel role for the ADHD risk gene latrophilin-3 in learning and memory in Lphn3 knockout rats.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha L Regan,Emily M Pitzer,Jillian R Hufgard,Chiho Sugimoto,Michael T Williams,Charles V Vorhees

Frontiers in cell and developmental biology 9:678182 PubMed34336832

2021

Requirements of Postnatal proBDNF in the Hippocampus for Spatial Memory Consolidation and Neural Function.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Sun,Hong Cheng,Yang Yang,Dongxin Tang,Xiaolian Li,Lei An
View all publications
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