Rabbit Polyclonal NMDAR2B phospho S1480 antibody. Suitable for WB and reacts with Rat samples. Cited in 5 publications.
Images
Publications
- Frontiers in cellular neuroscience 16:9119732022Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Sun,Qiyao Li,Xiaopeng Li,Yunpeng Shi,Chengrui Nan,Qianxu Jin,Xiaoyan Wang,Yayu Zhuo,Zongmao ZhaoPubMed 35928572
- Communications biology 4:11382021SALM4 negatively regulates NMDA receptor function and fear memory consolidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunkyung Lie,Yeji Yeo,Eun-Jae Lee,Wangyong Shin,Kyungdeok Kim,Kyung Ah Han,Esther Yang,Tae-Yong Choi,Mihyun Bae,Suho Lee,Seung Min Um,Se-Young Choi,Hyun Kim,Jaewon Ko,Eunjoon KimPubMed 34588597
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q00960
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Chicken | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Primates | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Cow, Dog, Human, Primates, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1350383, PubMed:19910922, PubMed:21677647, PubMed:24876489, PubMed:27135925, PubMed:27916457). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).
Alternative names
GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B
Recommended products
Rabbit Polyclonal NMDAR2B phospho S1480 antibody. Suitable for WB and reacts with Rat samples. Cited in 5 publications.
Alternative names
GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.5
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.238% HEPES, 0.01% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q00960
- Purification technique
Affinity purification Immunogen
- Specificity
ab73014 is specific for ~180k NMDAR2B protein phosphorylated at Ser1480. Immunolabeling of the NMDAR2B band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment.
- Concentration
- Purification notes
ab73014 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-NMDAR2B (phospho S1480) antibody (ab73014)
The phosphospecificity of the labeling is shown in the second lane (lambda-phosphatase). The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
All lanes: Western blot - Anti-NMDAR2B (phospho S1480) antibody (ab73014) at 1/1000 dilution
Lane 1: Rat hippocampal lysate
Lane 2: Rat hippocampal lysate incubated in lambda-phosphatase (1200 units for 30 min)
Predicted band size: 166 kDa
Observed band size: 180 kDa, 85 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com