AB18532

Anti-NMDAR2B (phospho Y1252) antibody

(3 Reviews)

(5 Publications)

Rabbit Polyclonal NMDAR2B phospho Y1252 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3

2 Images

Lab

Western blot - Anti-NMDAR2B (phospho Y1252) antibody (AB18532)

All lanes:

ab18532 at 1 mg/mL

Lane 1:

Mouse Brain Tissue Lysate at 20 µg

Lane 2:

Mouse Fore-brain Tissue Lysate at 20 µg

Lane 3:

Mouse Cortex Tissue Lysate at 20 µg

Lane 4:

Mouse Cerebellum Tissue Lysate at 20 µg

Lane 5:

Mouse Hippo-campus Tissue Lysate at 20 µg

Lane 6:

Human Brain Tissue Lysate at 20 µg

Lane 7:

Human Cortex Tissue Lysate at 20 µg

Lane 8:

Human Cerebellum Tissue Lysate at 20 µg

Lane 9:

Human Hippo-campus Tissue Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa,18 kDa,45 kDa,60 kDa

true

Exposure time: 20min

Collaborator

Western blot - Anti-NMDAR2B (phospho Y1252) antibody (AB18532)

Lanes 1 - 3:

Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/mL

Lane 4:

NR2B antibody (positive control)

Lane 1:

mouse cortical extract at 20 µg with Mouse NMDAR2B (unmodified ) peptide (<a href='/en-us/products/unavailable/mouse-nmdar2b-unmodified-peptide-ab19637'>ab19637</a>)

Lane 2:

mouse cortical extract at 20 µg with Mouse NMDAR2B (phospho Y1252) peptide (<a href='/en-us/products/unavailable/mouse-nmdar2b-phospho-y1252-peptide-ab19636'>ab19636</a>)

Lanes 3 - 4:

mouse cortical extract at 20 µg

Predicted band size: 166 kDa

Observed band size: 115 kDa,166 kDa

false

This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.

Biological function summary

The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.

Pathways

NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.

Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed : 24272827, PubMed : 24863970, PubMed : 26875626, PubMed : 26919761, PubMed : 27839871, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed : 26875626, PubMed : 28095420, PubMed : 28126851, PubMed : 38538865, PubMed : 8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).

See full target information GRIN2B phospho Y1252

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Synapse (New York, N.Y.) 77:e22250 PubMed36085433

2022

Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam E Reyes-Méndez,J Manuel Herrera-Zamora,Fernando Osuna-Lopez,Irving S Aguilar-Martínez,José L Góngora-Alfaro,Ricardo A Navarro-Polanco,Enrique Sánchez-Pastor,Eloy G Moreno-Galindo,Javier Alamilla

Brain, behavior, and immunity 87:579-590 PubMed32032782

2020

N-methyl-d-aspartate receptor subunit 2B on keratinocyte mediates peripheral and central sensitization in chronic post-ischemic pain in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Xu,Xin Tao,Ping Huang,Feng Lin,Qing Liu,Li Xu,Jijun Xu,Yuguang Huang

Journal of biological rhythms 34:39-50 PubMed30654688

2019

Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat.

Applications

Unspecified application

Species

Unspecified reactive species

J Manuel Herrera-Zamora,Luis A Castro-Sánchez,Miriam Reyes-Mendez,Irving Aguilar-Martinez,Fernando Osuna-López,Eloy G Moreno-Galindo,Ricardo A Navarro-Polanco,Raul A Aguilar-Roblero,Enrique Sánchez-Pastor,Javier Alamilla

The international journal of neuropsychopharmacology 21:938-948 PubMed29860313

2018

Sigma 1 Receptor Antagonists Inhibit Manic-Like Behaviors in Two Congenital Strains of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pilar Sánchez-Blázquez,Elsa Cortés-Montero,María Rodríguez-Muñoz,Javier Garzón

The Journal of neuroscience : the official journal of the Society for Neuroscience 30:8162-70 PubMed20554866

2010

In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93.

Applications

Unspecified application

Species

Unspecified reactive species

Ilse Delint-Ramirez,Esperanza Fernández,Alex Bayés,Emese Kicsi,Noboru H Komiyama,Seth G N Grant

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NMDAR2B (phospho Y1252) antibody

Western blot - Anti-NMDAR2B (phospho Y1252) antibody (AB18532)

Western blot - Anti-NMDAR2B (phospho Y1252) antibody (AB18532)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise