Rabbit Polyclonal NMDAR2B phospho Y1252 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.
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- Synapse (New York, N.Y.) 77:e222502022Developmental light deprivation transiently reduces the expression of vGluT2 and GluN2B in the rat ventral suprachiasmatic nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiriam E Reyes-Méndez,J Manuel Herrera-Zamora,Fernando Osuna-Lopez,Irving S Aguilar-Martínez,José L Góngora-Alfaro,Ricardo A Navarro-Polanco,Enrique Sánchez-Pastor,Eloy G Moreno-Galindo,Javier AlamillaPubMed 36085433
- Journal of biological rhythms 34:39-502019Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Manuel Herrera-Zamora et. al.PubMed 30654688
- The international journal of neuropsychopharmacology 21:938-9482018Sigma 1 Receptor Antagonists Inhibit Manic-Like Behaviors in Two Congenital Strains of Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPilar Sánchez-Blázquez,Elsa Cortés-Montero,María Rodríguez-Muñoz,Javier GarzónPubMed 29860313
- The Journal of neuroscience : the official journal of the Society for Neuroscience 30:8162-702010In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlse Delint-Ramirez,Esperanza Fernández,Alex Bayés,Emese Kicsi,Noboru H Komiyama,Seth G N GrantPubMed 20554866
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Expected |
| Mouse | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:24272827, PubMed:24863970, PubMed:26875626, PubMed:26919761, PubMed:27839871, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:24272827, PubMed:24863970, PubMed:26875626, PubMed:26919761, PubMed:27839871, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). NMDARs mediate simultaneously the potasium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626, PubMed:28095420, PubMed:28126851, PubMed:38538865, PubMed:8768735). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity).
Alternative names
NMDAR2B, GRIN2B, GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3, hNR3
Recommended products
Rabbit Polyclonal NMDAR2B phospho Y1252 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMDAR2B also known as NR2B or GluN2B functions as a subunit of the NMDA receptor complex. It plays a role in synaptic transmission and plasticity in the central nervous system. The target weighs approximately 166 kDa. It is highly expressed in the cerebral cortex hippocampus and striatum. NMDAR2B interacts with other subunits in the NMDA receptor which assemble to form a functional ion channel that allows for calcium ion influx when activated by glutamate and glycine.
- Biological function summary
The NMDAR2B subunit contributes to the regulation of synaptic strength and is essential for processes involved in learning and memory. As part of the NMDA receptor complex it mediates excitatory neurotransmission and is involved in synaptic plasticity processes such as long-term potentiation (LTP). These functions are significant for cognitive function and neural development. The receptor's role in signal transduction is aided by the unique properties conferred by the NMDAR2B subunit such as its high affinity for glycine and slower deactivation kinetics.
- Pathways
NMDAR2B is involved in the glutamatergic signaling pathway which is important for neural communication. It also participates in the calcium signaling pathway affecting cellular responses to external stimuli. The protein interacts with CaMKII and PSD-95 which are proteins that influence synaptic strength and architecture through these pathways. Its involvement links it to a variety of signaling events important for brain function.
- Associated diseases and disorders
Alterations in NMDAR2B have been associated with neurodegenerative conditions such as Alzheimer's disease and neurodevelopmental disorders like schizophrenia. The protein's malfunction can lead to abnormal synaptic connectivity and excitotoxicity. It is linked to other proteins associated with these diseases such as beta-amyloid in Alzheimer's and dopamine receptor dysregulation in schizophrenia indicating its role in disease progression and symptom manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)
All lanes: ab18532 at 1 mg/mL
Lane 1: Mouse Brain Tissue Lysate at 20 µg
Lane 2: Mouse Fore-brain Tissue Lysate at 20 µg
Lane 3: Mouse Cortex Tissue Lysate at 20 µg
Lane 4: Mouse Cerebellum Tissue Lysate at 20 µg
Lane 5: Mouse Hippo-campus Tissue Lysate at 20 µg
Lane 6: Human Brain Tissue Lysate at 20 µg
Lane 7: Human Cortex Tissue Lysate at 20 µg
Lane 8: Human Cerebellum Tissue Lysate at 20 µg
Lane 9: Human Hippo-campus Tissue Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 166 kDa, 18 kDa, 45 kDa, 60 kDa
Exposure time: 20min
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United KingdomWestern blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)
Lanes 1 - 3: Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/mL
Lane 4: NR2B antibody (positive control)
Lane 1: mouse cortical extract at 20 µg with Mouse NMDAR2B (unmodified ) peptide (ab19637)
Lane 2: mouse cortical extract at 20 µg with Mouse NMDAR2B (phospho Y1252) peptide (ab19636)
Lanes 3 - 4: mouse cortical extract at 20 µg
Predicted band size: 166 kDa
Observed band size: 115 kDa, 166 kDa
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