AB204958

Anti-NME2 antibody [4G7A8]

Name: Anti-NME2 antibody [4G7A8] (ab204958) | Abcam
Brand: Abcam Limited
SKU: AB204958

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(2 Publications)

Mouse Monoclonal NME2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NME2.

View Alternative Names

NDK2, NM23B, NME2, Nucleoside diphosphate kinase B, NDP kinase B, NDPK B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, Nucleoside diphoshate kinase 2, nm23-H2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (AB204958)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (AB204958)

Immunohistochemical analysis of paraffin-embedded, DAB-stained human ovarian cancer tissue, labeling NME2 using ab204958 at a 1/200 dilution

Supplier Data

Western blot - Anti-NME2 antibody [4G7A8] (AB204958)

(Expected MW is 43.2 kDa)

All lanes:

Western blot - Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution

All lanes:

Human full length recombinant NME2 (amino acids 1-152).

Predicted band size: 17 kDa

false

Supplier Data

Western blot - Anti-NME2 antibody [4G7A8] (AB204958)

All lanes:

Western blot - Anti-NME2 antibody [4G7A8] (ab204958) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Lysate from HEK293 cells transfected with full length recombinant NME2 (amino acids 1-152).

Predicted band size: 17 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4G7A8

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NME2.

P22392

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NME2 also known as NM23-H2 is a nucleoside diphosphate kinase that is involved in the synthesis of nucleoside triphosphates. With a mass of approximately 17 kDa it plays a role in the energetic metabolism of cells. NME2 is widely expressed in various tissues including the liver heart and brain. Its expression levels indicate its importance in maintaining regular cellular functions.

Biological function summary

NME2 participates in the regulation of transcription and cellular differentiation. It acts within the context of protein complexes which influence DNA-binding activities required for transcriptional control. NME2 also has roles in the regulation of cell proliferation differentiation and signaling. These activities demonstrate the protein's significance in maintaining normal physiological processes.

Pathways

NME2 is active in the MAPK/ERK signaling pathway significant in cell growth and differentiation. It interacts with other proteins like ERK2 to modulate downstream signaling cascades. This protein also features in the GTP nucleotide biosynthesis pathway where it cooperates with other kinases for nucleotide metabolism highlighting its important role in cell cycle regulation.

Mutations or altered expression of NME2 link with various cancers including breast and colon cancer. This connection emphasizes its potential role as a biomarker or therapeutic target. The protein also associates with other oncogenes like NME1 together contributing to the metastatic potential of cancer cells. NME2's involvement in chronic conditions like cancer confirms its importance in pathological states where it may influence disease progression or serve as a clinical marker.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 11121025, PubMed : 16313181, PubMed : 1851158, PubMed : 25679041). Also functions as a histidine protein kinase by transferring the phosphoryl group from the phosphohistidine intermediate to a histidine residue in target proteins (PubMed : 17157250, PubMed : 20946858). Phosphorylates the GNB1 subunit of heterotrimeric G proteins at 'His-266', generating a high-energy phosphate group that promotes GTP formation and enables receptor-independent activation of heterotrimeric G proteins (By similarity). Also phosphorylates KCNN4 at 'His-358', leading to activation of its intermediate conductance calcium-activated potassium channel activity, Ca(2+) influx, and subsequent activation of B and T cells (PubMed : 17157250). Additionally involved in transcriptional regulation through direct DNA binding and chromatin remodeling (PubMed : 11121025, PubMed : 11694515, PubMed : 19033359, PubMed : 19435876, PubMed : 25679041, PubMed : 8392752). In this context, functions as a single-stranded DNA binding protein that binds and stabilizes the G-quadruplex (G4) structures within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter, facilitating recruitment of additional single-strand DNA binding proteins and activation of MYC transcription (PubMed : 19033359, PubMed : 19435876, PubMed : 25679041, PubMed : 8392752). G4 DNA-binding activity is independent of its nucleoside diphosphate kinase function and recognizes both folded and unfolded G4 structures (PubMed : 25679041). With NME1, may regulate acetyl-CoA (AcCoA) usage between histone acetylation and fatty acid synthesis by targeting AcCoA release at ATP-rich, HAT-associated chromatin regions (By similarity). Also negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed : 15249197).

See full target information NME2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:352 PubMed38191557

2024

Mechanism-centric regulatory network identifies NME2 and MYC programs as markers of Enzalutamide resistance in CRPC.

Applications

Unspecified application

Species

Unspecified reactive species

Sukanya Panja,Mihai Ioan Truica,Christina Y Yu,Vamshi Saggurthi,Michael W Craige,Katie Whitehead,Mayra V Tuiche,Aymen Al-Saadi,Riddhi Vyas,Shridar Ganesan,Suril Gohel,Frederick Coffman,James S Parrott,Songhua Quan,Shantenu Jha,Isaac Kim,Edward Schaeffer,Vishal Kothari,Sarki A Abdulkadir,Antonina Mitrofanova

Cells 11: PubMed35406727

2022

Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Ferrari,Bowen Xie,Edwyn Assaf,Kristin Morder,Melanie Scott,Hong Liao,Michael J Calderon,Mark Ross,Patricia Loughran,Simon C Watkins,Iraklis Pipinos,George Casale,Edith Tzeng,Ryan McEnaney,Ulka Sachdev

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NME2 antibody [4G7A8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [4G7A8] (AB204958)

Western blot - Anti-NME2 antibody [4G7A8] (AB204958)

Western blot - Anti-NME2 antibody [4G7A8] (AB204958)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise