Mouse Monoclonal NME2 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NME2.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89613.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210)")
![Protein Array - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--protein-array-img2006.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210)")
![Western blot - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--western-blot-img41292.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (AB268210)")
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NME2. Database link P22392
Reactivity data
Select an application| WB | Protein Array | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
4 products for Alternative Product
Target data
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197). Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752, PubMed:19435876). Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1) (PubMed:19435876). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041). Exhibits histidine protein kinase activity (PubMed:20946858).
Alternative names
Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2, NME2, NM23B
Recommended products
Mouse Monoclonal NME2 antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NME2.
Alternative names
Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2, NME2, NM23B
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NME2. Database link P22392
- Carrier free
Yes
- Clone number
CPTC-NME2-2
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
Purified from Bioreactor concentrate
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab268210 is a carrier free version of Anti-NME2 antibody [CPTC-NME2-2] ab268089.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NME2 also known as NM23-H2 is a nucleoside diphosphate kinase that is involved in the synthesis of nucleoside triphosphates. With a mass of approximately 17 kDa it plays a role in the energetic metabolism of cells. NME2 is widely expressed in various tissues including the liver heart and brain. Its expression levels indicate its importance in maintaining regular cellular functions.
- Biological function summary
NME2 participates in the regulation of transcription and cellular differentiation. It acts within the context of protein complexes which influence DNA-binding activities required for transcriptional control. NME2 also has roles in the regulation of cell proliferation differentiation and signaling. These activities demonstrate the protein's significance in maintaining normal physiological processes.
- Pathways
NME2 is active in the MAPK/ERK signaling pathway significant in cell growth and differentiation. It interacts with other proteins like ERK2 to modulate downstream signaling cascades. This protein also features in the GTP nucleotide biosynthesis pathway where it cooperates with other kinases for nucleotide metabolism highlighting its important role in cell cycle regulation.
- Associated diseases and disorders
Mutations or altered expression of NME2 link with various cancers including breast and colon cancer. This connection emphasizes its potential role as a biomarker or therapeutic target. The protein also associates with other oncogenes like NME1 together contributing to the metastatic potential of cancer cells. NME2's involvement in chronic conditions like cancer confirms its importance in pathological states where it may influence disease progression or serve as a clinical marker.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89613.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NME2 using Anti-NME2 antibody [CPTC-NME2-2] ab268089 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-NME2 antibody [CPTC-NME2-2] ab268089).
![Protein Array - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--protein-array-img2006.jpg "Protein Array - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)")
Protein Array - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)
Protein Array containing more than 19,000 full-length human proteins using Anti-NME2 antibody [CPTC-NME2-2] ab268089. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-NME2 antibody [CPTC-NME2-2] ab268089).
![Western blot - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210), expandable thumbnail](https://content.abcam.com/products/images/nme2-antibody-cptc-nme2-2-bsa-and-azide-free-ab268210--western-blot-img41292.jpg "Western blot - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)")
Western blot - Anti-NME2 antibody [CPTC-NME2-2] - BSA and Azide free (ab268210)
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-NME2 antibody [CPTC-NME2-2] ab268089)
All lanes: Western blot - Anti-NME2 antibody [CPTC-NME2-2] (Anti-NME2 antibody [CPTC-NME2-2] ab268089) at 2 µg/mL
All lanes: PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Predicted band size: 17 kDa
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