AB181257

Anti-NME3 antibody [EPR13117]

Name: Anti-NME3 antibody [EPR13117] (ab181257) | Abcam
Brand: Abcam Limited
SKU: AB181257
Price: 485 USD
Availability: InStock
Rating: 1 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal NME3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (AB181257)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (AB181257)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (AB181257)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-NME3 antibody [EPR13117] (AB181257)

Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NME3 antibody [EPR13117] (ab181257)

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-NME3 antibody [EPR13117] (AB181257)

All lanes:

Western blot - Anti-NME3 antibody [EPR13117] (ab181257) at 1/2000 dilution

Lane 1:

MOLT4 cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

false

Carrier free

Anti-NME3 antibody [EPR13117] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13117

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/170", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NME3 also known as non-metastatic cells protein 3 is a part of the nucleoside diphosphate kinase (NDPK) family. This protein has a molecular mass of approximately 17.3 kDa. Scientists have reported its expression in various tissues with particular abundance in lungs and brain. NME3 interacts with nucleotides participating in phosphate transfer reactions by converting nucleoside diphosphates into nucleoside triphosphates.

Biological function summary

Non-metastatic cells protein 3 plays a role in maintaining cellular nucleotide homeostasis. NME3 is a member of a larger NDPK complex working closely with other NDPK family members like NME1 and NME2. This complex contributes to DNA replication and repair processes. It also influences cell proliferation and differentiation by regulating the levels of GTP and other nucleotides important for these biological processes.

Pathways

There is significant involvement of non-metastatic cells protein 3 in cellular energy metabolism and signal transduction pathways. NME3 participates in the Ras signaling pathway where it interacts with Ras proteins regulating their activation state. Additionally NME3 functions within the Wnt signaling pathway contributing to cell cycle regulation and development.

Non-metastatic cells protein 3 associates with cancer where altered NME3 expression correlates with tumor progression and metastasis. Studies indicate that changes in NME3 can disrupt interactions with proteins like NME1 influencing cancer cell motility and invasion. NME3 also connects with neurodegenerative disorders with dysregulation possibly affecting cellular processes that protect against neuronal cell death.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 11277919, PubMed : 30587587, PubMed : 39337255). In vitro, can also use other phosphate donors such as UTP and GTP (PubMed : 30587587, PubMed : 39337255). Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization (PubMed : 30587587, PubMed : 37584589). Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity (PubMed : 26945015). Inhibits granulocyte differentiation (PubMed : 7638209). May be required for ciliary function during renal development (By similarity).

See full target information Nucleoside diphosphate kinase 3

Alternative Names

Nucleoside diphosphate kinase C, NDPK-C, DR-nm23, Nucleoside diphosphate kinase 3, nm23-H3, NDK3, NME3

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Aging cell 20:e13308 PubMed33480139

2021

Incomplete proline catabolism drives premature sperm aging.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-An Yen,Sean P Curran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NME3 antibody [EPR13117]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (AB181257)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] (AB181257)

Immunoprecipitation - Anti-NME3 antibody [EPR13117] (AB181257)

Western blot - Anti-NME3 antibody [EPR13117] (AB181257)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise