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AB250470

Anti-NME3 antibody [EPR13117] - BSA and Azide free

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Rabbit Recombinant Monoclonal NME3 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

View Alternative Names

Nucleoside diphosphate kinase C, NDPK-C, DR-nm23, Nucleoside diphosphate kinase 3, nm23-H3, NDK3, NME3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)

This data was developed using ab181257, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)

This data was developed using ab181257, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NME3 with ab181257 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)
  • IP

Supplier Data

Immunoprecipitation - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)

This data was developed using ab181257, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from MOLT4 cell lysate immunoprecipitated using ab181257 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NME3 antibody [EPR13117] (<a href='/en-us/products/primary-antibodies/nme3-antibody-epr13117-ab181257'>ab181257</a>)

Predicted band size: 19 kDa

false

Western blot - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)
  • WB

Supplier Data

Western blot - Anti-NME3 antibody [EPR13117] - BSA and Azide free (AB250470)

This data was developed using ab181257, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NME3 antibody [EPR13117] (<a href='/en-us/products/primary-antibodies/nme3-antibody-epr13117-ab181257'>ab181257</a>) at 1/2000 dilution

Lane 1:

MOLT4 cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13117

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250470 is the carrier-free version of ab181257.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NME3 also known as non-metastatic cells protein 3 is a part of the nucleoside diphosphate kinase (NDPK) family. This protein has a molecular mass of approximately 17.3 kDa. Scientists have reported its expression in various tissues with particular abundance in lungs and brain. NME3 interacts with nucleotides participating in phosphate transfer reactions by converting nucleoside diphosphates into nucleoside triphosphates.
Biological function summary

Non-metastatic cells protein 3 plays a role in maintaining cellular nucleotide homeostasis. NME3 is a member of a larger NDPK complex working closely with other NDPK family members like NME1 and NME2. This complex contributes to DNA replication and repair processes. It also influences cell proliferation and differentiation by regulating the levels of GTP and other nucleotides important for these biological processes.

Pathways

There is significant involvement of non-metastatic cells protein 3 in cellular energy metabolism and signal transduction pathways. NME3 participates in the Ras signaling pathway where it interacts with Ras proteins regulating their activation state. Additionally NME3 functions within the Wnt signaling pathway contributing to cell cycle regulation and development.

Non-metastatic cells protein 3 associates with cancer where altered NME3 expression correlates with tumor progression and metastasis. Studies indicate that changes in NME3 can disrupt interactions with proteins like NME1 influencing cancer cell motility and invasion. NME3 also connects with neurodegenerative disorders with dysregulation possibly affecting cellular processes that protect against neuronal cell death.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 11277919, PubMed : 30587587, PubMed : 39337255). In vitro, can also use other phosphate donors such as UTP and GTP (PubMed : 30587587, PubMed : 39337255). Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization (PubMed : 30587587, PubMed : 37584589). Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity (PubMed : 26945015). Inhibits granulocyte differentiation (PubMed : 7638209). May be required for ciliary function during renal development (By similarity).
See full target information Nucleoside diphosphate kinase 3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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