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AB229772

Anti-Nmnat1/NMNAT antibody

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Rabbit Polyclonal Nmnat1/NMNAT antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NMNAT1.

View Alternative Names

NMNAT, NMNAT1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide-nucleotide adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, NMN adenylyltransferase 1, NaMN adenylyltransferase 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody (AB229772)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody (AB229772)

PC-3 (human prostate adenocarcinoma cell line) cells stained for Nmnat1/NMNAT (green) using ab229772 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nmnat1/NMNAT antibody (AB229772)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nmnat1/NMNAT antibody (AB229772)

Paraffin-embedded human kidney tissue stained for Nmnat1/NMNAT using ab229772 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NMNAT1.

Q9HAN9

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nmnat1 also known as nicotinamide mononucleotide adenylyltransferase 1 is an enzyme involved in the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+). This enzyme weighs approximately 32 kDa and is expressed largely in mammalian tissues with higher concentrations in the brain heart and liver. In the research community variations like 'nmn ireland' or 'elisa namn' may refer to the study tools or location of research related to NMNAT.
Biological function summary

NMNAT1 plays a role in NAD+ biosynthesis which is essential for energy metabolism and cellular functions. It acts as a NAD+ synthase and may not work alone but within larger enzymatic complexes that handle cellular redox reactions. In neurons the enzyme supports axonal survival which connects it to neurodegenerative health studies.

Pathways

This enzyme becomes critical in the NAD+ biosynthesis pathway interacting closely with proteins such as nicotinamide phosphoribosyltransferase (NAMPT) in the salvage pathway. This pathway is key in maintaining cellular energy balance and gene expression regulation. The enzyme along with these related proteins ensures the recycling and regulation of NAD+ within the cells.

NMNAT1 holds significance in conditions like axonopathy and certain retinal degenerations. Mutations in the NMNAT1 gene have been linked to Leber's congenital amaurosis a severe genetic eye disorder. Studies often focus on NMNAT1 alongside other proteins like sirtuins as these participate in mitigating the oxidative stress aspects involved in neurodegeneration and associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed : 17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed : 17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed : 17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed : 17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed : 17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed : 27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed : 27257257). Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed : 17402747). Protects against axonal degeneration following mechanical or toxic insults (By similarity). Neural protection does not correlate with cellular NAD(+) levels but may still require enzyme activity (By similarity).
See full target information NMNAT1
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Product promise

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