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Mouse Monoclonal Nmnat1/NMNAT antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1.

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Images

Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (AB118270), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (AB118270), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1. Database link Q9HAN9

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Expected
African green monkey
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

African green monkey

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

African green monkey

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults (By similarity).

Alternative names

Recommended products

Mouse Monoclonal Nmnat1/NMNAT antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1. Database link Q9HAN9
Clone number

OTI1F7

Purification technique

Affinity purification

Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Clone OTI1F7 (formerly 1F7).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nmnat1 also known as nicotinamide mononucleotide adenylyltransferase 1 is an enzyme involved in the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+). This enzyme weighs approximately 32 kDa and is expressed largely in mammalian tissues with higher concentrations in the brain heart and liver. In the research community variations like 'nmn ireland' or 'elisa namn' may refer to the study tools or location of research related to NMNAT.

Biological function summary

NMNAT1 plays a role in NAD+ biosynthesis which is essential for energy metabolism and cellular functions. It acts as a NAD+ synthase and may not work alone but within larger enzymatic complexes that handle cellular redox reactions. In neurons the enzyme supports axonal survival which connects it to neurodegenerative health studies.

Pathways

This enzyme becomes critical in the NAD+ biosynthesis pathway interacting closely with proteins such as nicotinamide phosphoribosyltransferase (NAMPT) in the salvage pathway. This pathway is key in maintaining cellular energy balance and gene expression regulation. The enzyme along with these related proteins ensures the recycling and regulation of NAD+ within the cells.

Associated diseases and disorders

NMNAT1 holds significance in conditions like axonopathy and certain retinal degenerations. Mutations in the NMNAT1 gene have been linked to Leber's congenital amaurosis a severe genetic eye disorder. Studies often focus on NMNAT1 alongside other proteins like sirtuins as these participate in mitigating the oxidative stress aspects involved in neurodegeneration and associated disorders.

Product promise

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2 product images

  • Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270), expandable thumbnail

    Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)

    All lanes: Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270) at 1/2000 dilution

    Lane 1: pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

    Lane 2: pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected HEK-293T cell lysate at 5 µg

    Predicted band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270)

    pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Nmnat1/NMNAT using ab118270 at a 1/100 dilution in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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