Mouse Monoclonal Nmnat1/NMNAT antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1.
IgG1
Mouse
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Expected |
African green monkey | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species African green monkey | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (PubMed:17402747). Can use triazofurin monophosphate (TrMP) as substrate (PubMed:17402747). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (PubMed:17402747). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (PubMed:17402747). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (PubMed:17402747). Protects against axonal degeneration following mechanical or toxic insults (By similarity).
NMNAT, NMNAT1, NMNAT, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide-nucleotide adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, NMN adenylyltransferase 1, NaMN adenylyltransferase 1
Mouse Monoclonal Nmnat1/NMNAT antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1.
IgG1
Mouse
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
OTI1F7
Affinity purification
Purified from cell culture supernatant by affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Clone OTI1F7 (formerly 1F7).
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This supplementary information is collated from multiple sources and compiled automatically.
Nmnat1 also known as nicotinamide mononucleotide adenylyltransferase 1 is an enzyme involved in the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+). This enzyme weighs approximately 32 kDa and is expressed largely in mammalian tissues with higher concentrations in the brain heart and liver. In the research community variations like 'nmn ireland' or 'elisa namn' may refer to the study tools or location of research related to NMNAT.
NMNAT1 plays a role in NAD+ biosynthesis which is essential for energy metabolism and cellular functions. It acts as a NAD+ synthase and may not work alone but within larger enzymatic complexes that handle cellular redox reactions. In neurons the enzyme supports axonal survival which connects it to neurodegenerative health studies.
This enzyme becomes critical in the NAD+ biosynthesis pathway interacting closely with proteins such as nicotinamide phosphoribosyltransferase (NAMPT) in the salvage pathway. This pathway is key in maintaining cellular energy balance and gene expression regulation. The enzyme along with these related proteins ensures the recycling and regulation of NAD+ within the cells.
NMNAT1 holds significance in conditions like axonopathy and certain retinal degenerations. Mutations in the NMNAT1 gene have been linked to Leber's congenital amaurosis a severe genetic eye disorder. Studies often focus on NMNAT1 alongside other proteins like sirtuins as these participate in mitigating the oxidative stress aspects involved in neurodegeneration and associated disorders.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-Nmnat1/NMNAT antibody [OTI1F7] (ab118270) at 1/2000 dilution
Lane 1: pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg
Lane 2: pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected HEK-293T cell lysate at 5 µg
Predicted band size: 31 kDa
pCMV6-ENTRY Nmnat1/NMNAT cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for Nmnat1/NMNAT using ab118270 at a 1/100 dilution in ICC/IF.
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