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AB230839

Anti-NMNAT3 antibody

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(1 Publication)

Rabbit Polyclonal NMNAT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NMNAT3 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FKSG76, NMNAT3, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3, NMN/NaMN adenylyltransferase 3, Nicotinamide-nucleotide adenylyltransferase 3, Nicotinate-nucleotide adenylyltransferase 3, Pyridine nucleotide adenylyltransferase 3, NMN adenylyltransferase 3, NaMN adenylyltransferase 3, PNAT-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMNAT3 antibody (AB230839)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMNAT3 antibody (AB230839)

Formalin-fixed, paraffin-embedded human liver tissue stained for NMNAT3 using ab230839 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-NMNAT3 antibody (AB230839)
  • WB

Supplier Data

Western blot - Anti-NMNAT3 antibody (AB230839)

All lanes:

Western blot - Anti-NMNAT3 antibody (ab230839) at 1/1000 dilution

All lanes:

NCI-H460 (human lung cancer cell line) cell lysate at 35 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NMNAT3 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96T66

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMNAT3 or nicotinamide nucleotide adenylyltransferase 3 catalyzes the biosynthesis of NAD+ from NMN and ATP playing a role in cellular energy metabolism. Alternate names include NMNAT3p and Enzyme NIC. It has a molecular mass of around 31 kDa. NMNAT3 is expressed in a variety of tissues with significant expression in red blood cells and the mitochondria of other cell types.
Biological function summary

NMNAT3 participates in the NAD+ salvage pathway transforming NMN into NAD+ a fundamental coenzyme in cellular metabolism and redox reactions. It does not form part of any larger complex but functions independently within the cell. Its activity supports energy production DNA repair and cell signaling processes making it an important player in maintaining cellular homeostasis.

Pathways

The salvage pathway for NAD+ biosynthesis relies heavily on NMNAT3's enzymatic action. It works alongside related proteins like NMNAT1 and NMNAT2 which catalyze similar reactions in different cellular locations. This pathway intersects with the oxidative phosphorylation process a critical energy-producing pathway within mitochondria.

NMNAT3 has implications in conditions such as anemia and mitochondrial dysfunction. Altered expression or mutation of NMNAT3 may contribute to anemia by affecting red blood cell metabolism and NAD+ homeostasis. The protein also relates to neurodegenerative disorders due to its role in mitochondrial function and energy balance aligning with proteins like Sirtuins which also regulate NAD+ levels and have implications in cellular aging processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed : 16118205, PubMed : 17402747, PubMed : 26616331). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury (PubMed : 16118205, PubMed : 17402747). May be involved in the maintenance of axonal integrity (By similarity). Also functions as a stress-response chaperone protein that prevents toxic aggregation of proteins; this function may be independent of its NAD(+) synthesis activity (PubMed : 18344983).
See full target information NMNAT3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 11: PubMed36010498

2022

Si-Wu Water Extracts Protect against Colonic Mucus Barrier Damage by Regulating Muc2 Mucin Expression in Mice Fed a High-Fat Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Ruan,Yujuan Yu,Peiheng Han,Li Zhang,Zhongyi Hu
View all publications
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Product promise

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