JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB71904

Anti-NMNAT3 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Mouse Polyclonal NMNAT3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NMNAT3.

View Alternative Names

FKSG76, NMNAT3, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3, NMN/NaMN adenylyltransferase 3, Nicotinamide-nucleotide adenylyltransferase 3, Nicotinate-nucleotide adenylyltransferase 3, Pyridine nucleotide adenylyltransferase 3, NMN adenylyltransferase 3, NaMN adenylyltransferase 3, PNAT-3

1 Images
Western blot - Anti-NMNAT3 antibody (AB71904)
  • WB

Unknown

Western blot - Anti-NMNAT3 antibody (AB71904)

All lanes:

Western blot - Anti-NMNAT3 antibody (ab71904) at 1/500 dilution

Lane 1:

NMNAT3 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 28 kDa

Observed band size: 27 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NMNAT3.

Q96T66

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMNAT3 or nicotinamide nucleotide adenylyltransferase 3 catalyzes the biosynthesis of NAD+ from NMN and ATP playing a role in cellular energy metabolism. Alternate names include NMNAT3p and Enzyme NIC. It has a molecular mass of around 31 kDa. NMNAT3 is expressed in a variety of tissues with significant expression in red blood cells and the mitochondria of other cell types.
Biological function summary

NMNAT3 participates in the NAD+ salvage pathway transforming NMN into NAD+ a fundamental coenzyme in cellular metabolism and redox reactions. It does not form part of any larger complex but functions independently within the cell. Its activity supports energy production DNA repair and cell signaling processes making it an important player in maintaining cellular homeostasis.

Pathways

The salvage pathway for NAD+ biosynthesis relies heavily on NMNAT3's enzymatic action. It works alongside related proteins like NMNAT1 and NMNAT2 which catalyze similar reactions in different cellular locations. This pathway intersects with the oxidative phosphorylation process a critical energy-producing pathway within mitochondria.

NMNAT3 has implications in conditions such as anemia and mitochondrial dysfunction. Altered expression or mutation of NMNAT3 may contribute to anemia by affecting red blood cell metabolism and NAD+ homeostasis. The protein also relates to neurodegenerative disorders due to its role in mitochondrial function and energy balance aligning with proteins like Sirtuins which also regulate NAD+ levels and have implications in cellular aging processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD(+), NADH, NaAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following injury.
See full target information NMNAT3
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadr8648 PubMed40106551

2025

PPDPF preserves integrity of proximal tubule by modulating NMNAT activity in chronic kidney diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Fang,Yi Zhong,Rui Zheng,Qihui Wu,Yu Liu,Dexin Zhang,Yuwei Wang,Wubing Ding,Kaiyuan Wang,Fengbo Zhong,Kai Lin,Xiaohui Yao,Qingxun Hu,Xiaofei Li,Guofeng Xu,Na Liu,Jing Nie,Dali Li,Hongquan Geng,Yuting Guan

Human molecular genetics 31:2918-2933 PubMed35397003

2022

Alpha-synucleinopathy reduces NMNAT3 protein levels and neurite formation that can be rescued by targeting the NAD+ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Richard B Parsons,Altin Kocinaj,Gustavo Ruiz Pulido,Sarah A Prendergast,Anna E Parsons,Paul D Facey,Frank Hirth

Frontiers in aging neuroscience 14:852972 PubMed35401143

2022

Human Nmnat1 Promotes Autophagic Clearance of Amyloid Plaques in a Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhu,Amanda G Lobato,R Grace Zhai,Milena Pinto
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB121030

Anti-NMNAT3 antibody

Western blot - Anti-NMNAT3 antibody (AB121030)

  • 0
  • 0
Primary Antibodies

AB230839

Anti-NMNAT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMNAT3 antibody (AB230839)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB56980

Anti-NMNAT2 antibody [2E4]

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-NMNAT2 antibody [2E4] (AB56980)

  • 4
  • 1
Primary Antibodies

AB24170

Anti-LAMP1 antibody - Lysosome Marker

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody - Lysosome Marker (AB24170)

  • 4
  • 100
Primary Antibodies

AB211529

Anti-NAPRT1 antibody [CL0665]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPRT1 antibody [CL0665] (AB211529)

  • 4
  • 1
Primary Antibodies

AB118270

Anti-Nmnat1/NMNAT antibody [OTI1F7]

Immunocytochemistry/ Immunofluorescence - Anti-Nmnat1/NMNAT antibody [OTI1F7] (AB118270)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com