Rabbit Polyclonal NMRAL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NMRAL1 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NMRAL1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HBL8
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
Alternative names
HSCARG, NMRAL1, NmrA-like family domain-containing protein 1
Recommended products
Rabbit Polyclonal NMRAL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NMRAL1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NMRAL1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HBL8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NMRAL1 also known as Nuclear receptor coactivator 5 (Ncoa5) is a protein involved in cellular response to oxidative stress. The encoded protein weighing approximately 42 kDa localizes to the cytoplasm and nucleus and shows widespread expression in various tissues. Researchers often study NMRAL1 because of its potential interactions with oxidized nicotinamide adenine dinucleotide (NAD+) levels affecting cellular responses.
- Biological function summary
The NMRAL1 protein assists in regulating oxidative stress responses and energy metabolism in cells. This protein may form part of multiprotein complexes that aid in transcriptional regulation by interacting with other transcription factors. It modulates cellular antioxidant responses indirectly influencing gene expression related to stress and metabolism. Scientists observe its high expression in cells facing oxidative challenges suggesting a role in protecting cellular integrity.
- Pathways
NMRAL1 has key functions in energy metabolism and redox signaling. It fits into the oxidative stress response pathway working alongside Nrf2 a critical transcription factor that regulates antioxidant proteins. The involvement of NMRAL1 in modulating redox balance highlights its interconnection with proteins like SIRT1 which also regulate cellular metabolism and oxidative stress.
- Associated diseases and disorders
NMRAL1 shows connections to metabolic diseases such as diabetes where oxidative stress plays a significant role. The protein also links to cancer where it affects tumor development through modulation of oxidative stress pathways. NMRAL1 interacts indirectly with proteins like p53 in cancer influencing cell cycle regulation and survival in response to oxidative damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NMRAL1 antibody (ab272637)
All lanes: Western blot - Anti-NMRAL1 antibody (ab272637) at 0.4 µg/mL
Lane 1: Control (vector only transfected HEK-293T lysate)
Lane 2: NMRAL1 over-expression lysate (mammalian HEK-293T cells)
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMRAL1 antibody (ab272637)
Paraffin-embedded formalin-fixed Human rectum tissue stained for NMRAL1 using ab272637 at 1/200 in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-NMRAL1 antibody (ab272637)
PFA fixed, Triton X-100 permeablized human cell line MCF7 labeling NMRAL1 (green) using ab272637 at 2 ug/ml in ICC/IF analysis.
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