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AB272637

Anti-NMRAL1 antibody

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Rabbit Polyclonal NMRAL1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NMRAL1 aa 1-150.

View Alternative Names

HSCARG, NMRAL1, NmrA-like family domain-containing protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMRAL1 antibody (AB272637)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMRAL1 antibody (AB272637)

Paraffin-embedded formalin-fixed Human rectum tissue stained for NMRAL1 using ab272637 at 1/200 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NMRAL1 antibody (AB272637)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NMRAL1 antibody (AB272637)

PFA fixed, Triton X-100 permeablized human cell line MCF7 labeling NMRAL1 (green) using ab272637 at 2 ug/ml in ICC/IF analysis.

Western blot - Anti-NMRAL1 antibody (AB272637)
  • WB

Unknown

Western blot - Anti-NMRAL1 antibody (AB272637)

All lanes:

Western blot - Anti-NMRAL1 antibody (ab272637) at 0.4 µg/mL

Lane 1:

Control (vector only transfected HEK-293T lysate)

Lane 2:

NMRAL1 over-expression lysate (mammalian HEK-293T cells)

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NMRAL1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9HBL8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NMRAL1 also known as Nuclear receptor coactivator 5 (Ncoa5) is a protein involved in cellular response to oxidative stress. The encoded protein weighing approximately 42 kDa localizes to the cytoplasm and nucleus and shows widespread expression in various tissues. Researchers often study NMRAL1 because of its potential interactions with oxidized nicotinamide adenine dinucleotide (NAD+) levels affecting cellular responses.
Biological function summary

The NMRAL1 protein assists in regulating oxidative stress responses and energy metabolism in cells. This protein may form part of multiprotein complexes that aid in transcriptional regulation by interacting with other transcription factors. It modulates cellular antioxidant responses indirectly influencing gene expression related to stress and metabolism. Scientists observe its high expression in cells facing oxidative challenges suggesting a role in protecting cellular integrity.

Pathways

NMRAL1 has key functions in energy metabolism and redox signaling. It fits into the oxidative stress response pathway working alongside Nrf2 a critical transcription factor that regulates antioxidant proteins. The involvement of NMRAL1 in modulating redox balance highlights its interconnection with proteins like SIRT1 which also regulate cellular metabolism and oxidative stress.

NMRAL1 shows connections to metabolic diseases such as diabetes where oxidative stress plays a significant role. The protein also links to cancer where it affects tumor development through modulation of oxidative stress pathways. NMRAL1 interacts indirectly with proteins like p53 in cancer influencing cell cycle regulation and survival in response to oxidative damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
See full target information NMRAL1
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Product promise

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For full details, please see our Terms & Conditions

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