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AB102473

Anti-NMT2 antibody

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(1 Publication)

Rabbit Polyclonal NMT2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glycylpeptide N-tetradecanoyltransferase 2 aa 1-250.

View Alternative Names

Glycylpeptide N-tetradecanoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Peptide N-myristoyltransferase 2, Protein-lysine myristoyltransferase NMT2, Type II N-myristoyltransferase, NMT 2, NMT2

1 Images
Western blot - Anti-NMT2 antibody (AB102473)
  • WB

Unknown

Western blot - Anti-NMT2 antibody (AB102473)

All lanes:

Western blot - Anti-NMT2 antibody (ab102473) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 57 kDa,63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Glycylpeptide N-tetradecanoyltransferase 2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O60551

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-myristoyltransferase 2 or NMT2 is an enzyme that catalyzes N-terminal myristoylation. It transfers myristic acid a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This modification influences the protein's function stability and subcellular localization. NMT2 has a structure with an approximate mass of 53 kDa. It is expressed in various tissues including the liver and the brain contributing to diverse biological processes.
Biological function summary

NMT2 participates in the post-translational modification of proteins which impacts cellular signaling and protein-protein interactions. It exists within a myristoylation enzyme complex working alongside its isoform NMT1 which complements its activity. NMT2 influences pathways involving protein trafficking and cell growth playing a role in membrane localization and signal transduction of several key proteins.

Pathways

NMT2 actively engages in the Wnt signaling and protein kinase C (PKC) pathways. These pathways contribute to regulating cell fate decisions and cellular responses to extracellular signals. NMT2 shares pathway interaction with proteins like beta-catenin in the Wnt pathway and is linked with PKC isoforms within the PKC pathway. This involvement affects cytoskeleton organization cellular proliferation and apoptosis.

Scientists have observed that alterations in NMT2 activity associate with various diseases including cancer and neurological disorders. In cancer abnormal NMT2 expression impacts cancer cell survival and metastasis. In neurological disorders changes in myristoylation affect synaptic function and neuronal signaling. NMT2 interacts with proteins like beta-catenin in cancer progression and synaptophysin in neurological conditions. These connections highlight NMT2 as a potential therapeutic target for drug development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 25255805, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).
See full target information Glycylpeptide N-tetradecanoyltransferase 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 2:462-9 PubMed22939983

2012

Repression of transcription by WT1-BASP1 requires the myristoylation of BASP1 and the PIP2-dependent recruitment of histone deacetylase.

Applications

IF

Species

Unspecified reactive species

Eneda Toska,Hayley A Campbell,Jayasha Shandilya,Sarah J Goodfellow,Paul Shore,Kathryn F Medler,Stefan G E Roberts
View all publications
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Product promise

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