AB230028

Anti-NMT2 antibody

Name: Anti-NMT2 antibody (ab230028) | Abcam
Brand: Abcam Limited
SKU: AB230028
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NMT2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glycylpeptide N-tetradecanoyltransferase 2 aa 1-150.

View Alternative Names

Glycylpeptide N-tetradecanoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Peptide N-myristoyltransferase 2, Protein-lysine myristoyltransferase NMT2, Type II N-myristoyltransferase, NMT 2, NMT2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (AB230028)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (AB230028)

Paraffin-embedded human testis tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (AB230028)

Paraffin-embedded human prostate tissue stained for NMT2 with ab230028 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-NMT2 antibody (AB230028)

NMT2 was immunoprecipitated from 0.5 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate using ab230028.

Lane 1 : Control IgG IP in HepG2 whole cell lysate.

Lane 2 : ab230028 in HepG2 whole cell lysate.

Lane 3 : NMT2 in HepG2 whole cell lysate 20 μg (Input).

For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/50,000).

All lanes:

Immunoprecipitation - Anti-NMT2 antibody (ab230028)

Predicted band size: 57 kDa

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Supplier Data

Western blot - Anti-NMT2 antibody (AB230028)

All lanes:

Western blot - Anti-NMT2 antibody (ab230028) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 57 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Glycylpeptide N-tetradecanoyltransferase 2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O60551

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-myristoyltransferase 2 or NMT2 is an enzyme that catalyzes N-terminal myristoylation. It transfers myristic acid a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This modification influences the protein's function stability and subcellular localization. NMT2 has a structure with an approximate mass of 53 kDa. It is expressed in various tissues including the liver and the brain contributing to diverse biological processes.

Biological function summary

NMT2 participates in the post-translational modification of proteins which impacts cellular signaling and protein-protein interactions. It exists within a myristoylation enzyme complex working alongside its isoform NMT1 which complements its activity. NMT2 influences pathways involving protein trafficking and cell growth playing a role in membrane localization and signal transduction of several key proteins.

Pathways

NMT2 actively engages in the Wnt signaling and protein kinase C (PKC) pathways. These pathways contribute to regulating cell fate decisions and cellular responses to extracellular signals. NMT2 shares pathway interaction with proteins like beta-catenin in the Wnt pathway and is linked with PKC isoforms within the PKC pathway. This involvement affects cytoskeleton organization cellular proliferation and apoptosis.

Scientists have observed that alterations in NMT2 activity associate with various diseases including cancer and neurological disorders. In cancer abnormal NMT2 expression impacts cancer cell survival and metastasis. In neurological disorders changes in myristoylation affect synaptic function and neuronal signaling. NMT2 interacts with proteins like beta-catenin in cancer progression and synaptophysin in neurological conditions. These connections highlight NMT2 as a potential therapeutic target for drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed : 25255805, PubMed : 9506952). Also able to mediate N-terminal lysine myristoylation of proteins : catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed : 32103017). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed : 32103017).

See full target information Glycylpeptide N-tetradecanoyltransferase 2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research 77:103378 PubMed38479332

2024

Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing.

Applications

Unspecified application

Species

Unspecified reactive species

M Korneck,A Leonhardt,L Schöls,S Hauser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NMT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (AB230028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMT2 antibody (AB230028)

Immunoprecipitation - Anti-NMT2 antibody (AB230028)

Western blot - Anti-NMT2 antibody (AB230028)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise