Rabbit Recombinant Monoclonal NMT2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-NMT2 antibody [EPR24372-10] (AB270957), expandable thumbnail](https://content.abcam.com/products/images/nmt2-antibody-epr24372-10-ab270957--western-blot-img170436.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NMT2 antibody [EPR24372-10] (AB270957)")
![Western blot - Anti-NMT2 antibody [EPR24372-10] (AB270957), expandable thumbnail](https://content.abcam.com/products/images/nmt2-antibody-epr24372-10-ab270957--western-blot-img166074.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NMT2 antibody [EPR24372-10] (AB270957)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins (PubMed:25255805, PubMed:9506952). Also able to mediate N-terminal lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on both 'Gly-2' and 'Lys-3' (PubMed:32103017). Lysine myristoylation is required to maintain ARF6 on membranes during the GTPase cycle (PubMed:32103017).
Alternative names
Glycylpeptide N-tetradecanoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, Peptide N-myristoyltransferase 2, Protein-lysine myristoyltransferase NMT2, Type II N-myristoyltransferase, NMT 2, NMT2
Recommended products
Rabbit Recombinant Monoclonal NMT2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24372-10
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-myristoyltransferase 2 or NMT2 is an enzyme that catalyzes N-terminal myristoylation. It transfers myristic acid a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This modification influences the protein's function stability and subcellular localization. NMT2 has a structure with an approximate mass of 53 kDa. It is expressed in various tissues including the liver and the brain contributing to diverse biological processes.
- Biological function summary
NMT2 participates in the post-translational modification of proteins which impacts cellular signaling and protein-protein interactions. It exists within a myristoylation enzyme complex working alongside its isoform NMT1 which complements its activity. NMT2 influences pathways involving protein trafficking and cell growth playing a role in membrane localization and signal transduction of several key proteins.
- Pathways
NMT2 actively engages in the Wnt signaling and protein kinase C (PKC) pathways. These pathways contribute to regulating cell fate decisions and cellular responses to extracellular signals. NMT2 shares pathway interaction with proteins like beta-catenin in the Wnt pathway and is linked with PKC isoforms within the PKC pathway. This involvement affects cytoskeleton organization cellular proliferation and apoptosis.
- Associated diseases and disorders
Scientists have observed that alterations in NMT2 activity associate with various diseases including cancer and neurological disorders. In cancer abnormal NMT2 expression impacts cancer cell survival and metastasis. In neurological disorders changes in myristoylation affect synaptic function and neuronal signaling. NMT2 interacts with proteins like beta-catenin in cancer progression and synaptophysin in neurological conditions. These connections highlight NMT2 as a potential therapeutic target for drug development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957), expandable thumbnail](https://content.abcam.com/products/images/nmt2-antibody-epr24372-10-ab270957--western-blot-img170436.jpg "Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957)")
Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 7559471).
Low expression: HCT-15 (PMID: 16530191).
This antibody reacts with an unidentifiable protein around 36 kDa.
Exposure time: Lane 1-2: 37 seconds ; Lane 3-6: 136 seconds.
All lanes: Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HCT-15 (human colon epithelial) whole cell lysate at 20 µg
Lane 3: SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 20 µg
Lane 4: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 114 kDa, 57 kDa
![Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957), expandable thumbnail](https://content.abcam.com/products/images/nmt2-antibody-epr24372-10-ab270957--western-blot-img166074.jpg "Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957)")
Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 7559471).
This antibody reacts with an unidentifiable protein around 36 kDa.
Exposure time: Lane 1: 10 seconds ; Lane 2-4: 26 seconds.
All lanes: Western blot - Anti-NMT2 antibody [EPR24372-10] (ab270957) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 114 kDa, 57 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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